Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation
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Publication Date: | 2016 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1007/s11270-016-3101-y http://hdl.handle.net/11449/173889 |
Summary: | Filamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The isolates were submitted to biodegradability test using 2.6-dichlorophenol indophenol (DCPIP), gallic acid reaction, and evaluation of BaP influence in the enzymatic (ligninolytic) activity. The selected fungi were submitted to the taxonomic identification and used in biodegradation assays, which were carried out using gas chromatography coupled to mass spectrometry (GC-MS). A qualitative analysis of the presence of BaP metabolites that have recently been reported in literature was also performed by GC-MS. A total of 146 fungal isolates were recovered. Among them, 63.7 % were positive for the redox indicator DCPIP. From these isolates, 22.6 % showed positive responses to the gallic acid reaction. In enzymatic tests, the fungi Megasporoporia sp. S47 and unidentified Sordariales S69 presented highest activities of laccase and manganese peroxidase in the presence of BaP. Additionally, the white-rot fungus Megasporoporia sp. S47 showed better performance in BaP degradation (54 %). Therefore, Megasporoporia sp. S47, obtained from an environment with considerable PAH contamination, was selected as a promising genetic resource for application in new studies related to enzyme production and characterization and BaP degradation optimization. |
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Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene DegradationBenzo(a)pyreneBiodegradationFungiLigninolytic enzymesPolycyclic aromatic hydrocarbonsFilamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The isolates were submitted to biodegradability test using 2.6-dichlorophenol indophenol (DCPIP), gallic acid reaction, and evaluation of BaP influence in the enzymatic (ligninolytic) activity. The selected fungi were submitted to the taxonomic identification and used in biodegradation assays, which were carried out using gas chromatography coupled to mass spectrometry (GC-MS). A qualitative analysis of the presence of BaP metabolites that have recently been reported in literature was also performed by GC-MS. A total of 146 fungal isolates were recovered. Among them, 63.7 % were positive for the redox indicator DCPIP. From these isolates, 22.6 % showed positive responses to the gallic acid reaction. In enzymatic tests, the fungi Megasporoporia sp. S47 and unidentified Sordariales S69 presented highest activities of laccase and manganese peroxidase in the presence of BaP. Additionally, the white-rot fungus Megasporoporia sp. S47 showed better performance in BaP degradation (54 %). Therefore, Megasporoporia sp. S47, obtained from an environment with considerable PAH contamination, was selected as a promising genetic resource for application in new studies related to enzyme production and characterization and BaP degradation optimization.Universidade do Estado do Amazonas Escola Superior de Ciências da Saúde, Av. Carvalho Leal, 1777Universidade Estadual Paulista “Júlio de Mesquita Filho” Campus de Rio Claro Departamento deBioquímica e Microbiologia Aplicada IB/UNESP, Av. 24-a, 1515Universidade Federal do Amazonas Departamento de Ciências Fundamentais e DesenvolvimentoAgrícola (DCFDA) / Faculdade de Ciências Agrárias (FCA) e Centro de Apoio Multidisciplinar Divisão deBiotecnologia, Av. General Rodrigo Octávio Jordão Ramos, 3000Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2.936Universidade Estadual Paulista “Júlio de Mesquita Filho” Campus de Rio Claro Departamento deBioquímica e Microbiologia Aplicada IB/UNESP, Av. 24-a, 1515Escola Superior de Ciências da SaúdeUniversidade Estadual Paulista (Unesp)Divisão deBiotecnologiaInstituto Nacional de Pesquisas da Amazônia (INPA)de Lima Souza, Hilton MarceloSette, Lara Durães [UNESP]da Mota, Adolfo Josédo Nascimento Neto, Joaquim FerreiraRodrigues, André [UNESP]de Oliveira, Tássio Brito [UNESP]de Oliveira, Fernando Mendesde Oliveira, Luiz Antôniodos Santos Barroso, HileiaZanotto, Sandra Patricia2018-12-11T17:08:12Z2018-12-11T17:08:12Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1007/s11270-016-3101-yWater, Air, and Soil Pollution, v. 227, n. 12, 2016.1573-29320049-6979http://hdl.handle.net/11449/17388910.1007/s11270-016-3101-y2-s2.0-850035542962-s2.0-85003554296.pdf5969653098289575Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWater, Air, and Soil Pollution0,5890,589info:eu-repo/semantics/openAccess2023-11-23T06:10:06Zoai:repositorio.unesp.br:11449/173889Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:10:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
title |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
spellingShingle |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation de Lima Souza, Hilton Marcelo Benzo(a)pyrene Biodegradation Fungi Ligninolytic enzymes Polycyclic aromatic hydrocarbons |
title_short |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
title_full |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
title_fullStr |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
title_full_unstemmed |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
title_sort |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
author |
de Lima Souza, Hilton Marcelo |
author_facet |
de Lima Souza, Hilton Marcelo Sette, Lara Durães [UNESP] da Mota, Adolfo José do Nascimento Neto, Joaquim Ferreira Rodrigues, André [UNESP] de Oliveira, Tássio Brito [UNESP] de Oliveira, Fernando Mendes de Oliveira, Luiz Antônio dos Santos Barroso, Hileia Zanotto, Sandra Patricia |
author_role |
author |
author2 |
Sette, Lara Durães [UNESP] da Mota, Adolfo José do Nascimento Neto, Joaquim Ferreira Rodrigues, André [UNESP] de Oliveira, Tássio Brito [UNESP] de Oliveira, Fernando Mendes de Oliveira, Luiz Antônio dos Santos Barroso, Hileia Zanotto, Sandra Patricia |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Escola Superior de Ciências da Saúde Universidade Estadual Paulista (Unesp) Divisão deBiotecnologia Instituto Nacional de Pesquisas da Amazônia (INPA) |
dc.contributor.author.fl_str_mv |
de Lima Souza, Hilton Marcelo Sette, Lara Durães [UNESP] da Mota, Adolfo José do Nascimento Neto, Joaquim Ferreira Rodrigues, André [UNESP] de Oliveira, Tássio Brito [UNESP] de Oliveira, Fernando Mendes de Oliveira, Luiz Antônio dos Santos Barroso, Hileia Zanotto, Sandra Patricia |
dc.subject.por.fl_str_mv |
Benzo(a)pyrene Biodegradation Fungi Ligninolytic enzymes Polycyclic aromatic hydrocarbons |
topic |
Benzo(a)pyrene Biodegradation Fungi Ligninolytic enzymes Polycyclic aromatic hydrocarbons |
description |
Filamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The isolates were submitted to biodegradability test using 2.6-dichlorophenol indophenol (DCPIP), gallic acid reaction, and evaluation of BaP influence in the enzymatic (ligninolytic) activity. The selected fungi were submitted to the taxonomic identification and used in biodegradation assays, which were carried out using gas chromatography coupled to mass spectrometry (GC-MS). A qualitative analysis of the presence of BaP metabolites that have recently been reported in literature was also performed by GC-MS. A total of 146 fungal isolates were recovered. Among them, 63.7 % were positive for the redox indicator DCPIP. From these isolates, 22.6 % showed positive responses to the gallic acid reaction. In enzymatic tests, the fungi Megasporoporia sp. S47 and unidentified Sordariales S69 presented highest activities of laccase and manganese peroxidase in the presence of BaP. Additionally, the white-rot fungus Megasporoporia sp. S47 showed better performance in BaP degradation (54 %). Therefore, Megasporoporia sp. S47, obtained from an environment with considerable PAH contamination, was selected as a promising genetic resource for application in new studies related to enzyme production and characterization and BaP degradation optimization. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 2018-12-11T17:08:12Z 2018-12-11T17:08:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11270-016-3101-y Water, Air, and Soil Pollution, v. 227, n. 12, 2016. 1573-2932 0049-6979 http://hdl.handle.net/11449/173889 10.1007/s11270-016-3101-y 2-s2.0-85003554296 2-s2.0-85003554296.pdf 5969653098289575 |
url |
http://dx.doi.org/10.1007/s11270-016-3101-y http://hdl.handle.net/11449/173889 |
identifier_str_mv |
Water, Air, and Soil Pollution, v. 227, n. 12, 2016. 1573-2932 0049-6979 10.1007/s11270-016-3101-y 2-s2.0-85003554296 2-s2.0-85003554296.pdf 5969653098289575 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Water, Air, and Soil Pollution 0,589 0,589 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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