Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0034-8910.2014048005224 http://hdl.handle.net/11449/114108 |
Resumo: | OBJECTIVEThis study investigated the serological status of dogs living in a visceral leishmaniasis-endemic area and its correlation with the parasitological condition of the animals. METHODSCanine humoral response was evaluated using the sera of 134 dogs by enzyme-linked immunosorbent assay and immunohistochemistry to detect parasites in the skin, lymph node, and spleen of the animals. The specific antibodies investigated were IgG, IgG1, IgG2, and IgE. RESULTSAccording to the parasitological, laboratory, and clinical findings, the dogs were placed into one of four groups: asymptomatic with (AP+, n = 21) or without (AP-, n = 36) Leishmania tissue parasitism and symptomatic with (SP+, n = 52) or without (SP-, n = 25) parasitism. Higher IgG and IgE levels were positively correlated with the infection condition and parasite load, but not with the clinical status. In all groups, total IgG was the predominant antibody, which occurred at the expense of IgG2 instead of IgG1. Most of the infected dogs tested positive for IgG (SP+, 98.1%; AP+, 95.2%), whereas this was not observed with IgE (SP+, 80.8%; AP+, 71.2%). The most relevant finding was the high positivity of the uninfected dogs for Leishmania-specific IgG (SP-, 60.0%; AP-, 44.4%), IgE (SP-, 44.0%; AP-, 27.8%), IgG1 (SP-, 28.0%; AP-, 22.2%), and IgG2 antibodies (SP-, 56.0%; AP-, 41.7%). CONCLUSIONSThe serological status of dogs, as determined by any class or subclass of antibodies, did not accurately distinguish dogs infected with L. (L.) infantum chagasi from uninfected animals. The inaccuracy of the serological result may impair not only the diagnosis, but also epidemiological investigations and strategies for visceral leishmaniasis control. This complex serological scenario occurring in a visceral leishmaniasis-endemic area highlights the challenges associated with canine diagnosis and points out the difficulties experienced by veterinary clinicians and coordinators of control programs. |
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Repositório Institucional da UNESP |
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Serological and infection statuses of dogs from a visceral leishmaniasis-endemic areaStatus sorológico e de infecção canina em área endêmica de leishmaniose visceralLeishmaniose Visceral, diagnósticoLeishmaniose Visceral, epidemiologiaLeishmania, imunologiaCãesEstudos SoroepidemiológicosDoenças Endêmicas, veterináriaLeishmaniasisVisceraldiagnosisepidemiologyLeishmaniaimmunologyDogsSeroepidemiologic StudiesEndemic DiseasesveterinaryOBJECTIVEThis study investigated the serological status of dogs living in a visceral leishmaniasis-endemic area and its correlation with the parasitological condition of the animals. METHODSCanine humoral response was evaluated using the sera of 134 dogs by enzyme-linked immunosorbent assay and immunohistochemistry to detect parasites in the skin, lymph node, and spleen of the animals. The specific antibodies investigated were IgG, IgG1, IgG2, and IgE. RESULTSAccording to the parasitological, laboratory, and clinical findings, the dogs were placed into one of four groups: asymptomatic with (AP+, n = 21) or without (AP-, n = 36) Leishmania tissue parasitism and symptomatic with (SP+, n = 52) or without (SP-, n = 25) parasitism. Higher IgG and IgE levels were positively correlated with the infection condition and parasite load, but not with the clinical status. In all groups, total IgG was the predominant antibody, which occurred at the expense of IgG2 instead of IgG1. Most of the infected dogs tested positive for IgG (SP+, 98.1%; AP+, 95.2%), whereas this was not observed with IgE (SP+, 80.8%; AP+, 71.2%). The most relevant finding was the high positivity of the uninfected dogs for Leishmania-specific IgG (SP-, 60.0%; AP-, 44.4%), IgE (SP-, 44.0%; AP-, 27.8%), IgG1 (SP-, 28.0%; AP-, 22.2%), and IgG2 antibodies (SP-, 56.0%; AP-, 41.7%). CONCLUSIONSThe serological status of dogs, as determined by any class or subclass of antibodies, did not accurately distinguish dogs infected with L. (L.) infantum chagasi from uninfected animals. The inaccuracy of the serological result may impair not only the diagnosis, but also epidemiological investigations and strategies for visceral leishmaniasis control. This complex serological scenario occurring in a visceral leishmaniasis-endemic area highlights the challenges associated with canine diagnosis and points out the difficulties experienced by veterinary clinicians and coordinators of control programs.OBJETIVO Foi investigado o status sorológico de cães, em área endêmica de leishmaniose visceral, e sua correlação com a infecção parasitológica dos animais. MÉTODOS A resposta humoral canina foi avaliada no soro de 134 cães pelo método ELISA e pela imuno-histoquímica, para detectar parasitos na pele, linfonodo e baço desses animais. Os anticorpos específicos investigados foram IgG, IgG1, IgG2 e IgE. RESULTADOS De acordo com os achados parasitológicos, laboratoriais e clínicos, os cães foram alocados em um dos quatro grupos: assintomáticos com (AP+, n = 21) e sem (AP-, n = 36) parasitismo tecidual por Leishmania e sintomáticos com (SP+, n = 52) ou sem (SP-, n = 25) parasitismo. Níveis mais elevados de IgG e IgE se correlacionaram positivamente com o status de infecção e a carga parasitária, mas não com a condição clínica. Em todos os grupos, IgG total foi o anticorpo predominante, com maior concentração de IgG2 que IgG1. O anticorpo IgG foi positivo em proporção elevada nos animais infectados (SP+ 98,1%; AP+ 95,2%), mas não o IgE (SP+ 80,8%; AP+ 71,2%). O achado mais relevante refere-se aos cães não infectados que apresentaram elevada positividade para anticorpos IgG anti-Leishmania (SP- 60,0%; AP- 44,4%), IgE (SP- 44,0%; AP- 27,8%), IgG1 (SP- 28,0%; AP- 22,2%) e IgG2 (SP- 56,0%; AP- 41,7%). CONCLUSÕES O status sorológico dos cães, determinado por qualquer classe ou subclasse de anticorpos, não distinguiu com acurácia cães infectados por L. (L.) infantum chagasi daqueles não infectados. A imprecisão do resultado sorológico pode prejudicar não só o diagnóstico, mas também as investigações epidemiológicas e as estratégias para o controle da leishmaniose visceral. Esse complexo cenário sorológico observado na área endêmica mostra quão desafiador é o diagnóstico canino, e aponta a dificuldade enfrentada pelos médicos veterinários e coordenadores dos programas de controle.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Faculdade de Medicina. Universidade de São Paulo Departamento de Patologia Laboratório de Patologia de Moléstias Infecciosas/LIM-50Universidade Federal da Bahia Faculdade de Medicina Veterinária e Zootecnia Departamento de Clínica e PatologiaUniversidade Estadual Paulista Faculdade de Medicina Veterinária Departamento de Clínica, Cirurgia e Reprodução AnimalUniversidade Estadual Paulista Faculdade de Medicina Veterinária Departamento de Clínica, Cirurgia e Reprodução AnimalFaculdade de Saúde Pública da Universidade de São PauloUniversidade de São Paulo (USP)Universidade Federal da Bahia (UFBA)Universidade Estadual Paulista (Unesp)Laranjeira, Daniela FariasMatta, Vânia Lúcia Ribeiro DaTomokane, Thaíse YumieMarcondes, Mary [UNESP]Corbet, Carlos Eduardo PereiraLaurenti, Márcia Dalastra2015-02-02T12:39:13Z2015-02-02T12:39:13Z2014-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article563-571application/pdfhttp://dx.doi.org/10.1590/S0034-8910.2014048005224Revista de Saúde Pública. Faculdade de Saúde Pública da Universidade de São Paulo, v. 48, n. 4, p. 563-571, 2014.0034-8910http://hdl.handle.net/11449/11410810.1590/S0034-8910.2014048005224S0034-89102014000400563S0034-89102014000400563.pdf1817946671090010SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Saúde Pública1.9110,807info:eu-repo/semantics/openAccess2023-10-16T06:07:46Zoai:repositorio.unesp.br:11449/114108Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-16T06:07:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area Status sorológico e de infecção canina em área endêmica de leishmaniose visceral |
title |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area |
spellingShingle |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area Laranjeira, Daniela Farias Leishmaniose Visceral, diagnóstico Leishmaniose Visceral, epidemiologia Leishmania, imunologia Cães Estudos Soroepidemiológicos Doenças Endêmicas, veterinária Leishmaniasis Visceral diagnosis epidemiology Leishmania immunology Dogs Seroepidemiologic Studies Endemic Diseases veterinary |
title_short |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area |
title_full |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area |
title_fullStr |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area |
title_full_unstemmed |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area |
title_sort |
Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area |
author |
Laranjeira, Daniela Farias |
author_facet |
Laranjeira, Daniela Farias Matta, Vânia Lúcia Ribeiro Da Tomokane, Thaíse Yumie Marcondes, Mary [UNESP] Corbet, Carlos Eduardo Pereira Laurenti, Márcia Dalastra |
author_role |
author |
author2 |
Matta, Vânia Lúcia Ribeiro Da Tomokane, Thaíse Yumie Marcondes, Mary [UNESP] Corbet, Carlos Eduardo Pereira Laurenti, Márcia Dalastra |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal da Bahia (UFBA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Laranjeira, Daniela Farias Matta, Vânia Lúcia Ribeiro Da Tomokane, Thaíse Yumie Marcondes, Mary [UNESP] Corbet, Carlos Eduardo Pereira Laurenti, Márcia Dalastra |
dc.subject.por.fl_str_mv |
Leishmaniose Visceral, diagnóstico Leishmaniose Visceral, epidemiologia Leishmania, imunologia Cães Estudos Soroepidemiológicos Doenças Endêmicas, veterinária Leishmaniasis Visceral diagnosis epidemiology Leishmania immunology Dogs Seroepidemiologic Studies Endemic Diseases veterinary |
topic |
Leishmaniose Visceral, diagnóstico Leishmaniose Visceral, epidemiologia Leishmania, imunologia Cães Estudos Soroepidemiológicos Doenças Endêmicas, veterinária Leishmaniasis Visceral diagnosis epidemiology Leishmania immunology Dogs Seroepidemiologic Studies Endemic Diseases veterinary |
description |
OBJECTIVEThis study investigated the serological status of dogs living in a visceral leishmaniasis-endemic area and its correlation with the parasitological condition of the animals. METHODSCanine humoral response was evaluated using the sera of 134 dogs by enzyme-linked immunosorbent assay and immunohistochemistry to detect parasites in the skin, lymph node, and spleen of the animals. The specific antibodies investigated were IgG, IgG1, IgG2, and IgE. RESULTSAccording to the parasitological, laboratory, and clinical findings, the dogs were placed into one of four groups: asymptomatic with (AP+, n = 21) or without (AP-, n = 36) Leishmania tissue parasitism and symptomatic with (SP+, n = 52) or without (SP-, n = 25) parasitism. Higher IgG and IgE levels were positively correlated with the infection condition and parasite load, but not with the clinical status. In all groups, total IgG was the predominant antibody, which occurred at the expense of IgG2 instead of IgG1. Most of the infected dogs tested positive for IgG (SP+, 98.1%; AP+, 95.2%), whereas this was not observed with IgE (SP+, 80.8%; AP+, 71.2%). The most relevant finding was the high positivity of the uninfected dogs for Leishmania-specific IgG (SP-, 60.0%; AP-, 44.4%), IgE (SP-, 44.0%; AP-, 27.8%), IgG1 (SP-, 28.0%; AP-, 22.2%), and IgG2 antibodies (SP-, 56.0%; AP-, 41.7%). CONCLUSIONSThe serological status of dogs, as determined by any class or subclass of antibodies, did not accurately distinguish dogs infected with L. (L.) infantum chagasi from uninfected animals. The inaccuracy of the serological result may impair not only the diagnosis, but also epidemiological investigations and strategies for visceral leishmaniasis control. This complex serological scenario occurring in a visceral leishmaniasis-endemic area highlights the challenges associated with canine diagnosis and points out the difficulties experienced by veterinary clinicians and coordinators of control programs. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-01 2015-02-02T12:39:13Z 2015-02-02T12:39:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0034-8910.2014048005224 Revista de Saúde Pública. Faculdade de Saúde Pública da Universidade de São Paulo, v. 48, n. 4, p. 563-571, 2014. 0034-8910 http://hdl.handle.net/11449/114108 10.1590/S0034-8910.2014048005224 S0034-89102014000400563 S0034-89102014000400563.pdf 1817946671090010 |
url |
http://dx.doi.org/10.1590/S0034-8910.2014048005224 http://hdl.handle.net/11449/114108 |
identifier_str_mv |
Revista de Saúde Pública. Faculdade de Saúde Pública da Universidade de São Paulo, v. 48, n. 4, p. 563-571, 2014. 0034-8910 10.1590/S0034-8910.2014048005224 S0034-89102014000400563 S0034-89102014000400563.pdf 1817946671090010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Saúde Pública 1.911 0,807 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
563-571 application/pdf |
dc.publisher.none.fl_str_mv |
Faculdade de Saúde Pública da Universidade de São Paulo |
publisher.none.fl_str_mv |
Faculdade de Saúde Pública da Universidade de São Paulo |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789415677886464 |