Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2013/670451 http://hdl.handle.net/11449/75436 |
Resumo: | Since the beginning of propolis research, several groups have studied its antibacterial, antifungal, and antiviral properties. However, most of these studies have only employed propolis ethanolic extract (PEE) leading to little knowledge about the biological activities of propolis water extract (PWE). Based on this, in a previous study, we demonstrated the anti-inflammatory and immunomodulatory activities of PWE. In order to better understand the equilibrium between effectiveness and toxicity, which is essential for a new medicine, the characteristics of PWE were analyzed. We developed and validated an RP-HPLC method to chemically characterize PWE and PEE and evaluated the in vitro antioxidant/antimicrobial activity for both extracts and the safety of PWE via determining genotoxic potential using in vitro and in vivo mammalian micronucleus assays. We have concluded that the proposed analytical methodology was reliable, and both extracts showed similar chemical composition. The extracts presented antioxidant and antimicrobial effects, while PWE demonstrated higher antioxidant activity and more efficacious for the most of the microorganisms tested than PEE. Finally, PWE was shown to be safe using micronucleus assays. © 2013 Bruno Alves Rocha et al. |
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Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisationpropolisanimal experimentantimicrobial activityantioxidant activitycontrolled studydrug efficacydrug safetydrug screeninggenotoxicityin vitro studyin vivo studymalemicronucleus testnonhumanpriority journalratreversed phase high performance liquid chromatographySince the beginning of propolis research, several groups have studied its antibacterial, antifungal, and antiviral properties. However, most of these studies have only employed propolis ethanolic extract (PEE) leading to little knowledge about the biological activities of propolis water extract (PWE). Based on this, in a previous study, we demonstrated the anti-inflammatory and immunomodulatory activities of PWE. In order to better understand the equilibrium between effectiveness and toxicity, which is essential for a new medicine, the characteristics of PWE were analyzed. We developed and validated an RP-HPLC method to chemically characterize PWE and PEE and evaluated the in vitro antioxidant/antimicrobial activity for both extracts and the safety of PWE via determining genotoxic potential using in vitro and in vivo mammalian micronucleus assays. We have concluded that the proposed analytical methodology was reliable, and both extracts showed similar chemical composition. The extracts presented antioxidant and antimicrobial effects, while PWE demonstrated higher antioxidant activity and more efficacious for the most of the microorganisms tested than PEE. Finally, PWE was shown to be safe using micronucleus assays. © 2013 Bruno Alves Rocha et al.Laboratório de Pesquisa, Desenvolvimento e Inovação (P, D and I) Apis Flora Industrial e Comercial LTDA, Rua Triunfo, 945, 14020-670 Ribeirão Preto, SPDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, Avenida Bandeirantes, 3900, 14040-901 Ribeirão Preto, SPInstituto de Química Universidade Estadual Paulista, Rua Francisco Degni, 55, 14800-900 Araraquara, SPDepartamento de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, SPUniversidade de Franca Parque Universitário, Avenida Dr. Armando Salles de Oliveira, 201, 14404-600 Franca, SPInstituto de Química Universidade Estadual Paulista, Rua Francisco Degni, 55, 14800-900 Araraquara, SPApis Flora Industrial e Comercial LTDAUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Parque UniversitárioRocha, Bruno AlvesBueno, Paula Carolina Pires [UNESP]Vaz, Mirela Mara de Oliveira Lima LeiteNascimento, Andresa PiacezziFerreira, Nathália UrsoliMoreno, Gabriela de PaduaRodrigues, Marina RezendeCosta-Machado, Ana Rita de MelloBarizon, Edna AparecidaCampos, Jacqueline Costa LimaDe Oliveira, Pollyanna FrancielliAcésio, Nathália de OliveiraMartins, Sabrina de Paula LimaTavares, Denise CrispimBerretta, Andresa Aparecida2014-05-27T11:29:32Z2014-05-27T11:29:32Z2013-05-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2013/670451Evidence-based Complementary and Alternative Medicine, v. 2013.1741-427X1741-4288http://hdl.handle.net/11449/7543610.1155/2013/670451WOS:0003187148000012-s2.0-848779571942-s2.0-84877957194.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.0640,683info:eu-repo/semantics/openAccess2023-12-10T06:15:50Zoai:repositorio.unesp.br:11449/75436Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-10T06:15:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
title |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
spellingShingle |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation Rocha, Bruno Alves propolis animal experiment antimicrobial activity antioxidant activity controlled study drug efficacy drug safety drug screening genotoxicity in vitro study in vivo study male micronucleus test nonhuman priority journal rat reversed phase high performance liquid chromatography |
title_short |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
title_full |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
title_fullStr |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
title_full_unstemmed |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
title_sort |
Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation |
author |
Rocha, Bruno Alves |
author_facet |
Rocha, Bruno Alves Bueno, Paula Carolina Pires [UNESP] Vaz, Mirela Mara de Oliveira Lima Leite Nascimento, Andresa Piacezzi Ferreira, Nathália Ursoli Moreno, Gabriela de Padua Rodrigues, Marina Rezende Costa-Machado, Ana Rita de Mello Barizon, Edna Aparecida Campos, Jacqueline Costa Lima De Oliveira, Pollyanna Francielli Acésio, Nathália de Oliveira Martins, Sabrina de Paula Lima Tavares, Denise Crispim Berretta, Andresa Aparecida |
author_role |
author |
author2 |
Bueno, Paula Carolina Pires [UNESP] Vaz, Mirela Mara de Oliveira Lima Leite Nascimento, Andresa Piacezzi Ferreira, Nathália Ursoli Moreno, Gabriela de Padua Rodrigues, Marina Rezende Costa-Machado, Ana Rita de Mello Barizon, Edna Aparecida Campos, Jacqueline Costa Lima De Oliveira, Pollyanna Francielli Acésio, Nathália de Oliveira Martins, Sabrina de Paula Lima Tavares, Denise Crispim Berretta, Andresa Aparecida |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Apis Flora Industrial e Comercial LTDA Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Parque Universitário |
dc.contributor.author.fl_str_mv |
Rocha, Bruno Alves Bueno, Paula Carolina Pires [UNESP] Vaz, Mirela Mara de Oliveira Lima Leite Nascimento, Andresa Piacezzi Ferreira, Nathália Ursoli Moreno, Gabriela de Padua Rodrigues, Marina Rezende Costa-Machado, Ana Rita de Mello Barizon, Edna Aparecida Campos, Jacqueline Costa Lima De Oliveira, Pollyanna Francielli Acésio, Nathália de Oliveira Martins, Sabrina de Paula Lima Tavares, Denise Crispim Berretta, Andresa Aparecida |
dc.subject.por.fl_str_mv |
propolis animal experiment antimicrobial activity antioxidant activity controlled study drug efficacy drug safety drug screening genotoxicity in vitro study in vivo study male micronucleus test nonhuman priority journal rat reversed phase high performance liquid chromatography |
topic |
propolis animal experiment antimicrobial activity antioxidant activity controlled study drug efficacy drug safety drug screening genotoxicity in vitro study in vivo study male micronucleus test nonhuman priority journal rat reversed phase high performance liquid chromatography |
description |
Since the beginning of propolis research, several groups have studied its antibacterial, antifungal, and antiviral properties. However, most of these studies have only employed propolis ethanolic extract (PEE) leading to little knowledge about the biological activities of propolis water extract (PWE). Based on this, in a previous study, we demonstrated the anti-inflammatory and immunomodulatory activities of PWE. In order to better understand the equilibrium between effectiveness and toxicity, which is essential for a new medicine, the characteristics of PWE were analyzed. We developed and validated an RP-HPLC method to chemically characterize PWE and PEE and evaluated the in vitro antioxidant/antimicrobial activity for both extracts and the safety of PWE via determining genotoxic potential using in vitro and in vivo mammalian micronucleus assays. We have concluded that the proposed analytical methodology was reliable, and both extracts showed similar chemical composition. The extracts presented antioxidant and antimicrobial effects, while PWE demonstrated higher antioxidant activity and more efficacious for the most of the microorganisms tested than PEE. Finally, PWE was shown to be safe using micronucleus assays. © 2013 Bruno Alves Rocha et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-27 2014-05-27T11:29:32Z 2014-05-27T11:29:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2013/670451 Evidence-based Complementary and Alternative Medicine, v. 2013. 1741-427X 1741-4288 http://hdl.handle.net/11449/75436 10.1155/2013/670451 WOS:000318714800001 2-s2.0-84877957194 2-s2.0-84877957194.pdf |
url |
http://dx.doi.org/10.1155/2013/670451 http://hdl.handle.net/11449/75436 |
identifier_str_mv |
Evidence-based Complementary and Alternative Medicine, v. 2013. 1741-427X 1741-4288 10.1155/2013/670451 WOS:000318714800001 2-s2.0-84877957194 2-s2.0-84877957194.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine 2.064 0,683 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965231822143488 |