IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1089-6891v20e-34543 http://hdl.handle.net/11449/183804 |
Resumo: | Abstract The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate. |
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Repositório Institucional da UNESP |
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IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVASIMMUNOEXPRESSION OF TGF-Β IN NORMAL CANINE PROSTATES AND WITH PROLIFERATIVE LESIONScarcinomaimmunohistochemistrygrowth factorPIAPINcarcinomafator de crescimentoimunoistoquímicaPIAPINAbstract The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate.Resumo O fator de crescimento transformador-β (TGF-β), um mediador do crescimento prostático, induz a angiogênese e inibe a proliferação celular. Neste estudo, esse marcador foi utilizado com o objetivo de avaliar sua imunomarcação no tecido normal e com lesões proliferativas benignas, pré-neoplásicas e neoplásicas da próstata canina. Para isso, foram selecionadas 54 glândulas com histomorfologia normal, hiperplasia prostática benigna (HPB) epitelial, HPB estromal, atrofia inflamatória proliferativa (PIA), neoplasia intraepitelial prostática (PIN) e carcinoma, utilizadas para a confecção de um bloco de microarranjo tecidual (Tissue Microarray - TMA). As lâminas de TMA foram submetidas à técnica de imunoistoquímica com o anticorpo anti-TGF-β, sendo avaliada a intensidade de imunomarcação nas células epiteliais e estromais. Houve imunomarcação de TGF-β no tecido normal e naqueles com lesões proliferativas. Maior imunomarcação de TGF-β foi constatada nas células do tecido prostático normal e com HPB, enquanto as células prostáticas com PIA, PIN e carcinoma exibiram menor imunomarcação dessa citocina, o que sugere a ação do TGF-β na manutenção da homeostase do tecido normal e com lesão proliferativa benigna e na progressão das lesões proliferativas pré-malignas e malignas da próstata canina.Universidade Federal de GoiáUNESP Faculdade de Medicina Veterinária e ZootecniaUNESP Faculdade de Medicina Veterinária e ZootecniaUniversidade Federal de GoiásUniversidade Federal de GoiáUniversidade Estadual Paulista (Unesp)Toledo, Denise CarolineFaleiro, Mariana Batista RodriguesFerreira, Hugo HenriqueFaria, Adriana MarquesMatos, Moema Pacheco ChediakAmorim, Renee LauferMoura, Veridiana Maria Brianezi Dignani De2019-10-03T17:31:43Z2019-10-03T17:31:43Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1089-6891v20e-34543Ciência Animal Brasileira. Universidade Federal de Goiás, v. 20, p. -, 2019.1809-6891http://hdl.handle.net/11449/18380410.1590/1089-6891v20e-34543S1809-68912019000100317S1809-68912019000100317.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporCiência Animal Brasileirainfo:eu-repo/semantics/openAccess2023-12-23T06:15:43Zoai:repositorio.unesp.br:11449/183804Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-23T06:15:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS IMMUNOEXPRESSION OF TGF-Β IN NORMAL CANINE PROSTATES AND WITH PROLIFERATIVE LESIONS |
title |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS |
spellingShingle |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS Toledo, Denise Caroline carcinoma immunohistochemistry growth factor PIA PIN carcinoma fator de crescimento imunoistoquímica PIA PIN |
title_short |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS |
title_full |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS |
title_fullStr |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS |
title_full_unstemmed |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS |
title_sort |
IMUNOMARCAÇÃO DE TGF-β EM PRÓSTATAS CANINAS NORMAIS E COM LESÕES PROLIFERATIVAS |
author |
Toledo, Denise Caroline |
author_facet |
Toledo, Denise Caroline Faleiro, Mariana Batista Rodrigues Ferreira, Hugo Henrique Faria, Adriana Marques Matos, Moema Pacheco Chediak Amorim, Renee Laufer Moura, Veridiana Maria Brianezi Dignani De |
author_role |
author |
author2 |
Faleiro, Mariana Batista Rodrigues Ferreira, Hugo Henrique Faria, Adriana Marques Matos, Moema Pacheco Chediak Amorim, Renee Laufer Moura, Veridiana Maria Brianezi Dignani De |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiá Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Toledo, Denise Caroline Faleiro, Mariana Batista Rodrigues Ferreira, Hugo Henrique Faria, Adriana Marques Matos, Moema Pacheco Chediak Amorim, Renee Laufer Moura, Veridiana Maria Brianezi Dignani De |
dc.subject.por.fl_str_mv |
carcinoma immunohistochemistry growth factor PIA PIN carcinoma fator de crescimento imunoistoquímica PIA PIN |
topic |
carcinoma immunohistochemistry growth factor PIA PIN carcinoma fator de crescimento imunoistoquímica PIA PIN |
description |
Abstract The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-03T17:31:43Z 2019-10-03T17:31:43Z 2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1089-6891v20e-34543 Ciência Animal Brasileira. Universidade Federal de Goiás, v. 20, p. -, 2019. 1809-6891 http://hdl.handle.net/11449/183804 10.1590/1089-6891v20e-34543 S1809-68912019000100317 S1809-68912019000100317.pdf |
url |
http://dx.doi.org/10.1590/1089-6891v20e-34543 http://hdl.handle.net/11449/183804 |
identifier_str_mv |
Ciência Animal Brasileira. Universidade Federal de Goiás, v. 20, p. -, 2019. 1809-6891 10.1590/1089-6891v20e-34543 S1809-68912019000100317 S1809-68912019000100317.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Ciência Animal Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790082683371520 |