Effect of injection of L-NAME on drinking response
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X1999001100012 http://hdl.handle.net/11449/28061 |
Resumo: | The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the injection of L-NAME, 10, 20 or 40 µg/µl, into the LV progressively reduced the water intake induced by hypertonic saline, with values of 5.3 ± 0.8, 3.2 ± 0.8 and 0.7 ± 0.01 ml/h, respectively. These results indicate that nitric oxide is involved in the regulation of drinking behavior induced by centrally administered ANG II and cellular dehydration and that the nitric oxide of the SFO plays an important role in this regulation. |
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Repositório Institucional da UNESP |
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spelling |
Effect of injection of L-NAME on drinking responsenitric oxidewater intakeSFOdehydrationCNSThe drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the injection of L-NAME, 10, 20 or 40 µg/µl, into the LV progressively reduced the water intake induced by hypertonic saline, with values of 5.3 ± 0.8, 3.2 ± 0.8 and 0.7 ± 0.01 ml/h, respectively. These results indicate that nitric oxide is involved in the regulation of drinking behavior induced by centrally administered ANG II and cellular dehydration and that the nitric oxide of the SFO plays an important role in this regulation.Universidade Estadual PaulistaUniversidade de TaubatéUniversidade Estadual PaulistaAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Universidade de TaubatéSaad, W.A. [UNESP]Camargo, L.A.A. [UNESP]Saad, R.Pereira, A.F.Simões, S.2014-05-20T15:11:31Z2014-05-20T15:11:31Z1999-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1413-1416application/pdfhttp://dx.doi.org/10.1590/S0100-879X1999001100012Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 11, p. 1413-1416, 1999.0100-879Xhttp://hdl.handle.net/11449/2806110.1590/S0100-879X1999001100012S0100-879X1999001100012S0100-879X1999001100012.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2023-10-31T06:07:40Zoai:repositorio.unesp.br:11449/28061Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-31T06:07:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of injection of L-NAME on drinking response |
title |
Effect of injection of L-NAME on drinking response |
spellingShingle |
Effect of injection of L-NAME on drinking response Saad, W.A. [UNESP] nitric oxide water intake SFO dehydration CNS |
title_short |
Effect of injection of L-NAME on drinking response |
title_full |
Effect of injection of L-NAME on drinking response |
title_fullStr |
Effect of injection of L-NAME on drinking response |
title_full_unstemmed |
Effect of injection of L-NAME on drinking response |
title_sort |
Effect of injection of L-NAME on drinking response |
author |
Saad, W.A. [UNESP] |
author_facet |
Saad, W.A. [UNESP] Camargo, L.A.A. [UNESP] Saad, R. Pereira, A.F. Simões, S. |
author_role |
author |
author2 |
Camargo, L.A.A. [UNESP] Saad, R. Pereira, A.F. Simões, S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Taubaté |
dc.contributor.author.fl_str_mv |
Saad, W.A. [UNESP] Camargo, L.A.A. [UNESP] Saad, R. Pereira, A.F. Simões, S. |
dc.subject.por.fl_str_mv |
nitric oxide water intake SFO dehydration CNS |
topic |
nitric oxide water intake SFO dehydration CNS |
description |
The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the injection of L-NAME, 10, 20 or 40 µg/µl, into the LV progressively reduced the water intake induced by hypertonic saline, with values of 5.3 ± 0.8, 3.2 ± 0.8 and 0.7 ± 0.01 ml/h, respectively. These results indicate that nitric oxide is involved in the regulation of drinking behavior induced by centrally administered ANG II and cellular dehydration and that the nitric oxide of the SFO plays an important role in this regulation. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-11-01 2014-05-20T15:11:31Z 2014-05-20T15:11:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X1999001100012 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 11, p. 1413-1416, 1999. 0100-879X http://hdl.handle.net/11449/28061 10.1590/S0100-879X1999001100012 S0100-879X1999001100012 S0100-879X1999001100012.pdf |
url |
http://dx.doi.org/10.1590/S0100-879X1999001100012 http://hdl.handle.net/11449/28061 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 11, p. 1413-1416, 1999. 0100-879X 10.1590/S0100-879X1999001100012 S0100-879X1999001100012 S0100-879X1999001100012.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research 1.492 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1413-1416 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964765526687744 |