Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2022.104922 http://hdl.handle.net/11449/241746 |
Resumo: | This is a feasibility study aimed to determine the effectiveness of a culture media designed to enhance embryonic cryopreservation based on laboratory-accessed quality phenotypes of in vitro produced bovine embryos. Recovered bovine oocytes were matured and fertilized in vitro and the presumptive zygotes that were obtained were randomly distributed into three culture media: (i) Control (C): modified synthetic oviduct fluid (mSOF) culture medium supplemented with 5 mg/mL BSA and 2.5% fetal calf serum; (ii) Medium A (MA): serum-free altered mSOF medium A; and iii) Medium B (MB): serum-free altered mSOF medium B. Embryos cultured in MA and MB presented a better viability after cryopreservation (P < 0.05) without affecting the production rates of blastocysts (P > 0.05, MB only) when compared to C. The MB-derived blastocysts had the highest (P < 0.05) hatching rate (26.1%, 53/203) and higher (P < 0.05) transferable embryos rate (92.1%, 187/203) compared to control-derived blastocysts (85.8%, 175/204). The total cell number in the embryos after vitrification/warming process was greater (P < 0.05) in the MA (144.9 ± 5.9) and MB (151.3 ± 7.1) compared to that in the control (121.4 ± 6.1). The number of large and giant cytoplasmic lipid droplets was reduced (P < 0.05) in MB-derived embryos compared to control-derived embryos. Additionally, the lipid profiles of MB-derived blastocysts were closely related to those observed in the in vivo-derived blastocysts. We observed different transcriptional profiles abundance of in vitro produced bovine embryos derived from control, MA and MB groups. Therefore, the novel MB culture medium increased embryonic cryosurvival and favored the production of bovine embryos in vitro with improved cellular and molecular laboratory-accessed quality phenotypes. |
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Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryosBovine embryoCryosurvivalLipidsVitrificationThis is a feasibility study aimed to determine the effectiveness of a culture media designed to enhance embryonic cryopreservation based on laboratory-accessed quality phenotypes of in vitro produced bovine embryos. Recovered bovine oocytes were matured and fertilized in vitro and the presumptive zygotes that were obtained were randomly distributed into three culture media: (i) Control (C): modified synthetic oviduct fluid (mSOF) culture medium supplemented with 5 mg/mL BSA and 2.5% fetal calf serum; (ii) Medium A (MA): serum-free altered mSOF medium A; and iii) Medium B (MB): serum-free altered mSOF medium B. Embryos cultured in MA and MB presented a better viability after cryopreservation (P < 0.05) without affecting the production rates of blastocysts (P > 0.05, MB only) when compared to C. The MB-derived blastocysts had the highest (P < 0.05) hatching rate (26.1%, 53/203) and higher (P < 0.05) transferable embryos rate (92.1%, 187/203) compared to control-derived blastocysts (85.8%, 175/204). The total cell number in the embryos after vitrification/warming process was greater (P < 0.05) in the MA (144.9 ± 5.9) and MB (151.3 ± 7.1) compared to that in the control (121.4 ± 6.1). The number of large and giant cytoplasmic lipid droplets was reduced (P < 0.05) in MB-derived embryos compared to control-derived embryos. Additionally, the lipid profiles of MB-derived blastocysts were closely related to those observed in the in vivo-derived blastocysts. We observed different transcriptional profiles abundance of in vitro produced bovine embryos derived from control, MA and MB groups. Therefore, the novel MB culture medium increased embryonic cryosurvival and favored the production of bovine embryos in vitro with improved cellular and molecular laboratory-accessed quality phenotypes.School of Veterinary Medicine Federal University of Pampa, RSIn Vitro Brasil ABS Pecplan, SPCenter for Natural and Human Sciences Federal University of ABC, SPInvitra – Assisted Reproductive Technologies Ltd. Supera Innovation and Technology Park, São PauloInstitute of Chemistry Federal University of Catalão UFG / UFCAT, GOThoMSon Mass Spectrometry Laboratory Institute of Chemistry University of Campinas, SPSchool of Engineering Mackenzie Presbyterian University, SPMulti-user Laboratory of Phytomedicines Pharmacology and Biotechnology (PhitoPharmaTec) Department of Pharmacology São Paulo State University Institute of BiosciencesDepartment of Biological Sciences School of Sciences and Languages São Paulo State UniversityDepartment of Genetics and Evolution Federal University of São Carlos, São PauloMulti-user Laboratory of Phytomedicines Pharmacology and Biotechnology (PhitoPharmaTec) Department of Pharmacology São Paulo State University Institute of BiosciencesDepartment of Biological Sciences School of Sciences and Languages São Paulo State UniversityFederal University of PampaABS PecplanFederal University of ABCSupera Innovation and Technology ParkUFG / UFCATUniversidade Estadual de Campinas (UNICAMP)Mackenzie Presbyterian UniversityUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)de Camargo, JanineRodrigues, RafaelaValente, Roniele SantanaMuller, Diego BorbaVireque, Alessandra AparecidaBelaz, Katia Roberta AnacletoBohrer, Rodrigo CamponogaraBasso, Andrea CristinaEberlin, Marcos NogueiraFontes, Patricia Kubo [UNESP]Nogueira, Marcelo Fábio Gouveia [UNESP]Sudano, Mateus José2023-03-01T23:40:28Z2023-03-01T23:40:28Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.livsci.2022.104922Livestock Science, v. 260.1871-1413http://hdl.handle.net/11449/24174610.1016/j.livsci.2022.1049222-s2.0-85128448088Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Scienceinfo:eu-repo/semantics/openAccess2023-03-01T23:40:28Zoai:repositorio.unesp.br:11449/241746Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T23:40:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
title |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
spellingShingle |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos de Camargo, Janine Bovine embryo Cryosurvival Lipids Vitrification |
title_short |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
title_full |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
title_fullStr |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
title_full_unstemmed |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
title_sort |
Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos |
author |
de Camargo, Janine |
author_facet |
de Camargo, Janine Rodrigues, Rafaela Valente, Roniele Santana Muller, Diego Borba Vireque, Alessandra Aparecida Belaz, Katia Roberta Anacleto Bohrer, Rodrigo Camponogara Basso, Andrea Cristina Eberlin, Marcos Nogueira Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Sudano, Mateus José |
author_role |
author |
author2 |
Rodrigues, Rafaela Valente, Roniele Santana Muller, Diego Borba Vireque, Alessandra Aparecida Belaz, Katia Roberta Anacleto Bohrer, Rodrigo Camponogara Basso, Andrea Cristina Eberlin, Marcos Nogueira Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Sudano, Mateus José |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Pampa ABS Pecplan Federal University of ABC Supera Innovation and Technology Park UFG / UFCAT Universidade Estadual de Campinas (UNICAMP) Mackenzie Presbyterian University Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
de Camargo, Janine Rodrigues, Rafaela Valente, Roniele Santana Muller, Diego Borba Vireque, Alessandra Aparecida Belaz, Katia Roberta Anacleto Bohrer, Rodrigo Camponogara Basso, Andrea Cristina Eberlin, Marcos Nogueira Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Sudano, Mateus José |
dc.subject.por.fl_str_mv |
Bovine embryo Cryosurvival Lipids Vitrification |
topic |
Bovine embryo Cryosurvival Lipids Vitrification |
description |
This is a feasibility study aimed to determine the effectiveness of a culture media designed to enhance embryonic cryopreservation based on laboratory-accessed quality phenotypes of in vitro produced bovine embryos. Recovered bovine oocytes were matured and fertilized in vitro and the presumptive zygotes that were obtained were randomly distributed into three culture media: (i) Control (C): modified synthetic oviduct fluid (mSOF) culture medium supplemented with 5 mg/mL BSA and 2.5% fetal calf serum; (ii) Medium A (MA): serum-free altered mSOF medium A; and iii) Medium B (MB): serum-free altered mSOF medium B. Embryos cultured in MA and MB presented a better viability after cryopreservation (P < 0.05) without affecting the production rates of blastocysts (P > 0.05, MB only) when compared to C. The MB-derived blastocysts had the highest (P < 0.05) hatching rate (26.1%, 53/203) and higher (P < 0.05) transferable embryos rate (92.1%, 187/203) compared to control-derived blastocysts (85.8%, 175/204). The total cell number in the embryos after vitrification/warming process was greater (P < 0.05) in the MA (144.9 ± 5.9) and MB (151.3 ± 7.1) compared to that in the control (121.4 ± 6.1). The number of large and giant cytoplasmic lipid droplets was reduced (P < 0.05) in MB-derived embryos compared to control-derived embryos. Additionally, the lipid profiles of MB-derived blastocysts were closely related to those observed in the in vivo-derived blastocysts. We observed different transcriptional profiles abundance of in vitro produced bovine embryos derived from control, MA and MB groups. Therefore, the novel MB culture medium increased embryonic cryosurvival and favored the production of bovine embryos in vitro with improved cellular and molecular laboratory-accessed quality phenotypes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-01 2023-03-01T23:40:28Z 2023-03-01T23:40:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2022.104922 Livestock Science, v. 260. 1871-1413 http://hdl.handle.net/11449/241746 10.1016/j.livsci.2022.104922 2-s2.0-85128448088 |
url |
http://dx.doi.org/10.1016/j.livsci.2022.104922 http://hdl.handle.net/11449/241746 |
identifier_str_mv |
Livestock Science, v. 260. 1871-1413 10.1016/j.livsci.2022.104922 2-s2.0-85128448088 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789897619144704 |