Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envpol.2018.02.030 http://hdl.handle.net/11449/179610 |
Resumo: | Benzo[a]pyrene (BaP) is a bio-accumulative toxic compound found in the atmosphere, water, and soil that may affect the life cycle of amphibians. In this study, a few contamination biomarkers, such as hepatic melanomacrophages (MMs), mast cells, erythrocyte micronuclei (MN) and white blood cells were used to determine how BaP acts in these cells in the anurans Physalaemus cuvieri and Leptodactylus fuscus. Animals of both species were divided into three treatment groups: 1 day, 7 days and 13 days, subcutaneously injected 2 mg/kg BaP diluted in mineral oil and control group with only mineral oil. After 7 days, BaP caused the frequency of MN to increase in both species while reducing melanin area. The micronucleus frequency increased due to the genotoxicity of BaP, while the decreasing melanin area may be related to the inhibition of tyrosinase activity, an enzyme responsible for regulating melanogenesis, decreasing the synthesis of melanin. The mast cell density increased in all groups and in both species as a response to the inflammatory action of BaP. These cells respond to nonspecific inflammatory effects leading, therefore, to this response in all treatments. The percentage of leukocytes remained unchanged probably due to great intraspecific variability. Additionally, the leukocyte profiles of both species were characterized and the differences were attributed to extrinsic factors. In short, BaP can affect the integrity of several organs and tissues, and cell functions leading to the conclusion that this compound is hepatotoxic, genotoxic and immunotoxic for anurans. BaP is harmful for anurans, since it influences the response of important immune cells such as melanomacrophages, mast cells and leukocytes, besides inducing micronucleus formation in erythrocytes. |
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Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)AnuranErythrocytesLeukocytesLiver melanomacrophageMicronucleiBenzo[a]pyrene (BaP) is a bio-accumulative toxic compound found in the atmosphere, water, and soil that may affect the life cycle of amphibians. In this study, a few contamination biomarkers, such as hepatic melanomacrophages (MMs), mast cells, erythrocyte micronuclei (MN) and white blood cells were used to determine how BaP acts in these cells in the anurans Physalaemus cuvieri and Leptodactylus fuscus. Animals of both species were divided into three treatment groups: 1 day, 7 days and 13 days, subcutaneously injected 2 mg/kg BaP diluted in mineral oil and control group with only mineral oil. After 7 days, BaP caused the frequency of MN to increase in both species while reducing melanin area. The micronucleus frequency increased due to the genotoxicity of BaP, while the decreasing melanin area may be related to the inhibition of tyrosinase activity, an enzyme responsible for regulating melanogenesis, decreasing the synthesis of melanin. The mast cell density increased in all groups and in both species as a response to the inflammatory action of BaP. These cells respond to nonspecific inflammatory effects leading, therefore, to this response in all treatments. The percentage of leukocytes remained unchanged probably due to great intraspecific variability. Additionally, the leukocyte profiles of both species were characterized and the differences were attributed to extrinsic factors. In short, BaP can affect the integrity of several organs and tissues, and cell functions leading to the conclusion that this compound is hepatotoxic, genotoxic and immunotoxic for anurans. BaP is harmful for anurans, since it influences the response of important immune cells such as melanomacrophages, mast cells and leukocytes, besides inducing micronucleus formation in erythrocytes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Graduate Program in Animal Biology Universidade Estadual Paulista (UNESP)Department of Biology Universidade Estadual Paulista (UNESP)Graduate Program in Biotechnology and Environmental monitoring CCTS Federal University of São CarlosGraduate Program in Animal Biology Universidade Estadual Paulista (UNESP)Department of Biology Universidade Estadual Paulista (UNESP)FAPESP: 2014/07466-8CNPq: 305081/2015-2Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Fanali, Lara Zácari [UNESP]Franco-Belussi, Lilian [UNESP]Bonini-Domingos, Cláudia Regina [UNESP]de Oliveira, Classius [UNESP]2018-12-11T17:36:01Z2018-12-11T17:36:01Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article93-102application/pdfhttp://dx.doi.org/10.1016/j.envpol.2018.02.030Environmental Pollution, v. 237, p. 93-102.1873-64240269-7491http://hdl.handle.net/11449/17961010.1016/j.envpol.2018.02.0302-s2.0-850423880522-s2.0-85042388052.pdf32794280661767190000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Pollution1,6151,615info:eu-repo/semantics/openAccess2023-11-16T06:12:02Zoai:repositorio.unesp.br:11449/179610Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-16T06:12:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
title |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
spellingShingle |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) Fanali, Lara Zácari [UNESP] Anuran Erythrocytes Leukocytes Liver melanomacrophage Micronuclei |
title_short |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
title_full |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
title_fullStr |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
title_full_unstemmed |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
title_sort |
Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae) |
author |
Fanali, Lara Zácari [UNESP] |
author_facet |
Fanali, Lara Zácari [UNESP] Franco-Belussi, Lilian [UNESP] Bonini-Domingos, Cláudia Regina [UNESP] de Oliveira, Classius [UNESP] |
author_role |
author |
author2 |
Franco-Belussi, Lilian [UNESP] Bonini-Domingos, Cláudia Regina [UNESP] de Oliveira, Classius [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Fanali, Lara Zácari [UNESP] Franco-Belussi, Lilian [UNESP] Bonini-Domingos, Cláudia Regina [UNESP] de Oliveira, Classius [UNESP] |
dc.subject.por.fl_str_mv |
Anuran Erythrocytes Leukocytes Liver melanomacrophage Micronuclei |
topic |
Anuran Erythrocytes Leukocytes Liver melanomacrophage Micronuclei |
description |
Benzo[a]pyrene (BaP) is a bio-accumulative toxic compound found in the atmosphere, water, and soil that may affect the life cycle of amphibians. In this study, a few contamination biomarkers, such as hepatic melanomacrophages (MMs), mast cells, erythrocyte micronuclei (MN) and white blood cells were used to determine how BaP acts in these cells in the anurans Physalaemus cuvieri and Leptodactylus fuscus. Animals of both species were divided into three treatment groups: 1 day, 7 days and 13 days, subcutaneously injected 2 mg/kg BaP diluted in mineral oil and control group with only mineral oil. After 7 days, BaP caused the frequency of MN to increase in both species while reducing melanin area. The micronucleus frequency increased due to the genotoxicity of BaP, while the decreasing melanin area may be related to the inhibition of tyrosinase activity, an enzyme responsible for regulating melanogenesis, decreasing the synthesis of melanin. The mast cell density increased in all groups and in both species as a response to the inflammatory action of BaP. These cells respond to nonspecific inflammatory effects leading, therefore, to this response in all treatments. The percentage of leukocytes remained unchanged probably due to great intraspecific variability. Additionally, the leukocyte profiles of both species were characterized and the differences were attributed to extrinsic factors. In short, BaP can affect the integrity of several organs and tissues, and cell functions leading to the conclusion that this compound is hepatotoxic, genotoxic and immunotoxic for anurans. BaP is harmful for anurans, since it influences the response of important immune cells such as melanomacrophages, mast cells and leukocytes, besides inducing micronucleus formation in erythrocytes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:36:01Z 2018-12-11T17:36:01Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envpol.2018.02.030 Environmental Pollution, v. 237, p. 93-102. 1873-6424 0269-7491 http://hdl.handle.net/11449/179610 10.1016/j.envpol.2018.02.030 2-s2.0-85042388052 2-s2.0-85042388052.pdf 3279428066176719 0000-0002-4603-9467 |
url |
http://dx.doi.org/10.1016/j.envpol.2018.02.030 http://hdl.handle.net/11449/179610 |
identifier_str_mv |
Environmental Pollution, v. 237, p. 93-102. 1873-6424 0269-7491 10.1016/j.envpol.2018.02.030 2-s2.0-85042388052 2-s2.0-85042388052.pdf 3279428066176719 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Pollution 1,615 1,615 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
93-102 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964957106765824 |