Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2019.104053 http://hdl.handle.net/11449/199620 |
Resumo: | The enteric protist Blastocystis is one of the most commonly parasite reported in humans and a variety of animal hosts worldwide. Regarding genetic diversity, at least 17 subtypes (STs) have been identified in mammals and birds, with eight of them (ST1-8) infecting both humans and animals. Recently, isolates from wild mammalian species have been genetically characterized, however data is still scarce, mainly in Latin America. Here, we aimed to verify the occurrence and genetic diversity of Blastocystis in captive wild mammals kept in one zoo and in two units of protection and conservation in southeastern Brazil. A total of 78 fecal samples (14 pooled and 64 individual samples) were recovered from 102 wild mammals of 35 species included in the following orders: Primates, Carnivora, Artiodactyla, Pilosa, Rodentia and Marsupialia. Zoo and units staff were invited to participated but only 16 fecal samples could be screened. Based on the sequence analyses of SSUrDNA gene, out of 29 PCR products from animal samples, 51.7% (15/29) were successfully sequenced and five Blastocystis subtypes were identified as follows: ST1 (2/15; 13.3%), ST2 (2/15; 13.3%), ST3 (4/15; 26.6%), ST5 (2/15; 13.3%) and ST8 (5/14; 33.3%). Only four isolates from humans were sequenced and identified as ST1 (2 isolates), ST2 and ST3. It was observed that Blastocystis infecting non-human primates belong to ST1 and ST2 and mainly to ST3 and ST8, artiodactyls ST5, carnivores ST1 and ST5 and rodents ST1. In addition, this present study reports some interesting findings: (1) 63% (12/19) of Blastocystis isolates from animals and employees belonged to the potentially zoonotic subtypes ST1-ST3; (2) most of these isolates displayed high identity with publicly available DNA sequences from non-human primates and humans, including primate handlers; (3) Blastocystis ST5 was found infecting the northern tiger cat, a native South American felid and one of the species facing a high risk of extinction in Brazil. |
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Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern BrazilThe enteric protist Blastocystis is one of the most commonly parasite reported in humans and a variety of animal hosts worldwide. Regarding genetic diversity, at least 17 subtypes (STs) have been identified in mammals and birds, with eight of them (ST1-8) infecting both humans and animals. Recently, isolates from wild mammalian species have been genetically characterized, however data is still scarce, mainly in Latin America. Here, we aimed to verify the occurrence and genetic diversity of Blastocystis in captive wild mammals kept in one zoo and in two units of protection and conservation in southeastern Brazil. A total of 78 fecal samples (14 pooled and 64 individual samples) were recovered from 102 wild mammals of 35 species included in the following orders: Primates, Carnivora, Artiodactyla, Pilosa, Rodentia and Marsupialia. Zoo and units staff were invited to participated but only 16 fecal samples could be screened. Based on the sequence analyses of SSUrDNA gene, out of 29 PCR products from animal samples, 51.7% (15/29) were successfully sequenced and five Blastocystis subtypes were identified as follows: ST1 (2/15; 13.3%), ST2 (2/15; 13.3%), ST3 (4/15; 26.6%), ST5 (2/15; 13.3%) and ST8 (5/14; 33.3%). Only four isolates from humans were sequenced and identified as ST1 (2 isolates), ST2 and ST3. It was observed that Blastocystis infecting non-human primates belong to ST1 and ST2 and mainly to ST3 and ST8, artiodactyls ST5, carnivores ST1 and ST5 and rodents ST1. In addition, this present study reports some interesting findings: (1) 63% (12/19) of Blastocystis isolates from animals and employees belonged to the potentially zoonotic subtypes ST1-ST3; (2) most of these isolates displayed high identity with publicly available DNA sequences from non-human primates and humans, including primate handlers; (3) Blastocystis ST5 was found infecting the northern tiger cat, a native South American felid and one of the species facing a high risk of extinction in Brazil.Department of Parasitology Institute of Bioscience São Paulo State University (UNESP)Integrated Faculties of Bauru (FIB)Sagrado Coração University (USC) Department of Health SciencesTropical Diseases Postgraduate Program Medical School São Paulo State University (UNESP)Department of Zoology Institute of Bioscience São Paulo State University (UNESP)Paulista Agency of Agribusiness Technology (APTA/SAA)Department of Parasitology Institute of Bioscience São Paulo State University (UNESP)Tropical Diseases Postgraduate Program Medical School São Paulo State University (UNESP)Department of Zoology Institute of Bioscience São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Integrated Faculties of Bauru (FIB)Sagrado Coração University (USC)Paulista Agency of Agribusiness Technology (APTA/SAA)Oliveira-Arbex, Ana Paula [UNESP]David, Érica Boarato [UNESP]Tenório, Michely da Silva [UNESP]Cicchi, Paulo José Pyles [UNESP]Patti, MariellaCoradi, Silvana TorossianLucheis, Simone BaldiniJim, Jorge [UNESP]Guimarães, Semíramis [UNESP]2020-12-12T01:44:51Z2020-12-12T01:44:51Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.meegid.2019.104053Infection, Genetics and Evolution, v. 78.1567-72571567-1348http://hdl.handle.net/11449/19962010.1016/j.meegid.2019.1040532-s2.0-85074626837Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection, Genetics and Evolutioninfo:eu-repo/semantics/openAccess2021-10-23T08:46:34Zoai:repositorio.unesp.br:11449/199620Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:46:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
title |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
spellingShingle |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil Oliveira-Arbex, Ana Paula [UNESP] |
title_short |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
title_full |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
title_fullStr |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
title_full_unstemmed |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
title_sort |
Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil |
author |
Oliveira-Arbex, Ana Paula [UNESP] |
author_facet |
Oliveira-Arbex, Ana Paula [UNESP] David, Érica Boarato [UNESP] Tenório, Michely da Silva [UNESP] Cicchi, Paulo José Pyles [UNESP] Patti, Mariella Coradi, Silvana Torossian Lucheis, Simone Baldini Jim, Jorge [UNESP] Guimarães, Semíramis [UNESP] |
author_role |
author |
author2 |
David, Érica Boarato [UNESP] Tenório, Michely da Silva [UNESP] Cicchi, Paulo José Pyles [UNESP] Patti, Mariella Coradi, Silvana Torossian Lucheis, Simone Baldini Jim, Jorge [UNESP] Guimarães, Semíramis [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Integrated Faculties of Bauru (FIB) Sagrado Coração University (USC) Paulista Agency of Agribusiness Technology (APTA/SAA) |
dc.contributor.author.fl_str_mv |
Oliveira-Arbex, Ana Paula [UNESP] David, Érica Boarato [UNESP] Tenório, Michely da Silva [UNESP] Cicchi, Paulo José Pyles [UNESP] Patti, Mariella Coradi, Silvana Torossian Lucheis, Simone Baldini Jim, Jorge [UNESP] Guimarães, Semíramis [UNESP] |
description |
The enteric protist Blastocystis is one of the most commonly parasite reported in humans and a variety of animal hosts worldwide. Regarding genetic diversity, at least 17 subtypes (STs) have been identified in mammals and birds, with eight of them (ST1-8) infecting both humans and animals. Recently, isolates from wild mammalian species have been genetically characterized, however data is still scarce, mainly in Latin America. Here, we aimed to verify the occurrence and genetic diversity of Blastocystis in captive wild mammals kept in one zoo and in two units of protection and conservation in southeastern Brazil. A total of 78 fecal samples (14 pooled and 64 individual samples) were recovered from 102 wild mammals of 35 species included in the following orders: Primates, Carnivora, Artiodactyla, Pilosa, Rodentia and Marsupialia. Zoo and units staff were invited to participated but only 16 fecal samples could be screened. Based on the sequence analyses of SSUrDNA gene, out of 29 PCR products from animal samples, 51.7% (15/29) were successfully sequenced and five Blastocystis subtypes were identified as follows: ST1 (2/15; 13.3%), ST2 (2/15; 13.3%), ST3 (4/15; 26.6%), ST5 (2/15; 13.3%) and ST8 (5/14; 33.3%). Only four isolates from humans were sequenced and identified as ST1 (2 isolates), ST2 and ST3. It was observed that Blastocystis infecting non-human primates belong to ST1 and ST2 and mainly to ST3 and ST8, artiodactyls ST5, carnivores ST1 and ST5 and rodents ST1. In addition, this present study reports some interesting findings: (1) 63% (12/19) of Blastocystis isolates from animals and employees belonged to the potentially zoonotic subtypes ST1-ST3; (2) most of these isolates displayed high identity with publicly available DNA sequences from non-human primates and humans, including primate handlers; (3) Blastocystis ST5 was found infecting the northern tiger cat, a native South American felid and one of the species facing a high risk of extinction in Brazil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:44:51Z 2020-12-12T01:44:51Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2019.104053 Infection, Genetics and Evolution, v. 78. 1567-7257 1567-1348 http://hdl.handle.net/11449/199620 10.1016/j.meegid.2019.104053 2-s2.0-85074626837 |
url |
http://dx.doi.org/10.1016/j.meegid.2019.104053 http://hdl.handle.net/11449/199620 |
identifier_str_mv |
Infection, Genetics and Evolution, v. 78. 1567-7257 1567-1348 10.1016/j.meegid.2019.104053 2-s2.0-85074626837 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection, Genetics and Evolution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
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Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965663018614784 |