Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10493-019-00368-2 http://hdl.handle.net/11449/190334 |
Resumo: | The cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efficacy of 99.2% (LC50 = 18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efficient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50 = 3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent effect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50 > 4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the field. |
id |
UNSP_214749b94686d8002d70b8bc8b3c938a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/190334 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)AcaricidesLivestockMicroscopyNatural productsSpotted feverTicksThe cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efficacy of 99.2% (LC50 = 18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efficient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50 = 3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent effect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50 > 4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the field.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Instituto de Química de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Museu de Zoologia Universidade Estadual de Campinas (UNICAMP)Instituto de Biociências de Rio Claro Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Empresa Brasileira de Pesquisa Agropecuária Embrapa Pecuária SudesteInstituto de Química de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Instituto de Biociências de Rio Claro Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)CNPq: 150302/2017-7FAPESP: 2013/03493-8FAPESP: 2013/07600-3/CIBFarUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Politi, Flávio Augusto Sanches [UNESP]Fantatto, Rafaela Regina [UNESP]da Silva, Alexander Alves [UNESP]Moro, Isabela Jacob [UNESP]Sampieri, Bruno RodriguesCamargo-Mathias, Maria Izabel [UNESP]Figueiredo, Amandade Souza Chagas, Ana CarolinaFurlan, Maysa [UNESP]2019-10-06T17:09:49Z2019-10-06T17:09:49Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article601-618http://dx.doi.org/10.1007/s10493-019-00368-2Experimental and Applied Acarology, v. 77, n. 4, p. 601-618, 2019.1572-97020168-8162http://hdl.handle.net/11449/19033410.1007/s10493-019-00368-22-s2.0-850657353691308042794786872Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental and Applied Acarologyinfo:eu-repo/semantics/openAccess2021-10-23T19:23:43Zoai:repositorio.unesp.br:11449/190334Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
spellingShingle |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Politi, Flávio Augusto Sanches [UNESP] Acaricides Livestock Microscopy Natural products Spotted fever Ticks |
title_short |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_full |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_fullStr |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_full_unstemmed |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_sort |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
author |
Politi, Flávio Augusto Sanches [UNESP] |
author_facet |
Politi, Flávio Augusto Sanches [UNESP] Fantatto, Rafaela Regina [UNESP] da Silva, Alexander Alves [UNESP] Moro, Isabela Jacob [UNESP] Sampieri, Bruno Rodrigues Camargo-Mathias, Maria Izabel [UNESP] Figueiredo, Amanda de Souza Chagas, Ana Carolina Furlan, Maysa [UNESP] |
author_role |
author |
author2 |
Fantatto, Rafaela Regina [UNESP] da Silva, Alexander Alves [UNESP] Moro, Isabela Jacob [UNESP] Sampieri, Bruno Rodrigues Camargo-Mathias, Maria Izabel [UNESP] Figueiredo, Amanda de Souza Chagas, Ana Carolina Furlan, Maysa [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Politi, Flávio Augusto Sanches [UNESP] Fantatto, Rafaela Regina [UNESP] da Silva, Alexander Alves [UNESP] Moro, Isabela Jacob [UNESP] Sampieri, Bruno Rodrigues Camargo-Mathias, Maria Izabel [UNESP] Figueiredo, Amanda de Souza Chagas, Ana Carolina Furlan, Maysa [UNESP] |
dc.subject.por.fl_str_mv |
Acaricides Livestock Microscopy Natural products Spotted fever Ticks |
topic |
Acaricides Livestock Microscopy Natural products Spotted fever Ticks |
description |
The cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efficacy of 99.2% (LC50 = 18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efficient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50 = 3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent effect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50 > 4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the field. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:09:49Z 2019-10-06T17:09:49Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10493-019-00368-2 Experimental and Applied Acarology, v. 77, n. 4, p. 601-618, 2019. 1572-9702 0168-8162 http://hdl.handle.net/11449/190334 10.1007/s10493-019-00368-2 2-s2.0-85065735369 1308042794786872 |
url |
http://dx.doi.org/10.1007/s10493-019-00368-2 http://hdl.handle.net/11449/190334 |
identifier_str_mv |
Experimental and Applied Acarology, v. 77, n. 4, p. 601-618, 2019. 1572-9702 0168-8162 10.1007/s10493-019-00368-2 2-s2.0-85065735369 1308042794786872 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental and Applied Acarology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
601-618 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961549157007360 |