Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii

Detalhes bibliográficos
Autor(a) principal: Marcolan De Mello, Mirian [UNESP]
Data de Publicação: 2019
Outros Autores: De Barros, Patrícia Pimentel [UNESP], de Cassia Bernardes, Renata, Alves, Silvio Rubens, Ramanzini, Naiara Pires [UNESP], Figueiredo-Godoi, Lívia Mara Alves [UNESP], Prado, Ana Carolina Chipoletti [UNESP], Jorge, Antonio Olavo Cardoso [UNESP], Junqueira, Juliana Campos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-019-02773-w
http://hdl.handle.net/11449/190207
Resumo: Infections caused by Acinetobacter baumannii have become a challenge for healthcare professionals because of the rapid increase in Gram-negative bacteria resistant to carbapenem antibiotics. The objective of this study was to evaluate the effect of antimicrobial photodynamic therapy (aPDT) against different strains of A. baumannii isolated from patients with infectious process and hospitalized at the intensive care unit of the hospitals of São Jose dos Campos, São Paulo. These isolates were obtained from the Valeclin Clinical Analysis Laboratory (SP, Brazil) and were tested for susceptibility to the carbapenems imipenem and meropenem by determination of the minimal inhibitory concentration (MIC) using the broth microdilution method. The strains susceptible and resistant to these antibiotics were submitted to aPDT using methylene blue and a low-level laser with a wavelength of 660 nm and fluence of 39.5 J/cm 2 (energy of 15 J and time of 428 s). The number of colony-forming units (CFU/mL) was analyzed by ANOVA and the Tukey test. The laboratory of origin of the clinical isolates identified 1.54% of 13,715 strains tested over a period of 8 months as A. baumannii. Among the A. baumannii isolates, 58% were resistant to carbapenems by the disk diffusion test. Susceptible isolates exhibited MIC of 0.5 to 1 μg/mL and resistant isolates of 64 to > 128 μg/mL. PDT reduced the number of A. baumannii cells for all isolates tested, with this reduction ranging from 63 to 88% for susceptible isolates and from 26 to 97% for resistant isolates. The percentage of viability was dependent on the strain analyzed. In conclusion, these data indicate that PDT could be an alternative strategy for the control of infections caused by carbapenem-resistant A. baumannii.
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spelling Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumanniiAcinetobacter baumanniiAntimicrobial photodynamic therapyAntimicrobial resistanceAntimicrobialsInfections caused by Acinetobacter baumannii have become a challenge for healthcare professionals because of the rapid increase in Gram-negative bacteria resistant to carbapenem antibiotics. The objective of this study was to evaluate the effect of antimicrobial photodynamic therapy (aPDT) against different strains of A. baumannii isolated from patients with infectious process and hospitalized at the intensive care unit of the hospitals of São Jose dos Campos, São Paulo. These isolates were obtained from the Valeclin Clinical Analysis Laboratory (SP, Brazil) and were tested for susceptibility to the carbapenems imipenem and meropenem by determination of the minimal inhibitory concentration (MIC) using the broth microdilution method. The strains susceptible and resistant to these antibiotics were submitted to aPDT using methylene blue and a low-level laser with a wavelength of 660 nm and fluence of 39.5 J/cm 2 (energy of 15 J and time of 428 s). The number of colony-forming units (CFU/mL) was analyzed by ANOVA and the Tukey test. The laboratory of origin of the clinical isolates identified 1.54% of 13,715 strains tested over a period of 8 months as A. baumannii. Among the A. baumannii isolates, 58% were resistant to carbapenems by the disk diffusion test. Susceptible isolates exhibited MIC of 0.5 to 1 μg/mL and resistant isolates of 64 to > 128 μg/mL. PDT reduced the number of A. baumannii cells for all isolates tested, with this reduction ranging from 63 to 88% for susceptible isolates and from 26 to 97% for resistant isolates. The percentage of viability was dependent on the strain analyzed. In conclusion, these data indicate that PDT could be an alternative strategy for the control of infections caused by carbapenem-resistant A. baumannii.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo 777, São DimasValeclin Clinical Analysis LaboratoryDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo 777, São DimasUniversidade Estadual Paulista (Unesp)Valeclin Clinical Analysis LaboratoryMarcolan De Mello, Mirian [UNESP]De Barros, Patrícia Pimentel [UNESP]de Cassia Bernardes, RenataAlves, Silvio RubensRamanzini, Naiara Pires [UNESP]Figueiredo-Godoi, Lívia Mara Alves [UNESP]Prado, Ana Carolina Chipoletti [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos [UNESP]2019-10-06T17:05:46Z2019-10-06T17:05:46Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10103-019-02773-wLasers in Medical Science.1435-604X0268-8921http://hdl.handle.net/11449/19020710.1007/s10103-019-02773-w2-s2.0-85063188788Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2021-10-23T02:05:44Zoai:repositorio.unesp.br:11449/190207Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T02:05:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
title Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
spellingShingle Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
Marcolan De Mello, Mirian [UNESP]
Acinetobacter baumannii
Antimicrobial photodynamic therapy
Antimicrobial resistance
Antimicrobials
title_short Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
title_full Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
title_fullStr Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
title_full_unstemmed Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
title_sort Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii
author Marcolan De Mello, Mirian [UNESP]
author_facet Marcolan De Mello, Mirian [UNESP]
De Barros, Patrícia Pimentel [UNESP]
de Cassia Bernardes, Renata
Alves, Silvio Rubens
Ramanzini, Naiara Pires [UNESP]
Figueiredo-Godoi, Lívia Mara Alves [UNESP]
Prado, Ana Carolina Chipoletti [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
author_role author
author2 De Barros, Patrícia Pimentel [UNESP]
de Cassia Bernardes, Renata
Alves, Silvio Rubens
Ramanzini, Naiara Pires [UNESP]
Figueiredo-Godoi, Lívia Mara Alves [UNESP]
Prado, Ana Carolina Chipoletti [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Valeclin Clinical Analysis Laboratory
dc.contributor.author.fl_str_mv Marcolan De Mello, Mirian [UNESP]
De Barros, Patrícia Pimentel [UNESP]
de Cassia Bernardes, Renata
Alves, Silvio Rubens
Ramanzini, Naiara Pires [UNESP]
Figueiredo-Godoi, Lívia Mara Alves [UNESP]
Prado, Ana Carolina Chipoletti [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
dc.subject.por.fl_str_mv Acinetobacter baumannii
Antimicrobial photodynamic therapy
Antimicrobial resistance
Antimicrobials
topic Acinetobacter baumannii
Antimicrobial photodynamic therapy
Antimicrobial resistance
Antimicrobials
description Infections caused by Acinetobacter baumannii have become a challenge for healthcare professionals because of the rapid increase in Gram-negative bacteria resistant to carbapenem antibiotics. The objective of this study was to evaluate the effect of antimicrobial photodynamic therapy (aPDT) against different strains of A. baumannii isolated from patients with infectious process and hospitalized at the intensive care unit of the hospitals of São Jose dos Campos, São Paulo. These isolates were obtained from the Valeclin Clinical Analysis Laboratory (SP, Brazil) and were tested for susceptibility to the carbapenems imipenem and meropenem by determination of the minimal inhibitory concentration (MIC) using the broth microdilution method. The strains susceptible and resistant to these antibiotics were submitted to aPDT using methylene blue and a low-level laser with a wavelength of 660 nm and fluence of 39.5 J/cm 2 (energy of 15 J and time of 428 s). The number of colony-forming units (CFU/mL) was analyzed by ANOVA and the Tukey test. The laboratory of origin of the clinical isolates identified 1.54% of 13,715 strains tested over a period of 8 months as A. baumannii. Among the A. baumannii isolates, 58% were resistant to carbapenems by the disk diffusion test. Susceptible isolates exhibited MIC of 0.5 to 1 μg/mL and resistant isolates of 64 to > 128 μg/mL. PDT reduced the number of A. baumannii cells for all isolates tested, with this reduction ranging from 63 to 88% for susceptible isolates and from 26 to 97% for resistant isolates. The percentage of viability was dependent on the strain analyzed. In conclusion, these data indicate that PDT could be an alternative strategy for the control of infections caused by carbapenem-resistant A. baumannii.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T17:05:46Z
2019-10-06T17:05:46Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-019-02773-w
Lasers in Medical Science.
1435-604X
0268-8921
http://hdl.handle.net/11449/190207
10.1007/s10103-019-02773-w
2-s2.0-85063188788
url http://dx.doi.org/10.1007/s10103-019-02773-w
http://hdl.handle.net/11449/190207
identifier_str_mv Lasers in Medical Science.
1435-604X
0268-8921
10.1007/s10103-019-02773-w
2-s2.0-85063188788
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Medical Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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