Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-017-2085-3 http://hdl.handle.net/11449/174302 |
Resumo: | Objective: The study aims to evaluate the odontogenic potential of human dental pulp cells (HDPCs) in contact with an experimental porous chitosan-collagen scaffold (CHC) enriched or not with a mineral phase of calcium-aluminate (CHC-CA). Material and methods: To assess the chemotactic effect of the materials, we placed HDPCs seeded on transwell membranes in intimate contact with the CHC or CHC-CA surface, and the cell migration was monitored for 48 h. Additionally, cells were seeded onto the material surface, and the viability and proliferation were evaluated at several time points. To assess the odontoblastic differentiation, we evaluated ALP activity, DSPP/DMP-1 gene expression, and mineralized matrix deposition. HDPCs cultured onto a polystyrene surface (monolayer) were used as negative control group. Results: The experimental CHC-CA scaffold induced intense migration of HDPCs through transwell membranes, with cells attaching to and spreading on the material surface after 24-h incubation. Also, the HDPCs seeded onto the CHC-CA scaffold were capable of migrating inside it, remaining viable and featuring a proliferative rate more rapid than that of CHC and control groups at 7 and 14 days of cell culture. At long-term culture, cells in the CHC-CA scaffold featured the highest deposition of mineralized matrix and expression of odontoblastic markers (ALP activity and DSPP/DMP-1 gene expression). Conclusions: According to the results, the CHC-CA scaffold is a bioactive and cytocompatible material capable of increasing the odontogenic potential of human pulp cells. Based on analysis of the positive data obtained in this study, one can suggest that the CHC-CA scaffold is an interesting future candidate for the treatment of exposed pulps. Clinical relevance: The experimental scaffold composed by a chitosan-collagen matrix mineralized with calcium aluminate seems to be an interesting candidate for in vivo application as a cell-free approach to dentin tissue engineering, which may open a new perspective for the treatment of exposed pulp tissue. |
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Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffoldEndodonticsExtracellular matrixRegenerative medicineStem cellsTissue engineeringObjective: The study aims to evaluate the odontogenic potential of human dental pulp cells (HDPCs) in contact with an experimental porous chitosan-collagen scaffold (CHC) enriched or not with a mineral phase of calcium-aluminate (CHC-CA). Material and methods: To assess the chemotactic effect of the materials, we placed HDPCs seeded on transwell membranes in intimate contact with the CHC or CHC-CA surface, and the cell migration was monitored for 48 h. Additionally, cells were seeded onto the material surface, and the viability and proliferation were evaluated at several time points. To assess the odontoblastic differentiation, we evaluated ALP activity, DSPP/DMP-1 gene expression, and mineralized matrix deposition. HDPCs cultured onto a polystyrene surface (monolayer) were used as negative control group. Results: The experimental CHC-CA scaffold induced intense migration of HDPCs through transwell membranes, with cells attaching to and spreading on the material surface after 24-h incubation. Also, the HDPCs seeded onto the CHC-CA scaffold were capable of migrating inside it, remaining viable and featuring a proliferative rate more rapid than that of CHC and control groups at 7 and 14 days of cell culture. At long-term culture, cells in the CHC-CA scaffold featured the highest deposition of mineralized matrix and expression of odontoblastic markers (ALP activity and DSPP/DMP-1 gene expression). Conclusions: According to the results, the CHC-CA scaffold is a bioactive and cytocompatible material capable of increasing the odontogenic potential of human pulp cells. Based on analysis of the positive data obtained in this study, one can suggest that the CHC-CA scaffold is an interesting future candidate for the treatment of exposed pulps. Clinical relevance: The experimental scaffold composed by a chitosan-collagen matrix mineralized with calcium aluminate seems to be an interesting candidate for in vivo application as a cell-free approach to dentin tissue engineering, which may open a new perspective for the treatment of exposed pulp tissue.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista – UNESP, Humaitá Street, 1680Ribeirão Preto School of Medicine São Paulo University – USP, Avenida do CaféDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista – UNESP, Humaitá Street, 1680Department of Operative Dentistry Araraquara School of Dentistry University Estadual Paulista – UNESP, Humaitá Street, 1680Department of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista – UNESP, Humaitá Street, 1680Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista – UNESP, Humaitá Street, 1680Department of Operative Dentistry Araraquara School of Dentistry University Estadual Paulista – UNESP, Humaitá Street, 1680FAPESP: 2013/23520-0FAPESP: 2014/13034-3CNPq: 303599/2014-6CNPq: 442336/2014-4Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Soares, Diana Gabriela [UNESP]Rosseto, Hebert LuísScheffel, Débora Salles [UNESP]Basso, Fernanda Gonçalves [UNESP]Huck, Claudia [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos Alberto [UNESP]2018-12-11T17:10:20Z2018-12-11T17:10:20Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2827-2839application/pdfhttp://dx.doi.org/10.1007/s00784-017-2085-3Clinical Oral Investigations, v. 21, n. 9, p. 2827-2839, 2017.1436-37711432-6981http://hdl.handle.net/11449/17430210.1007/s00784-017-2085-32-s2.0-850146889812-s2.0-85014688981.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2023-10-21T06:09:10Zoai:repositorio.unesp.br:11449/174302Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-21T06:09:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
title |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
spellingShingle |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold Soares, Diana Gabriela [UNESP] Endodontics Extracellular matrix Regenerative medicine Stem cells Tissue engineering |
title_short |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
title_full |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
title_fullStr |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
title_full_unstemmed |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
title_sort |
Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold |
author |
Soares, Diana Gabriela [UNESP] |
author_facet |
Soares, Diana Gabriela [UNESP] Rosseto, Hebert Luís Scheffel, Débora Salles [UNESP] Basso, Fernanda Gonçalves [UNESP] Huck, Claudia [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Rosseto, Hebert Luís Scheffel, Débora Salles [UNESP] Basso, Fernanda Gonçalves [UNESP] Huck, Claudia [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Soares, Diana Gabriela [UNESP] Rosseto, Hebert Luís Scheffel, Débora Salles [UNESP] Basso, Fernanda Gonçalves [UNESP] Huck, Claudia [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Endodontics Extracellular matrix Regenerative medicine Stem cells Tissue engineering |
topic |
Endodontics Extracellular matrix Regenerative medicine Stem cells Tissue engineering |
description |
Objective: The study aims to evaluate the odontogenic potential of human dental pulp cells (HDPCs) in contact with an experimental porous chitosan-collagen scaffold (CHC) enriched or not with a mineral phase of calcium-aluminate (CHC-CA). Material and methods: To assess the chemotactic effect of the materials, we placed HDPCs seeded on transwell membranes in intimate contact with the CHC or CHC-CA surface, and the cell migration was monitored for 48 h. Additionally, cells were seeded onto the material surface, and the viability and proliferation were evaluated at several time points. To assess the odontoblastic differentiation, we evaluated ALP activity, DSPP/DMP-1 gene expression, and mineralized matrix deposition. HDPCs cultured onto a polystyrene surface (monolayer) were used as negative control group. Results: The experimental CHC-CA scaffold induced intense migration of HDPCs through transwell membranes, with cells attaching to and spreading on the material surface after 24-h incubation. Also, the HDPCs seeded onto the CHC-CA scaffold were capable of migrating inside it, remaining viable and featuring a proliferative rate more rapid than that of CHC and control groups at 7 and 14 days of cell culture. At long-term culture, cells in the CHC-CA scaffold featured the highest deposition of mineralized matrix and expression of odontoblastic markers (ALP activity and DSPP/DMP-1 gene expression). Conclusions: According to the results, the CHC-CA scaffold is a bioactive and cytocompatible material capable of increasing the odontogenic potential of human pulp cells. Based on analysis of the positive data obtained in this study, one can suggest that the CHC-CA scaffold is an interesting future candidate for the treatment of exposed pulps. Clinical relevance: The experimental scaffold composed by a chitosan-collagen matrix mineralized with calcium aluminate seems to be an interesting candidate for in vivo application as a cell-free approach to dentin tissue engineering, which may open a new perspective for the treatment of exposed pulp tissue. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-12-11T17:10:20Z 2018-12-11T17:10:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-017-2085-3 Clinical Oral Investigations, v. 21, n. 9, p. 2827-2839, 2017. 1436-3771 1432-6981 http://hdl.handle.net/11449/174302 10.1007/s00784-017-2085-3 2-s2.0-85014688981 2-s2.0-85014688981.pdf |
url |
http://dx.doi.org/10.1007/s00784-017-2085-3 http://hdl.handle.net/11449/174302 |
identifier_str_mv |
Clinical Oral Investigations, v. 21, n. 9, p. 2827-2839, 2017. 1436-3771 1432-6981 10.1007/s00784-017-2085-3 2-s2.0-85014688981 2-s2.0-85014688981.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2827-2839 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964646101221376 |