Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11274-022-03494-z http://hdl.handle.net/11449/246524 |
Resumo: | The present study aims to evaluate the growth potential of the P. aeruginosa ATCC9027 strain with molasses as the sole carbon source to produce rhamnolipids. The influence of the cultivation time and substrate concentration on biosurfactant production was investigated by using a complete 3-level factorial design, with the rhamnolipid concentration as the variable response. The strain was able to produce the biosurfactant in all design conditions tested, producing 758.04 mg/L rhamnolipids with 7% v/v substrate concentration in a cultivation time of 120 h. The substrate concentration used in the cultivation step directly influenced the biosurfactant production, and, even with the decrease in biomass growth, the biosurfactant production continued to increase. High Performance Liquid Chromatography (HPLC) revealed the presence of 62.3% mono- (RL1) and 37.6% di-rhamnolipids (RL3). The stability tests showed that the biosurfactant has good performance in extreme conditions of temperature, pH and saline concentration. The emulsification index was also evaluated for several oils and hydrocarbons, obtaining emulsification rates of up to 84.9% for the burnt motor oil. In addition, rhamnolipid showed a good ability to remove spilled oil from the sand, removing 58.51% of burnt motor oil and 70.09% of post-frying soybean oil. The results indicate that molasses, an agro-industrial residue abundant in Brazil, can be used as the only carbon source for quality rhamnolipid production when under optimized conditions, therefore presenting itself as a management option for this residue and, at the same time, providing the production product with high added value. Graphical abstract: [Figure not available: see fulltext.] |
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Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrateAgro-industrial subproductBiosurfactantMolassesPseudomonas aeruginosaRhamnolipidsThe present study aims to evaluate the growth potential of the P. aeruginosa ATCC9027 strain with molasses as the sole carbon source to produce rhamnolipids. The influence of the cultivation time and substrate concentration on biosurfactant production was investigated by using a complete 3-level factorial design, with the rhamnolipid concentration as the variable response. The strain was able to produce the biosurfactant in all design conditions tested, producing 758.04 mg/L rhamnolipids with 7% v/v substrate concentration in a cultivation time of 120 h. The substrate concentration used in the cultivation step directly influenced the biosurfactant production, and, even with the decrease in biomass growth, the biosurfactant production continued to increase. High Performance Liquid Chromatography (HPLC) revealed the presence of 62.3% mono- (RL1) and 37.6% di-rhamnolipids (RL3). The stability tests showed that the biosurfactant has good performance in extreme conditions of temperature, pH and saline concentration. The emulsification index was also evaluated for several oils and hydrocarbons, obtaining emulsification rates of up to 84.9% for the burnt motor oil. In addition, rhamnolipid showed a good ability to remove spilled oil from the sand, removing 58.51% of burnt motor oil and 70.09% of post-frying soybean oil. The results indicate that molasses, an agro-industrial residue abundant in Brazil, can be used as the only carbon source for quality rhamnolipid production when under optimized conditions, therefore presenting itself as a management option for this residue and, at the same time, providing the production product with high added value. Graphical abstract: [Figure not available: see fulltext.]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)State University of Campinas, Av. Albert Einstein, 500, Cidade Universitária Zeferino Vaz, SPInstitute of Biosciences São Paulo State University (Unesp), SPFederal University of Alfenas, Rodovia José Aurélio Vilela, 11999 (BR 267 Km 533) Cidade Universitária, MGInstitute of Biosciences São Paulo State University (Unesp), SPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Federal University of AlfenasBraz, Letícia MartiniSalazar-Bryam, Ana María [UNESP]Andrade, Grazielle Santos SilvaTambourgi, Elias Basille2023-07-29T12:43:23Z2023-07-29T12:43:23Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11274-022-03494-zWorld Journal of Microbiology and Biotechnology, v. 39, n. 2, 2023.1573-09720959-3993http://hdl.handle.net/11449/24652410.1007/s11274-022-03494-z2-s2.0-85144593507Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Microbiology and Biotechnologyinfo:eu-repo/semantics/openAccess2023-07-29T12:43:23Zoai:repositorio.unesp.br:11449/246524Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:43:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
title |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
spellingShingle |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate Braz, Letícia Martini Agro-industrial subproduct Biosurfactant Molasses Pseudomonas aeruginosa Rhamnolipids |
title_short |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
title_full |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
title_fullStr |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
title_full_unstemmed |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
title_sort |
Optimization and characterization of rhamnolipids produced by Pseudomonas aeruginosa ATCC 9027 using molasses as a substrate |
author |
Braz, Letícia Martini |
author_facet |
Braz, Letícia Martini Salazar-Bryam, Ana María [UNESP] Andrade, Grazielle Santos Silva Tambourgi, Elias Basille |
author_role |
author |
author2 |
Salazar-Bryam, Ana María [UNESP] Andrade, Grazielle Santos Silva Tambourgi, Elias Basille |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Federal University of Alfenas |
dc.contributor.author.fl_str_mv |
Braz, Letícia Martini Salazar-Bryam, Ana María [UNESP] Andrade, Grazielle Santos Silva Tambourgi, Elias Basille |
dc.subject.por.fl_str_mv |
Agro-industrial subproduct Biosurfactant Molasses Pseudomonas aeruginosa Rhamnolipids |
topic |
Agro-industrial subproduct Biosurfactant Molasses Pseudomonas aeruginosa Rhamnolipids |
description |
The present study aims to evaluate the growth potential of the P. aeruginosa ATCC9027 strain with molasses as the sole carbon source to produce rhamnolipids. The influence of the cultivation time and substrate concentration on biosurfactant production was investigated by using a complete 3-level factorial design, with the rhamnolipid concentration as the variable response. The strain was able to produce the biosurfactant in all design conditions tested, producing 758.04 mg/L rhamnolipids with 7% v/v substrate concentration in a cultivation time of 120 h. The substrate concentration used in the cultivation step directly influenced the biosurfactant production, and, even with the decrease in biomass growth, the biosurfactant production continued to increase. High Performance Liquid Chromatography (HPLC) revealed the presence of 62.3% mono- (RL1) and 37.6% di-rhamnolipids (RL3). The stability tests showed that the biosurfactant has good performance in extreme conditions of temperature, pH and saline concentration. The emulsification index was also evaluated for several oils and hydrocarbons, obtaining emulsification rates of up to 84.9% for the burnt motor oil. In addition, rhamnolipid showed a good ability to remove spilled oil from the sand, removing 58.51% of burnt motor oil and 70.09% of post-frying soybean oil. The results indicate that molasses, an agro-industrial residue abundant in Brazil, can be used as the only carbon source for quality rhamnolipid production when under optimized conditions, therefore presenting itself as a management option for this residue and, at the same time, providing the production product with high added value. Graphical abstract: [Figure not available: see fulltext.] |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:43:23Z 2023-07-29T12:43:23Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11274-022-03494-z World Journal of Microbiology and Biotechnology, v. 39, n. 2, 2023. 1573-0972 0959-3993 http://hdl.handle.net/11449/246524 10.1007/s11274-022-03494-z 2-s2.0-85144593507 |
url |
http://dx.doi.org/10.1007/s11274-022-03494-z http://hdl.handle.net/11449/246524 |
identifier_str_mv |
World Journal of Microbiology and Biotechnology, v. 39, n. 2, 2023. 1573-0972 0959-3993 10.1007/s11274-022-03494-z 2-s2.0-85144593507 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World Journal of Microbiology and Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790379959910400 |