Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10340-021-01453-5 http://hdl.handle.net/11449/222810 |
Resumo: | The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a major pest of maize crops and others row crops on the American continent, and this pest has spread to Africa and Asia. Such lepidopteran pest presents resistant populations to synthetic insecticides and to Bacillus thuringiensis (Bt) toxins and, consequently, new (bio) insecticides with distinct modes of action are required. In order to search for derivatives from Solanaceae as a potential source of bioactive compounds, a screening of 38 ethanolic extracts were prepared from 25 Solanaceae species. Using dietary exposure assessment, the ethanolic extracts from leaves of Acnistus arborescens (L.) Schltdl. and Datura stramonium L. were the most promising derivatives. A bioguided fractionation was performed with both plant species. The A. arborescens dichloromethane fraction was the most active, causing sublethal (growth inhibition) and lethal toxicity. Thus, this fraction was submitted to another fractionation procedure resulting in three subfractions (dichloromethane, ethyl acetate and methanol) that were tested against S. frugiperda caterpillars. All subfractions lead to significant sublethal effects, however, without significant mortality. The chemical diversity in the subfractions of A. arborescens was assessed through untargeted approaches, such as molecular networking and the in silico annotation tool NAP, by which two withanolides glycosides were annotated: (22R)-1-Oxo-3beta-(beta-D-glucopyranosyloxy)-14,20,22,27-tetrahydroxyergosta-5,24-diene-26-oic acid delta-lactone and withanoside XI. Therefore, derivatives of Solanaceae present promising compounds that may be useful in the framework of S. frugiperda integrated pest management. In addition, metabolomics is an innovative alternative and powerful approach to facilitate the putative identification of compounds in studies of natural products. |
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Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approachesAcnistus arborescensBotanical insecticidesChromatographic proceduresDatura stramoniumFall armywormWithanolidesThe fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a major pest of maize crops and others row crops on the American continent, and this pest has spread to Africa and Asia. Such lepidopteran pest presents resistant populations to synthetic insecticides and to Bacillus thuringiensis (Bt) toxins and, consequently, new (bio) insecticides with distinct modes of action are required. In order to search for derivatives from Solanaceae as a potential source of bioactive compounds, a screening of 38 ethanolic extracts were prepared from 25 Solanaceae species. Using dietary exposure assessment, the ethanolic extracts from leaves of Acnistus arborescens (L.) Schltdl. and Datura stramonium L. were the most promising derivatives. A bioguided fractionation was performed with both plant species. The A. arborescens dichloromethane fraction was the most active, causing sublethal (growth inhibition) and lethal toxicity. Thus, this fraction was submitted to another fractionation procedure resulting in three subfractions (dichloromethane, ethyl acetate and methanol) that were tested against S. frugiperda caterpillars. All subfractions lead to significant sublethal effects, however, without significant mortality. The chemical diversity in the subfractions of A. arborescens was assessed through untargeted approaches, such as molecular networking and the in silico annotation tool NAP, by which two withanolides glycosides were annotated: (22R)-1-Oxo-3beta-(beta-D-glucopyranosyloxy)-14,20,22,27-tetrahydroxyergosta-5,24-diene-26-oic acid delta-lactone and withanoside XI. Therefore, derivatives of Solanaceae present promising compounds that may be useful in the framework of S. frugiperda integrated pest management. In addition, metabolomics is an innovative alternative and powerful approach to facilitate the putative identification of compounds in studies of natural products.Department of Entomology Lavras Federal University (UFLA)Research Center for Family Agriculture Agricultural Research and Rural Extension Company of Santa Catarina (EPAGRI/CEPAF), Rua Ferdinando Ricieri Tusset S/N, Bairro São Cristóvão, Caixa Postal 791Department of Exact Sciences “Luiz de Queiroz” College of Agriculture (USP/ESALQ) University of São PauloDepartment of Botany São Paulo State University (UNESP)Department of Entomology and Acarology “Luiz de Queiroz” College of Agriculture (USP/ESALQ) University of São PauloDepartment of Botany São Paulo State University (UNESP)Universidade Federal de Lavras (UFLA)Agricultural Research and Rural Extension Company of Santa Catarina (EPAGRI/CEPAF)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Lima, Andreísa Fabrido Prado Ribeiro, LeandroGonçalves, Gabriel Luiz PadoanMaimone, Naydja MorallesGissi, Danilo Soares [UNESP]de Lira, Simone PossedenteVendramim, José Djair2022-04-28T19:46:57Z2022-04-28T19:46:57Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10340-021-01453-5Journal of Pest Science.1612-47661612-4758http://hdl.handle.net/11449/22281010.1007/s10340-021-01453-52-s2.0-85118595966Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Pest Scienceinfo:eu-repo/semantics/openAccess2022-04-28T19:46:57Zoai:repositorio.unesp.br:11449/222810Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:46:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
title |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
spellingShingle |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches Lima, Andreísa Fabri Acnistus arborescens Botanical insecticides Chromatographic procedures Datura stramonium Fall armyworm Withanolides |
title_short |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
title_full |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
title_fullStr |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
title_full_unstemmed |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
title_sort |
Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches |
author |
Lima, Andreísa Fabri |
author_facet |
Lima, Andreísa Fabri do Prado Ribeiro, Leandro Gonçalves, Gabriel Luiz Padoan Maimone, Naydja Moralles Gissi, Danilo Soares [UNESP] de Lira, Simone Possedente Vendramim, José Djair |
author_role |
author |
author2 |
do Prado Ribeiro, Leandro Gonçalves, Gabriel Luiz Padoan Maimone, Naydja Moralles Gissi, Danilo Soares [UNESP] de Lira, Simone Possedente Vendramim, José Djair |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Lavras (UFLA) Agricultural Research and Rural Extension Company of Santa Catarina (EPAGRI/CEPAF) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Lima, Andreísa Fabri do Prado Ribeiro, Leandro Gonçalves, Gabriel Luiz Padoan Maimone, Naydja Moralles Gissi, Danilo Soares [UNESP] de Lira, Simone Possedente Vendramim, José Djair |
dc.subject.por.fl_str_mv |
Acnistus arborescens Botanical insecticides Chromatographic procedures Datura stramonium Fall armyworm Withanolides |
topic |
Acnistus arborescens Botanical insecticides Chromatographic procedures Datura stramonium Fall armyworm Withanolides |
description |
The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a major pest of maize crops and others row crops on the American continent, and this pest has spread to Africa and Asia. Such lepidopteran pest presents resistant populations to synthetic insecticides and to Bacillus thuringiensis (Bt) toxins and, consequently, new (bio) insecticides with distinct modes of action are required. In order to search for derivatives from Solanaceae as a potential source of bioactive compounds, a screening of 38 ethanolic extracts were prepared from 25 Solanaceae species. Using dietary exposure assessment, the ethanolic extracts from leaves of Acnistus arborescens (L.) Schltdl. and Datura stramonium L. were the most promising derivatives. A bioguided fractionation was performed with both plant species. The A. arborescens dichloromethane fraction was the most active, causing sublethal (growth inhibition) and lethal toxicity. Thus, this fraction was submitted to another fractionation procedure resulting in three subfractions (dichloromethane, ethyl acetate and methanol) that were tested against S. frugiperda caterpillars. All subfractions lead to significant sublethal effects, however, without significant mortality. The chemical diversity in the subfractions of A. arborescens was assessed through untargeted approaches, such as molecular networking and the in silico annotation tool NAP, by which two withanolides glycosides were annotated: (22R)-1-Oxo-3beta-(beta-D-glucopyranosyloxy)-14,20,22,27-tetrahydroxyergosta-5,24-diene-26-oic acid delta-lactone and withanoside XI. Therefore, derivatives of Solanaceae present promising compounds that may be useful in the framework of S. frugiperda integrated pest management. In addition, metabolomics is an innovative alternative and powerful approach to facilitate the putative identification of compounds in studies of natural products. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:46:57Z 2022-04-28T19:46:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10340-021-01453-5 Journal of Pest Science. 1612-4766 1612-4758 http://hdl.handle.net/11449/222810 10.1007/s10340-021-01453-5 2-s2.0-85118595966 |
url |
http://dx.doi.org/10.1007/s10340-021-01453-5 http://hdl.handle.net/11449/222810 |
identifier_str_mv |
Journal of Pest Science. 1612-4766 1612-4758 10.1007/s10340-021-01453-5 2-s2.0-85118595966 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Pest Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965475266887681 |