Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992009000300004 http://hdl.handle.net/11449/225631 |
Resumo: | The immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study was to evaluate the effect of infection with immunosuppression by dexamethasone associated with pentoxifylline on animal weight, spleen weight, spleen and hepatic parasitic load and immunopathology, as well as the IFN-γ and IL-10 production in spleen cell culture of Balb/c mice infected with Leishmania chagasi. The infection did not cause body weight gain in animals, but both the weight and size of the spleen were increased. The immunosuppression using dexamethasone associated with pentoxifylline affected body weight gain and spleen weight and size in both infected and non-infected animals. The immunosuppression did not significantly alter the course of the splenic or hepatic parasite burden. Dexamethasone and pentoxifylline significantly affected cytokine production, but did not influence the Th1/Th2 ratio in infected animals. |
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Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdensCell-mediated responseExperimental infectionImmunosuppressionLeishmania chagaslParasite burdenThe immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study was to evaluate the effect of infection with immunosuppression by dexamethasone associated with pentoxifylline on animal weight, spleen weight, spleen and hepatic parasitic load and immunopathology, as well as the IFN-γ and IL-10 production in spleen cell culture of Balb/c mice infected with Leishmania chagasi. The infection did not cause body weight gain in animals, but both the weight and size of the spleen were increased. The immunosuppression using dexamethasone associated with pentoxifylline affected body weight gain and spleen weight and size in both infected and non-infected animals. The immunosuppression did not significantly alter the course of the splenic or hepatic parasite burden. Dexamethasone and pentoxifylline significantly affected cytokine production, but did not influence the Th1/Th2 ratio in infected animals.Department of Tropical Diseases Botucatu Medical School São Paulo State University, Botucatu, São Paulo StateZoonosis Research Center Veterinary Medicine and Animal Husbandry School São Paulo State University, Botucatu, São Paulo StateDepartment of Microbiology and Immunology Botucatu Biosciences Institute São Paulo State University, Botucatu, São Paulo StateDepartamento de Doenças Tropicais Faculdade de Medicina de Botucatu UNESP, Botucatu, SPDepartment of Tropical Diseases Botucatu Medical School São Paulo State University, Botucatu, São Paulo StateZoonosis Research Center Veterinary Medicine and Animal Husbandry School São Paulo State University, Botucatu, São Paulo StateDepartment of Microbiology and Immunology Botucatu Biosciences Institute São Paulo State University, Botucatu, São Paulo StateDepartamento de Doenças Tropicais Faculdade de Medicina de Botucatu UNESP, Botucatu, SPUniversidade Estadual Paulista (UNESP)Hoffmann, Juliano Leônidas [UNESP]Machado, J. G. [UNESP]Gaio, F. C. [UNESP]Dias-Melicio, L. A. [UNESP]Langoni, H. [UNESP]2022-04-28T20:55:53Z2022-04-28T20:55:53Z2009-10-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article391-410http://dx.doi.org/10.1590/S1678-91992009000300004Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 3, p. 391-410, 2009.1678-9199http://hdl.handle.net/11449/22563110.1590/S1678-919920090003000042-s2.0-70349656689Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T20:55:53Zoai:repositorio.unesp.br:11449/225631Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:55:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
title |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
spellingShingle |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens Hoffmann, Juliano Leônidas [UNESP] Cell-mediated response Experimental infection Immunosuppression Leishmania chagasl Parasite burden |
title_short |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
title_full |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
title_fullStr |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
title_full_unstemmed |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
title_sort |
Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: Cytokines and parasite burdens |
author |
Hoffmann, Juliano Leônidas [UNESP] |
author_facet |
Hoffmann, Juliano Leônidas [UNESP] Machado, J. G. [UNESP] Gaio, F. C. [UNESP] Dias-Melicio, L. A. [UNESP] Langoni, H. [UNESP] |
author_role |
author |
author2 |
Machado, J. G. [UNESP] Gaio, F. C. [UNESP] Dias-Melicio, L. A. [UNESP] Langoni, H. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Hoffmann, Juliano Leônidas [UNESP] Machado, J. G. [UNESP] Gaio, F. C. [UNESP] Dias-Melicio, L. A. [UNESP] Langoni, H. [UNESP] |
dc.subject.por.fl_str_mv |
Cell-mediated response Experimental infection Immunosuppression Leishmania chagasl Parasite burden |
topic |
Cell-mediated response Experimental infection Immunosuppression Leishmania chagasl Parasite burden |
description |
The immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study was to evaluate the effect of infection with immunosuppression by dexamethasone associated with pentoxifylline on animal weight, spleen weight, spleen and hepatic parasitic load and immunopathology, as well as the IFN-γ and IL-10 production in spleen cell culture of Balb/c mice infected with Leishmania chagasi. The infection did not cause body weight gain in animals, but both the weight and size of the spleen were increased. The immunosuppression using dexamethasone associated with pentoxifylline affected body weight gain and spleen weight and size in both infected and non-infected animals. The immunosuppression did not significantly alter the course of the splenic or hepatic parasite burden. Dexamethasone and pentoxifylline significantly affected cytokine production, but did not influence the Th1/Th2 ratio in infected animals. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10-12 2022-04-28T20:55:53Z 2022-04-28T20:55:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992009000300004 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 3, p. 391-410, 2009. 1678-9199 http://hdl.handle.net/11449/225631 10.1590/S1678-91992009000300004 2-s2.0-70349656689 |
url |
http://dx.doi.org/10.1590/S1678-91992009000300004 http://hdl.handle.net/11449/225631 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 3, p. 391-410, 2009. 1678-9199 10.1590/S1678-91992009000300004 2-s2.0-70349656689 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
391-410 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964599011770368 |