Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

Detalhes bibliográficos
Autor(a) principal: Guimarães, Andresa
Data de Publicação: 2019
Outros Autores: Raimundo, Juliana Macedo, Rodrigues, Raisa Braul, Peixoto, Maristela Peckle, Santos, Huarrisson Azevedo, André, Marcos Rogério [UNESP], Machado, Rosangela Zacarias [UNESP], Baldani, Cristiane Divan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/s1984-296120180088
http://hdl.handle.net/11449/188934
Resumo: Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.
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spelling Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast BrazilInfecção de Ehrlichia spp. em gatos domésticos da Grande Rio de Janeiro, sudeste do BrasilEhrlichiosisFelineHematologyIFANested PCREhrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.Departamento de Medicina e Cirurgia Veterinária Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro - UFRRJUniversidade Federal Fluminense - UFFDepartamento de Epidemiologia e Saúde Pública Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro - UFRRJDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias - FCAV Universidade Estadual Paulista - UNESPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias - FCAV Universidade Estadual Paulista - UNESPUniversidade Federal Rural do Rio de Janeiro - UFRRJUniversidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Guimarães, AndresaRaimundo, Juliana MacedoRodrigues, Raisa BraulPeixoto, Maristela PeckleSantos, Huarrisson AzevedoAndré, Marcos Rogério [UNESP]Machado, Rosangela Zacarias [UNESP]Baldani, Cristiane Divan2019-10-06T16:23:55Z2019-10-06T16:23:55Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article180-185application/pdfhttp://dx.doi.org/10.1590/s1984-296120180088Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 1, p. 180-185, 2019.0103-846Xhttp://hdl.handle.net/11449/18893410.1590/s1984-296120180088S1984-296120190001001802-s2.0-85063923138S1984-29612019000100180.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-12-23T06:24:11Zoai:repositorio.unesp.br:11449/188934Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-23T06:24:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
Infecção de Ehrlichia spp. em gatos domésticos da Grande Rio de Janeiro, sudeste do Brasil
title Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
spellingShingle Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
Guimarães, Andresa
Ehrlichiosis
Feline
Hematology
IFA
Nested PCR
title_short Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
title_full Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
title_fullStr Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
title_full_unstemmed Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
title_sort Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
author Guimarães, Andresa
author_facet Guimarães, Andresa
Raimundo, Juliana Macedo
Rodrigues, Raisa Braul
Peixoto, Maristela Peckle
Santos, Huarrisson Azevedo
André, Marcos Rogério [UNESP]
Machado, Rosangela Zacarias [UNESP]
Baldani, Cristiane Divan
author_role author
author2 Raimundo, Juliana Macedo
Rodrigues, Raisa Braul
Peixoto, Maristela Peckle
Santos, Huarrisson Azevedo
André, Marcos Rogério [UNESP]
Machado, Rosangela Zacarias [UNESP]
Baldani, Cristiane Divan
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro - UFRRJ
Universidade Federal Fluminense (UFF)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Guimarães, Andresa
Raimundo, Juliana Macedo
Rodrigues, Raisa Braul
Peixoto, Maristela Peckle
Santos, Huarrisson Azevedo
André, Marcos Rogério [UNESP]
Machado, Rosangela Zacarias [UNESP]
Baldani, Cristiane Divan
dc.subject.por.fl_str_mv Ehrlichiosis
Feline
Hematology
IFA
Nested PCR
topic Ehrlichiosis
Feline
Hematology
IFA
Nested PCR
description Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:23:55Z
2019-10-06T16:23:55Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/s1984-296120180088
Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 1, p. 180-185, 2019.
0103-846X
http://hdl.handle.net/11449/188934
10.1590/s1984-296120180088
S1984-29612019000100180
2-s2.0-85063923138
S1984-29612019000100180.pdf
9139899895580513
url http://dx.doi.org/10.1590/s1984-296120180088
http://hdl.handle.net/11449/188934
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 1, p. 180-185, 2019.
0103-846X
10.1590/s1984-296120180088
S1984-29612019000100180
2-s2.0-85063923138
S1984-29612019000100180.pdf
9139899895580513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 180-185
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797790090579148800

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