Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jdent.2022.104237 http://hdl.handle.net/11449/242084 |
Resumo: | Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces. |
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Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradationCollagenDentinHydroxyprolineMMPPre-reacted glass fillerProteolysisObjectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Genetics Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)Department of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)Department of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)Department of Dentistry Maringá State University (UEM), ParanáDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Genetics Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)Department of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)Department of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)CNPq: 138984/2020–4FAPESP: 2019/16473–1CNPq: 303391/2019–7Universidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)Mendes Soares, Igor Paulino [UNESP]Anselmi, Caroline [UNESP]Guiné, Isabela [UNESP]Fernandes, Lídia de Oliveira [UNESP]Pires, Maria Luiza Barucci Araujo [UNESP]de Souza Costa, Carlos Alberto [UNESP]Scheffel, Débora Lopes SallesHebling, Josimeri [UNESP]2023-03-02T08:37:44Z2023-03-02T08:37:44Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jdent.2022.104237Journal of Dentistry, v. 124.0300-5712http://hdl.handle.net/11449/24208410.1016/j.jdent.2022.1042372-s2.0-85134944888Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dentistryinfo:eu-repo/semantics/openAccess2023-03-02T08:37:44Zoai:repositorio.unesp.br:11449/242084Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T08:37:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
title |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
spellingShingle |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation Mendes Soares, Igor Paulino [UNESP] Collagen Dentin Hydroxyproline MMP Pre-reacted glass filler Proteolysis |
title_short |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
title_full |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
title_fullStr |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
title_full_unstemmed |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
title_sort |
Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation |
author |
Mendes Soares, Igor Paulino [UNESP] |
author_facet |
Mendes Soares, Igor Paulino [UNESP] Anselmi, Caroline [UNESP] Guiné, Isabela [UNESP] Fernandes, Lídia de Oliveira [UNESP] Pires, Maria Luiza Barucci Araujo [UNESP] de Souza Costa, Carlos Alberto [UNESP] Scheffel, Débora Lopes Salles Hebling, Josimeri [UNESP] |
author_role |
author |
author2 |
Anselmi, Caroline [UNESP] Guiné, Isabela [UNESP] Fernandes, Lídia de Oliveira [UNESP] Pires, Maria Luiza Barucci Araujo [UNESP] de Souza Costa, Carlos Alberto [UNESP] Scheffel, Débora Lopes Salles Hebling, Josimeri [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Maringá (UEM) |
dc.contributor.author.fl_str_mv |
Mendes Soares, Igor Paulino [UNESP] Anselmi, Caroline [UNESP] Guiné, Isabela [UNESP] Fernandes, Lídia de Oliveira [UNESP] Pires, Maria Luiza Barucci Araujo [UNESP] de Souza Costa, Carlos Alberto [UNESP] Scheffel, Débora Lopes Salles Hebling, Josimeri [UNESP] |
dc.subject.por.fl_str_mv |
Collagen Dentin Hydroxyproline MMP Pre-reacted glass filler Proteolysis |
topic |
Collagen Dentin Hydroxyproline MMP Pre-reacted glass filler Proteolysis |
description |
Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-02T08:37:44Z 2023-03-02T08:37:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jdent.2022.104237 Journal of Dentistry, v. 124. 0300-5712 http://hdl.handle.net/11449/242084 10.1016/j.jdent.2022.104237 2-s2.0-85134944888 |
url |
http://dx.doi.org/10.1016/j.jdent.2022.104237 http://hdl.handle.net/11449/242084 |
identifier_str_mv |
Journal of Dentistry, v. 124. 0300-5712 10.1016/j.jdent.2022.104237 2-s2.0-85134944888 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964602459488256 |