Proteolytic activity and degradation of bovine versus human dentin matrices
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/194740 |
Resumo: | Non-human teeth have been commonly used in research as replacements for human teeth, and potential dissimilarities between the dental tissues should be considered when interpreting the outcomes. Objective: To compare the proteolytic activity and degradation rate of bovine and human dentin matrices. Methodology: Dentin beam specimens were obtained from human molars (n=30) and bovine incisors (n=30). The beams were weighed hydrated and after complete dehydration to obtain the mineralized wet and dry masses. Then, the beams were demineralized in 10 wt% phosphoric acid. Next, 15 beams from each substrate were randomly selected and again dehydrated and weighed to obtain the initial demineralized dry mass (DM). Then, the beams were stored in saliva-like buffer solution (SLBS) for 7, 14 and 21 days. SLBS was used to evaluate hydroxyproline (HYP) release after each storage period. The remaining beams of each substrate (n=15) were tested for initial MMP activity using a colorimetric assay and then also stored in SLBS. DM and MMP activity were reassessed after 7, 14 and 21 days of incubation. The data were subjected to two-way ANOVA tests with repeated measures complemented by Bonferroni’s tests. Unpaired two-tailed t-tests were also used (p<0.05). Results: Similar water and inorganic fractions were found in human and bovine dentin, while human dentin had a higher protein content. The most intense proteolytic activity and matrix deterioration occurred short after dentin was demineralized. Both substrates exhibited a sharp reduction in MMP activity after seven days of incubation. Although human dentin had higher MMP activity levels, greater HYP release and DM loss after seven days than bovine dentin, after 14 and 21 days, the outcomes were not statistically different. Conclusion: Bovine dentin is a suitable substrate for long-term studies involving the degradation of dentin matrices. |
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oai:revistas.usp.br:article/194740 |
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Journal of applied oral science (Online) |
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Proteolytic activity and degradation of bovine versus human dentin matricesDentinAnimalsCollagenMatrix metalloproteinasesHydroxyprolineNon-human teeth have been commonly used in research as replacements for human teeth, and potential dissimilarities between the dental tissues should be considered when interpreting the outcomes. Objective: To compare the proteolytic activity and degradation rate of bovine and human dentin matrices. Methodology: Dentin beam specimens were obtained from human molars (n=30) and bovine incisors (n=30). The beams were weighed hydrated and after complete dehydration to obtain the mineralized wet and dry masses. Then, the beams were demineralized in 10 wt% phosphoric acid. Next, 15 beams from each substrate were randomly selected and again dehydrated and weighed to obtain the initial demineralized dry mass (DM). Then, the beams were stored in saliva-like buffer solution (SLBS) for 7, 14 and 21 days. SLBS was used to evaluate hydroxyproline (HYP) release after each storage period. The remaining beams of each substrate (n=15) were tested for initial MMP activity using a colorimetric assay and then also stored in SLBS. DM and MMP activity were reassessed after 7, 14 and 21 days of incubation. The data were subjected to two-way ANOVA tests with repeated measures complemented by Bonferroni’s tests. Unpaired two-tailed t-tests were also used (p<0.05). Results: Similar water and inorganic fractions were found in human and bovine dentin, while human dentin had a higher protein content. The most intense proteolytic activity and matrix deterioration occurred short after dentin was demineralized. Both substrates exhibited a sharp reduction in MMP activity after seven days of incubation. Although human dentin had higher MMP activity levels, greater HYP release and DM loss after seven days than bovine dentin, after 14 and 21 days, the outcomes were not statistically different. Conclusion: Bovine dentin is a suitable substrate for long-term studies involving the degradation of dentin matrices.Universidade de São Paulo. Faculdade de Odontologia de Bauru2022-02-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19474010.1590/1678-7757-2021-0290Journal of Applied Oral Science; Vol. 29 (2021); e20210290Journal of Applied Oral Science; Vol. 29 (2021); e20210290Journal of Applied Oral Science; v. 29 (2021); e202102901678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/194740/179940Copyright (c) 2022 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessInagati, Cristiane Mayumi Scheffel, Débora Lopes Salles Anovazzi, Giovana Alonso, Juliana Rosa Luiz Christoffoli , Marcelly Tupan Pashley , David Henry Costa, Carlos Alberto De Souza Hebling , Josimeri 2022-02-08T13:30:43Zoai:revistas.usp.br:article/194740Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2022-02-08T13:30:43Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Proteolytic activity and degradation of bovine versus human dentin matrices |
title |
Proteolytic activity and degradation of bovine versus human dentin matrices |
spellingShingle |
Proteolytic activity and degradation of bovine versus human dentin matrices Inagati, Cristiane Mayumi Dentin Animals Collagen Matrix metalloproteinases Hydroxyproline |
title_short |
Proteolytic activity and degradation of bovine versus human dentin matrices |
title_full |
Proteolytic activity and degradation of bovine versus human dentin matrices |
title_fullStr |
Proteolytic activity and degradation of bovine versus human dentin matrices |
title_full_unstemmed |
Proteolytic activity and degradation of bovine versus human dentin matrices |
title_sort |
Proteolytic activity and degradation of bovine versus human dentin matrices |
author |
Inagati, Cristiane Mayumi |
author_facet |
Inagati, Cristiane Mayumi Scheffel, Débora Lopes Salles Anovazzi, Giovana Alonso, Juliana Rosa Luiz Christoffoli , Marcelly Tupan Pashley , David Henry Costa, Carlos Alberto De Souza Hebling , Josimeri |
author_role |
author |
author2 |
Scheffel, Débora Lopes Salles Anovazzi, Giovana Alonso, Juliana Rosa Luiz Christoffoli , Marcelly Tupan Pashley , David Henry Costa, Carlos Alberto De Souza Hebling , Josimeri |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Inagati, Cristiane Mayumi Scheffel, Débora Lopes Salles Anovazzi, Giovana Alonso, Juliana Rosa Luiz Christoffoli , Marcelly Tupan Pashley , David Henry Costa, Carlos Alberto De Souza Hebling , Josimeri |
dc.subject.por.fl_str_mv |
Dentin Animals Collagen Matrix metalloproteinases Hydroxyproline |
topic |
Dentin Animals Collagen Matrix metalloproteinases Hydroxyproline |
description |
Non-human teeth have been commonly used in research as replacements for human teeth, and potential dissimilarities between the dental tissues should be considered when interpreting the outcomes. Objective: To compare the proteolytic activity and degradation rate of bovine and human dentin matrices. Methodology: Dentin beam specimens were obtained from human molars (n=30) and bovine incisors (n=30). The beams were weighed hydrated and after complete dehydration to obtain the mineralized wet and dry masses. Then, the beams were demineralized in 10 wt% phosphoric acid. Next, 15 beams from each substrate were randomly selected and again dehydrated and weighed to obtain the initial demineralized dry mass (DM). Then, the beams were stored in saliva-like buffer solution (SLBS) for 7, 14 and 21 days. SLBS was used to evaluate hydroxyproline (HYP) release after each storage period. The remaining beams of each substrate (n=15) were tested for initial MMP activity using a colorimetric assay and then also stored in SLBS. DM and MMP activity were reassessed after 7, 14 and 21 days of incubation. The data were subjected to two-way ANOVA tests with repeated measures complemented by Bonferroni’s tests. Unpaired two-tailed t-tests were also used (p<0.05). Results: Similar water and inorganic fractions were found in human and bovine dentin, while human dentin had a higher protein content. The most intense proteolytic activity and matrix deterioration occurred short after dentin was demineralized. Both substrates exhibited a sharp reduction in MMP activity after seven days of incubation. Although human dentin had higher MMP activity levels, greater HYP release and DM loss after seven days than bovine dentin, after 14 and 21 days, the outcomes were not statistically different. Conclusion: Bovine dentin is a suitable substrate for long-term studies involving the degradation of dentin matrices. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/194740 10.1590/1678-7757-2021-0290 |
url |
https://www.revistas.usp.br/jaos/article/view/194740 |
identifier_str_mv |
10.1590/1678-7757-2021-0290 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/194740/179940 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 29 (2021); e20210290 Journal of Applied Oral Science; Vol. 29 (2021); e20210290 Journal of Applied Oral Science; v. 29 (2021); e20210290 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1787713196939804672 |