Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1472-6793-8-12 http://hdl.handle.net/11449/70482 |
Resumo: | Background. Obesity has been associated with a variety of disease such as type II diabetes mellitus, arterial hypertension and atherosclerosis. Evidences have shown that exercise training promotes beneficial effects on these disorders, but the underlying mechanisms are not fully understood. The aim of this study was to investigate whether physical preconditioning prevents the deleterious effect of high caloric diet in vascular reactivity of rat aortic and mesenteric rings. Methods. Male Wistar rats were divided into sedentary (SD); trained (TR); sedentary diet (SDD) and trained diet (TRD) groups. Run training (RT) was performed in sessions of 60 min, 5 days/week for 12 weeks (70-80% VO2max). Triglycerides, glucose, insulin and nitrite/nitrate concentrations (NOx -) were measured. Concentration- response curves to acetylcholine (ACh) and sodium nitroprusside (SNP) were obtained. Expression of Cu/Zn superoxide dismutase (SOD-1) was assessed by Western blotting. Results. High caloric diet increased triglycerides concentration (SDD: 216 ± 25 mg/dl) and exercise training restored to the baseline value (TRD: 89 ± 9 mg/dl). Physical preconditioning significantly reduced insulin levels in both groups (TR: 0.54 ± 0.1 and TRD: 1.24 ± 0.3 ng/ml) as compared to sedentary animals (SD: 0.87 ± 0.1 and SDD: 2.57 ± 0.3 ng/ml). On the other hand, glucose concentration was slightly increased by high caloric diet, and RT did not modify this parameter (SD: 126 ± 6; TR: 140 ± 8; SDD: 156 ± 8 and TRD 153 ± 9 mg/dl). Neither high caloric diet nor RT modified NO x - levels (SD: 27 ± 4; TR: 28 ± 6; SDD: 27 ± 3 and TRD: 30 ± 2 μM). Functional assays showed that high caloric diet impaired the relaxing response to ACh in mesenteric (about 13%), but not in aortic rings. RT improved the relaxing responses to ACh either in aortic (28%, for TR and 16%, to TRD groups) or mesenteric rings (10%, for TR and 17%, to TRD groups) that was accompanied by up-regulation of SOD-1 expression and reduction in triglycerides levels. Conclusion. The improvement in endothelial function by physical preconditioning in mesenteric and aortic arteries from high caloric fed-rats was directly related to an increase in NO bioavailability to the smooth muscle mostly due to SOD-1 up regulation. © 2008 de Moraes et al; licensee BioMed Central Ltd. |
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Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed ratsacetylcholinecopper zinc superoxide dismutaseglucoseinsulinnitratenitritenitroprusside sodiumtriacylglycerolsuperoxide dismutaseanimal experimentanimal foodaortacaloric intakecontrol groupcontrolled studyexercisefood intakemalemesenterynonhumanratrelaxation trainingWestern blottinganimalfat intakemesenteric arterymetabolismmethodologyphysiologyvascular endotheliumvasodilatationWistar ratAnimalsAortaDietary FatsEndothelium, VascularEnergy IntakeMaleMesenteric ArteriesPhysical Conditioning, AnimalRatsRats, WistarSuperoxide DismutaseVasodilationBackground. Obesity has been associated with a variety of disease such as type II diabetes mellitus, arterial hypertension and atherosclerosis. Evidences have shown that exercise training promotes beneficial effects on these disorders, but the underlying mechanisms are not fully understood. The aim of this study was to investigate whether physical preconditioning prevents the deleterious effect of high caloric diet in vascular reactivity of rat aortic and mesenteric rings. Methods. Male Wistar rats were divided into sedentary (SD); trained (TR); sedentary diet (SDD) and trained diet (TRD) groups. Run training (RT) was performed in sessions of 60 min, 5 days/week for 12 weeks (70-80% VO2max). Triglycerides, glucose, insulin and nitrite/nitrate concentrations (NOx -) were measured. Concentration- response curves to acetylcholine (ACh) and sodium nitroprusside (SNP) were obtained. Expression of Cu/Zn superoxide dismutase (SOD-1) was assessed by Western blotting. Results. High caloric diet increased triglycerides concentration (SDD: 216 ± 25 mg/dl) and exercise training restored to the baseline value (TRD: 89 ± 9 mg/dl). Physical preconditioning significantly reduced insulin levels in both groups (TR: 0.54 ± 0.1 and TRD: 1.24 ± 0.3 ng/ml) as compared to sedentary animals (SD: 0.87 ± 0.1 and SDD: 2.57 ± 0.3 ng/ml). On the other hand, glucose concentration was slightly increased by high caloric diet, and RT did not modify this parameter (SD: 126 ± 6; TR: 140 ± 8; SDD: 156 ± 8 and TRD 153 ± 9 mg/dl). Neither high caloric diet nor RT modified NO x - levels (SD: 27 ± 4; TR: 28 ± 6; SDD: 27 ± 3 and TRD: 30 ± 2 μM). Functional assays showed that high caloric diet impaired the relaxing response to ACh in mesenteric (about 13%), but not in aortic rings. RT improved the relaxing responses to ACh either in aortic (28%, for TR and 16%, to TRD groups) or mesenteric rings (10%, for TR and 17%, to TRD groups) that was accompanied by up-regulation of SOD-1 expression and reduction in triglycerides levels. Conclusion. The improvement in endothelial function by physical preconditioning in mesenteric and aortic arteries from high caloric fed-rats was directly related to an increase in NO bioavailability to the smooth muscle mostly due to SOD-1 up regulation. © 2008 de Moraes et al; licensee BioMed Central Ltd.Biological Science and Health Faculty of Physical Education Cruzeiro Do Sul University, São Paulo (SP)Department of Physiology and Biophysics Institute of Biomedical Sciences (ICB-I) University of São Paulo, São Paulo (SP)Department of Pharmacology Faculty of Medical Sciences University of Campinas, Campinas (SP)Department of Physical Education Institute of Bioscience University of Sao Paulo State, Rio Claro (SP)Universidade Cruzeiro do SulUniversidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Moraes, Camila deDavel, Ana Paula CoutoRossoni, Luciana VenturiniAntunes, EdsonZanesco, Angelina2014-05-27T11:23:36Z2014-05-27T11:23:36Z2008-07-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1472-6793-8-12BMC Physiology, v. 8, n. 1, 2008.1472-6793http://hdl.handle.net/11449/7048210.1186/1472-6793-8-122-s2.0-467491006552-s2.0-46749100655.pdf4472007237545596Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Physiology0,936info:eu-repo/semantics/openAccess2024-01-16T06:29:42Zoai:repositorio.unesp.br:11449/70482Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-16T06:29:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
title |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
spellingShingle |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats Moraes, Camila de acetylcholine copper zinc superoxide dismutase glucose insulin nitrate nitrite nitroprusside sodium triacylglycerol superoxide dismutase animal experiment animal food aorta caloric intake control group controlled study exercise food intake male mesentery nonhuman rat relaxation training Western blotting animal fat intake mesenteric artery metabolism methodology physiology vascular endothelium vasodilatation Wistar rat Animals Aorta Dietary Fats Endothelium, Vascular Energy Intake Male Mesenteric Arteries Physical Conditioning, Animal Rats Rats, Wistar Superoxide Dismutase Vasodilation |
title_short |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
title_full |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
title_fullStr |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
title_full_unstemmed |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
title_sort |
Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats |
author |
Moraes, Camila de |
author_facet |
Moraes, Camila de Davel, Ana Paula Couto Rossoni, Luciana Venturini Antunes, Edson Zanesco, Angelina |
author_role |
author |
author2 |
Davel, Ana Paula Couto Rossoni, Luciana Venturini Antunes, Edson Zanesco, Angelina |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Cruzeiro do Sul Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moraes, Camila de Davel, Ana Paula Couto Rossoni, Luciana Venturini Antunes, Edson Zanesco, Angelina |
dc.subject.por.fl_str_mv |
acetylcholine copper zinc superoxide dismutase glucose insulin nitrate nitrite nitroprusside sodium triacylglycerol superoxide dismutase animal experiment animal food aorta caloric intake control group controlled study exercise food intake male mesentery nonhuman rat relaxation training Western blotting animal fat intake mesenteric artery metabolism methodology physiology vascular endothelium vasodilatation Wistar rat Animals Aorta Dietary Fats Endothelium, Vascular Energy Intake Male Mesenteric Arteries Physical Conditioning, Animal Rats Rats, Wistar Superoxide Dismutase Vasodilation |
topic |
acetylcholine copper zinc superoxide dismutase glucose insulin nitrate nitrite nitroprusside sodium triacylglycerol superoxide dismutase animal experiment animal food aorta caloric intake control group controlled study exercise food intake male mesentery nonhuman rat relaxation training Western blotting animal fat intake mesenteric artery metabolism methodology physiology vascular endothelium vasodilatation Wistar rat Animals Aorta Dietary Fats Endothelium, Vascular Energy Intake Male Mesenteric Arteries Physical Conditioning, Animal Rats Rats, Wistar Superoxide Dismutase Vasodilation |
description |
Background. Obesity has been associated with a variety of disease such as type II diabetes mellitus, arterial hypertension and atherosclerosis. Evidences have shown that exercise training promotes beneficial effects on these disorders, but the underlying mechanisms are not fully understood. The aim of this study was to investigate whether physical preconditioning prevents the deleterious effect of high caloric diet in vascular reactivity of rat aortic and mesenteric rings. Methods. Male Wistar rats were divided into sedentary (SD); trained (TR); sedentary diet (SDD) and trained diet (TRD) groups. Run training (RT) was performed in sessions of 60 min, 5 days/week for 12 weeks (70-80% VO2max). Triglycerides, glucose, insulin and nitrite/nitrate concentrations (NOx -) were measured. Concentration- response curves to acetylcholine (ACh) and sodium nitroprusside (SNP) were obtained. Expression of Cu/Zn superoxide dismutase (SOD-1) was assessed by Western blotting. Results. High caloric diet increased triglycerides concentration (SDD: 216 ± 25 mg/dl) and exercise training restored to the baseline value (TRD: 89 ± 9 mg/dl). Physical preconditioning significantly reduced insulin levels in both groups (TR: 0.54 ± 0.1 and TRD: 1.24 ± 0.3 ng/ml) as compared to sedentary animals (SD: 0.87 ± 0.1 and SDD: 2.57 ± 0.3 ng/ml). On the other hand, glucose concentration was slightly increased by high caloric diet, and RT did not modify this parameter (SD: 126 ± 6; TR: 140 ± 8; SDD: 156 ± 8 and TRD 153 ± 9 mg/dl). Neither high caloric diet nor RT modified NO x - levels (SD: 27 ± 4; TR: 28 ± 6; SDD: 27 ± 3 and TRD: 30 ± 2 μM). Functional assays showed that high caloric diet impaired the relaxing response to ACh in mesenteric (about 13%), but not in aortic rings. RT improved the relaxing responses to ACh either in aortic (28%, for TR and 16%, to TRD groups) or mesenteric rings (10%, for TR and 17%, to TRD groups) that was accompanied by up-regulation of SOD-1 expression and reduction in triglycerides levels. Conclusion. The improvement in endothelial function by physical preconditioning in mesenteric and aortic arteries from high caloric fed-rats was directly related to an increase in NO bioavailability to the smooth muscle mostly due to SOD-1 up regulation. © 2008 de Moraes et al; licensee BioMed Central Ltd. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07-14 2014-05-27T11:23:36Z 2014-05-27T11:23:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1472-6793-8-12 BMC Physiology, v. 8, n. 1, 2008. 1472-6793 http://hdl.handle.net/11449/70482 10.1186/1472-6793-8-12 2-s2.0-46749100655 2-s2.0-46749100655.pdf 4472007237545596 |
url |
http://dx.doi.org/10.1186/1472-6793-8-12 http://hdl.handle.net/11449/70482 |
identifier_str_mv |
BMC Physiology, v. 8, n. 1, 2008. 1472-6793 10.1186/1472-6793-8-12 2-s2.0-46749100655 2-s2.0-46749100655.pdf 4472007237545596 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Physiology 0,936 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965637354717184 |