Bactérias oportunistas e genes associados às β- lactamases de amplo espectro na boca de pacientes oncológicos submetidos à radioterapia de cabeça e pescoço

• Autor(a) principal: Okimoto, Anderson Teruo [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156614; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000900361.pdf
• topic: Radioterapia; Neoplasias de cabeça e pescoço; Mucosite; beta-Lactamases; Radiotherapy

The present study evaluated the occurrence of members of the family Enterobacteriaceae and of the genus Pseudomonas, as well as the main resistance markers that codify for the broad-spectrum β-lactamases in the supra and subgingival biofilm, besides the oral mucosa of 50 cancer patients submitted to head and neck radiotherapy (RT). After intraoral and extraoral clinical examinations with evaluation of dental and periodontal conditions, clinical specimens of supra and subgingival biofilm as well as mucosa scraping of these patients were collected immediately prior to radiotherapy treatment, 15-22 days after the start of radiotherapy, immediately after the end of RT and 6 months after RT. The presence of target microorganisms and resistance markers was determined by amplification of the target DNA by PCR using primers and specific amplification conditions. The results were submitted to the Chi-square test, Mann-Whitney test and Fisher's exact test, while interrelationships between selected microorganisms resistance markers and the clinical characteristics of the patients were evaluated using the Spearman correlation test. There was a progressive increase in the distribution of the target microorganisms throughout the RT, especially in the patients who presented severe mucositis. The presence of the blaTEM gene showed a correlation with the presence of the Enterobacteriaceae family in the specimens collected and also increased with RT