Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.foodres.2023.112731 http://hdl.handle.net/11449/247095 |
Resumo: | The aim of this study was to characterize the proteins differentially expressed in the pectoralis major muscle of broilers supplemented with passion fruit seed oil (PFSO) under cyclic heat stress conditions. Ninety one-day-old male chicks were housed in cages arranged in a climatic chamber, where they were kept under cyclic heat stress for eight hours a day from the beginning to the end of the experiment. The birds were divided into two experimental groups, one group supplemented with 0.9% PFSO and a control group (CON) without PFSO supplementation. At 36 days of age, 18 birds were slaughtered to collect muscle samples. From pools of breast fillet samples from each group, proteolytic cleavage of the protein extracts was performed, and later, the peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The 0.9% PFSO supplementation revealed the modulation of 57 proteins in the pectoralis major muscle of broilers exposed to cyclic heat stress. Among them, four proteins were upregulated, and 46 proteins were downregulated. In addition, seven proteins were expressed only in the CON group. These results suggest that PFSO may increase heat tolerance, with a possible reduction in oxidative stress, activation of neuroprotective mechanisms, protection against apoptosis, decrease in inflammatory responses, and regulation of energy metabolism. |
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Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditionsGel-freeLC-MS/MSOxidative stressThe aim of this study was to characterize the proteins differentially expressed in the pectoralis major muscle of broilers supplemented with passion fruit seed oil (PFSO) under cyclic heat stress conditions. Ninety one-day-old male chicks were housed in cages arranged in a climatic chamber, where they were kept under cyclic heat stress for eight hours a day from the beginning to the end of the experiment. The birds were divided into two experimental groups, one group supplemented with 0.9% PFSO and a control group (CON) without PFSO supplementation. At 36 days of age, 18 birds were slaughtered to collect muscle samples. From pools of breast fillet samples from each group, proteolytic cleavage of the protein extracts was performed, and later, the peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The 0.9% PFSO supplementation revealed the modulation of 57 proteins in the pectoralis major muscle of broilers exposed to cyclic heat stress. Among them, four proteins were upregulated, and 46 proteins were downregulated. In addition, seven proteins were expressed only in the CON group. These results suggest that PFSO may increase heat tolerance, with a possible reduction in oxidative stress, activation of neuroprotective mechanisms, protection against apoptosis, decrease in inflammatory responses, and regulation of energy metabolism.School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), São PauloInstitute of Biosciences São Paulo State University (UNESP), São PauloUniversity of São Paulo (USP)School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), São PauloInstitute of Biosciences São Paulo State University (UNESP), São PauloUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Sávio de Almeida Assunção, Andrey [UNESP]Aparecida Martins, Renata [UNESP]Cavalcante Souza Vieira, José [UNESP]Campos Rocha, Leone [UNESP]Kaiser de Lima Krenchinski, Fernanda [UNESP]Afonso Rabelo Buzalaf, MaríliaRoberto Sartori, José [UNESP]de Magalhães Padilha, Pedro [UNESP]2023-07-29T13:06:09Z2023-07-29T13:06:09Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.foodres.2023.112731Food Research International, v. 167.1873-71450963-9969http://hdl.handle.net/11449/24709510.1016/j.foodres.2023.1127312-s2.0-85151404343Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Research Internationalinfo:eu-repo/semantics/openAccess2023-07-29T13:06:10Zoai:repositorio.unesp.br:11449/247095Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:06:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
title |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
spellingShingle |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions Sávio de Almeida Assunção, Andrey [UNESP] Gel-free LC-MS/MS Oxidative stress |
title_short |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
title_full |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
title_fullStr |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
title_full_unstemmed |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
title_sort |
Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions |
author |
Sávio de Almeida Assunção, Andrey [UNESP] |
author_facet |
Sávio de Almeida Assunção, Andrey [UNESP] Aparecida Martins, Renata [UNESP] Cavalcante Souza Vieira, José [UNESP] Campos Rocha, Leone [UNESP] Kaiser de Lima Krenchinski, Fernanda [UNESP] Afonso Rabelo Buzalaf, Marília Roberto Sartori, José [UNESP] de Magalhães Padilha, Pedro [UNESP] |
author_role |
author |
author2 |
Aparecida Martins, Renata [UNESP] Cavalcante Souza Vieira, José [UNESP] Campos Rocha, Leone [UNESP] Kaiser de Lima Krenchinski, Fernanda [UNESP] Afonso Rabelo Buzalaf, Marília Roberto Sartori, José [UNESP] de Magalhães Padilha, Pedro [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Sávio de Almeida Assunção, Andrey [UNESP] Aparecida Martins, Renata [UNESP] Cavalcante Souza Vieira, José [UNESP] Campos Rocha, Leone [UNESP] Kaiser de Lima Krenchinski, Fernanda [UNESP] Afonso Rabelo Buzalaf, Marília Roberto Sartori, José [UNESP] de Magalhães Padilha, Pedro [UNESP] |
dc.subject.por.fl_str_mv |
Gel-free LC-MS/MS Oxidative stress |
topic |
Gel-free LC-MS/MS Oxidative stress |
description |
The aim of this study was to characterize the proteins differentially expressed in the pectoralis major muscle of broilers supplemented with passion fruit seed oil (PFSO) under cyclic heat stress conditions. Ninety one-day-old male chicks were housed in cages arranged in a climatic chamber, where they were kept under cyclic heat stress for eight hours a day from the beginning to the end of the experiment. The birds were divided into two experimental groups, one group supplemented with 0.9% PFSO and a control group (CON) without PFSO supplementation. At 36 days of age, 18 birds were slaughtered to collect muscle samples. From pools of breast fillet samples from each group, proteolytic cleavage of the protein extracts was performed, and later, the peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The 0.9% PFSO supplementation revealed the modulation of 57 proteins in the pectoralis major muscle of broilers exposed to cyclic heat stress. Among them, four proteins were upregulated, and 46 proteins were downregulated. In addition, seven proteins were expressed only in the CON group. These results suggest that PFSO may increase heat tolerance, with a possible reduction in oxidative stress, activation of neuroprotective mechanisms, protection against apoptosis, decrease in inflammatory responses, and regulation of energy metabolism. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:06:09Z 2023-07-29T13:06:09Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.foodres.2023.112731 Food Research International, v. 167. 1873-7145 0963-9969 http://hdl.handle.net/11449/247095 10.1016/j.foodres.2023.112731 2-s2.0-85151404343 |
url |
http://dx.doi.org/10.1016/j.foodres.2023.112731 http://hdl.handle.net/11449/247095 |
identifier_str_mv |
Food Research International, v. 167. 1873-7145 0963-9969 10.1016/j.foodres.2023.112731 2-s2.0-85151404343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Research International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964416865730560 |