Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2021.106764 http://hdl.handle.net/11449/210374 |
Resumo: | This study was conducted to evaluate the luteinizing hormone (LH) secretion pattern after gamma-aminobutyric acid (GABA(A)) antagonist to determine the effects of the GABAergic system on LH secretion during reproductive maturation in pre-pubertal Nellore heifers. Nellore heifers (n = 10) were administered a picrotoxin injection of 0.18 mg/kg, i.v. Blood samples were collected every 15 min for 3 h at different developmental stages (8, 10, 14 and 17 mo of age). Plasma concentrations of LH were quantified using an RIA (sensitivity of 0.04 ng/mL and CV of 15 %). There was an interaction between treatment and age (P = 0.034). Picrotoxin-treated heifers had lesser (P <= 0.05) LH mean concentrations during a 3 h period at 10 and 17 mo of age compared to control heifers (P <= 0.05). Comparing the period before and after Picrotoxin injection in the same animals, there was a 33 % decrease in LH concentration at 8 mo of age (P = 0.0165). These results indicate the GABAergic system has a stimulatory function in inducing LH secretion in pre-pubertal Nellore heifers. These findings corroborate previous results that GABA increases GnRH/LH secretion in other species during the pre-pubertal period. |
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Repositório Institucional da UNESP |
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Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifersHypothalamusNeuroendocrinePicrotoxinPubertySexual maturationThis study was conducted to evaluate the luteinizing hormone (LH) secretion pattern after gamma-aminobutyric acid (GABA(A)) antagonist to determine the effects of the GABAergic system on LH secretion during reproductive maturation in pre-pubertal Nellore heifers. Nellore heifers (n = 10) were administered a picrotoxin injection of 0.18 mg/kg, i.v. Blood samples were collected every 15 min for 3 h at different developmental stages (8, 10, 14 and 17 mo of age). Plasma concentrations of LH were quantified using an RIA (sensitivity of 0.04 ng/mL and CV of 15 %). There was an interaction between treatment and age (P = 0.034). Picrotoxin-treated heifers had lesser (P <= 0.05) LH mean concentrations during a 3 h period at 10 and 17 mo of age compared to control heifers (P <= 0.05). Comparing the period before and after Picrotoxin injection in the same animals, there was a 33 % decrease in LH concentration at 8 mo of age (P = 0.0165). These results indicate the GABAergic system has a stimulatory function in inducing LH secretion in pre-pubertal Nellore heifers. These findings corroborate previous results that GABA increases GnRH/LH secretion in other species during the pre-pubertal period.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo Agcy Agribusiness Technol, Secretary Agr & Food Supply, Biol Inst, Sao Paulo, BrazilTexas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USASao Paulo State Univ, Dept Anim Prod & Hlth, Aracatuba, BrazilSao Paulo State Univ, Dept Anim Prod & Hlth, Aracatuba, BrazilFAPESP: 99/10446-7Elsevier B.V.Sao Paulo Agcy Agribusiness TechnolTexas A&M UnivUniversidade Estadual Paulista (Unesp)Cardoso, DanielCardoso, Rodolfo C.Nogueira, Guilherme de Paula [UNESP]2021-06-25T15:06:26Z2021-06-25T15:06:26Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6http://dx.doi.org/10.1016/j.anireprosci.2021.106764Animal Reproduction Science. Amsterdam: Elsevier, v. 229, 6 p., 2021.0378-4320http://hdl.handle.net/11449/21037410.1016/j.anireprosci.2021.106764WOS:000655469600007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-23T20:17:27Zoai:repositorio.unesp.br:11449/210374Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:17:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
title |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
spellingShingle |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers Cardoso, Daniel Hypothalamus Neuroendocrine Picrotoxin Puberty Sexual maturation |
title_short |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
title_full |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
title_fullStr |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
title_full_unstemmed |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
title_sort |
Functions of the GABAergic system on serum LH concentrations in pre-pubertal Nellore heifers |
author |
Cardoso, Daniel |
author_facet |
Cardoso, Daniel Cardoso, Rodolfo C. Nogueira, Guilherme de Paula [UNESP] |
author_role |
author |
author2 |
Cardoso, Rodolfo C. Nogueira, Guilherme de Paula [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Sao Paulo Agcy Agribusiness Technol Texas A&M Univ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cardoso, Daniel Cardoso, Rodolfo C. Nogueira, Guilherme de Paula [UNESP] |
dc.subject.por.fl_str_mv |
Hypothalamus Neuroendocrine Picrotoxin Puberty Sexual maturation |
topic |
Hypothalamus Neuroendocrine Picrotoxin Puberty Sexual maturation |
description |
This study was conducted to evaluate the luteinizing hormone (LH) secretion pattern after gamma-aminobutyric acid (GABA(A)) antagonist to determine the effects of the GABAergic system on LH secretion during reproductive maturation in pre-pubertal Nellore heifers. Nellore heifers (n = 10) were administered a picrotoxin injection of 0.18 mg/kg, i.v. Blood samples were collected every 15 min for 3 h at different developmental stages (8, 10, 14 and 17 mo of age). Plasma concentrations of LH were quantified using an RIA (sensitivity of 0.04 ng/mL and CV of 15 %). There was an interaction between treatment and age (P = 0.034). Picrotoxin-treated heifers had lesser (P <= 0.05) LH mean concentrations during a 3 h period at 10 and 17 mo of age compared to control heifers (P <= 0.05). Comparing the period before and after Picrotoxin injection in the same animals, there was a 33 % decrease in LH concentration at 8 mo of age (P = 0.0165). These results indicate the GABAergic system has a stimulatory function in inducing LH secretion in pre-pubertal Nellore heifers. These findings corroborate previous results that GABA increases GnRH/LH secretion in other species during the pre-pubertal period. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:06:26Z 2021-06-25T15:06:26Z 2021-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2021.106764 Animal Reproduction Science. Amsterdam: Elsevier, v. 229, 6 p., 2021. 0378-4320 http://hdl.handle.net/11449/210374 10.1016/j.anireprosci.2021.106764 WOS:000655469600007 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2021.106764 http://hdl.handle.net/11449/210374 |
identifier_str_mv |
Animal Reproduction Science. Amsterdam: Elsevier, v. 229, 6 p., 2021. 0378-4320 10.1016/j.anireprosci.2021.106764 WOS:000655469600007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964527352086528 |