Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.electrochemsci.org/papers/vol8/80607795.pdf http://hdl.handle.net/11449/75595 |
Resumo: | An enzymatic biosensor based on inhibition of acetylcholinesterase activity was developed to analyze methomyl contamination in different matrices. The detection limits under optimal working conditions were found to be 30.4 μg L-1 methomyl using the biosensor and 0.15 μg L-1 using HPLC. The biosensor was then applied to analysis of methomyl in fruit and vegetable samples at concentrations in the ppb range in the absence of sample pretreatment. Recovery levels using the biosensor were effective, ranging from 78.0 to 96.5%, while the HPLC method yielded 57.0 to 99.5% recovery. Results for total carbamate concentrations obtained using the acetylcholinesterase biosensor were compared to those obtained using HPLC. © 2013 by ESG. |
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Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensorBiosensorFood analysisMethomylPesticidesAn enzymatic biosensor based on inhibition of acetylcholinesterase activity was developed to analyze methomyl contamination in different matrices. The detection limits under optimal working conditions were found to be 30.4 μg L-1 methomyl using the biosensor and 0.15 μg L-1 using HPLC. The biosensor was then applied to analysis of methomyl in fruit and vegetable samples at concentrations in the ppb range in the absence of sample pretreatment. Recovery levels using the biosensor were effective, ranging from 78.0 to 96.5%, while the HPLC method yielded 57.0 to 99.5% recovery. Results for total carbamate concentrations obtained using the acetylcholinesterase biosensor were compared to those obtained using HPLC. © 2013 by ESG.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual do Oeste do Parań, 85903-000 - Toledo - ParanáUniversidade Paranaense UNIPAR, Umuarama- PRInstitute of Bioscience Department of Chemistry and Biochemistry UNESP, Botucatu, SPInstituto de Química de São Carlos Universidade de São Paulo, 13560-970 São Carlos - SPInstitute of Bioscience Department of Chemistry and Biochemistry UNESP, Botucatu, SPFAPESP: 03/07653-8FAPESP: 06/51625-7Universidade Estadual do Oeste do ParańUNIPARUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Caetano, JosianeDragunski, Douglas C.Pedrosa, Valber A. [UNESP]Machado, Sergio A.S.2014-05-27T11:29:38Z2014-05-27T11:29:38Z2013-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7795-7805application/pdfhttp://www.electrochemsci.org/papers/vol8/80607795.pdfInternational Journal of Electrochemical Science, v. 8, n. 6, p. 7795-7805, 2013.1452-3981http://hdl.handle.net/11449/75595WOS:0003235461000262-s2.0-848789930012-s2.0-84878993001.pdf7781282422851911Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Electrochemical Science1.3690,366info:eu-repo/semantics/openAccess2023-12-26T06:15:57Zoai:repositorio.unesp.br:11449/75595Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-26T06:15:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
title |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
spellingShingle |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor Caetano, Josiane Biosensor Food analysis Methomyl Pesticides |
title_short |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
title_full |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
title_fullStr |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
title_full_unstemmed |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
title_sort |
Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor |
author |
Caetano, Josiane |
author_facet |
Caetano, Josiane Dragunski, Douglas C. Pedrosa, Valber A. [UNESP] Machado, Sergio A.S. |
author_role |
author |
author2 |
Dragunski, Douglas C. Pedrosa, Valber A. [UNESP] Machado, Sergio A.S. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual do Oeste do Parań UNIPAR Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Caetano, Josiane Dragunski, Douglas C. Pedrosa, Valber A. [UNESP] Machado, Sergio A.S. |
dc.subject.por.fl_str_mv |
Biosensor Food analysis Methomyl Pesticides |
topic |
Biosensor Food analysis Methomyl Pesticides |
description |
An enzymatic biosensor based on inhibition of acetylcholinesterase activity was developed to analyze methomyl contamination in different matrices. The detection limits under optimal working conditions were found to be 30.4 μg L-1 methomyl using the biosensor and 0.15 μg L-1 using HPLC. The biosensor was then applied to analysis of methomyl in fruit and vegetable samples at concentrations in the ppb range in the absence of sample pretreatment. Recovery levels using the biosensor were effective, ranging from 78.0 to 96.5%, while the HPLC method yielded 57.0 to 99.5% recovery. Results for total carbamate concentrations obtained using the acetylcholinesterase biosensor were compared to those obtained using HPLC. © 2013 by ESG. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 2014-05-27T11:29:38Z 2014-05-27T11:29:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.electrochemsci.org/papers/vol8/80607795.pdf International Journal of Electrochemical Science, v. 8, n. 6, p. 7795-7805, 2013. 1452-3981 http://hdl.handle.net/11449/75595 WOS:000323546100026 2-s2.0-84878993001 2-s2.0-84878993001.pdf 7781282422851911 |
url |
http://www.electrochemsci.org/papers/vol8/80607795.pdf http://hdl.handle.net/11449/75595 |
identifier_str_mv |
International Journal of Electrochemical Science, v. 8, n. 6, p. 7795-7805, 2013. 1452-3981 WOS:000323546100026 2-s2.0-84878993001 2-s2.0-84878993001.pdf 7781282422851911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Electrochemical Science 1.369 0,366 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7795-7805 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790111798132736 |