Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats

• Autor(a) principal: de Lemos Neto, Sylvio Valença [UNESP]
• Data de Publicação: 2017
• Outros Autores: Vianna, Isabela Galvão [UNESP], Castiglia, Yara Marcondes Machado [UNESP], Golim, Marjorie de Assis [UNESP], de Souza, Aparecida Vitória Gonçalves [UNESP], de Carvalho, Lídia Raquel [UNESP], Deffune, Elenice [UNESP], do Nascimento Junior, Paulo [UNESP], Módolo, Norma Sueli Pinheiro [UNESP], Vianna, Pedro Thadeu Galvão [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-865020170030000004; http://hdl.handle.net/11449/174445
• topic: Apoptosis; Cyclosporine; Hyperglycemia; Kidney; Rats; Reperfusion injury

Purpose: To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats. Methods: In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells. Results: Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury. Conclusion: In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.