Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4317/medoral.23854 http://hdl.handle.net/11449/209082 |
Resumo: | Background: Whole Slide Imaging (WSI) is an alternative method to light microscopy (LM). However, few studies have compared the diagnostic agreement between WSI and LM, especially to grade oral epithelial dysplasia (OED). The purpose of this study was to evaluate the variability in grading OED by the World Health Organization grading system, using WSI and conventional LM, and to investigate whether the access to clinical information, and psychologic or physical states of the pathologists could interfere with the diagnosis. Material and Methods: eleven experienced pathologists from seven Brazilian universities independently evaluated twenty-five OED cases. The analyses were performed in duplicate for each method, with an interval of at least 30 days, and the time consumed in each analysis was measured. Physical and psychologic states were evaluated by blood pressure levels, heart rate and two questionnaires: State-Trait Anxiety Inventory and Perceived Stress Scale. Clinical information was provided after the second evaluation using WSI and the pathologist could change their diagnostic decision or not. Results: LM showed a higher inter-examiner agreement (k=0.53) than WSI (k=0.45) and a smaller time consumed by the pathologists (mean of 65.53 seconds compared to 91.02 seconds in WSI). In the first analysis using conventional microscopy, there was a positive correlation between kappa values and anxiety (r=0.47, p=0.02), and stress (r=0.64, p<0.01), and an inverse correlation with heart rate (r=-0.48, p=0.02). In the digital analysis, there was also a positive correlation between kappa values and anxiety (r=0.75, p<0.001). After clinical information was given, there was a slight change in 11.3% of the cases, and a great discrepancy in 1.1% of the cases, mainly increasing the OED grade. Conclusions: both microscopy systems had similar results, although LM had slightly higher kappa values, and WSI was more time consuming. |
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Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional studyPathologymicroscopydiagnosisleukoplakia oralanxietyBackground: Whole Slide Imaging (WSI) is an alternative method to light microscopy (LM). However, few studies have compared the diagnostic agreement between WSI and LM, especially to grade oral epithelial dysplasia (OED). The purpose of this study was to evaluate the variability in grading OED by the World Health Organization grading system, using WSI and conventional LM, and to investigate whether the access to clinical information, and psychologic or physical states of the pathologists could interfere with the diagnosis. Material and Methods: eleven experienced pathologists from seven Brazilian universities independently evaluated twenty-five OED cases. The analyses were performed in duplicate for each method, with an interval of at least 30 days, and the time consumed in each analysis was measured. Physical and psychologic states were evaluated by blood pressure levels, heart rate and two questionnaires: State-Trait Anxiety Inventory and Perceived Stress Scale. Clinical information was provided after the second evaluation using WSI and the pathologist could change their diagnostic decision or not. Results: LM showed a higher inter-examiner agreement (k=0.53) than WSI (k=0.45) and a smaller time consumed by the pathologists (mean of 65.53 seconds compared to 91.02 seconds in WSI). In the first analysis using conventional microscopy, there was a positive correlation between kappa values and anxiety (r=0.47, p=0.02), and stress (r=0.64, p<0.01), and an inverse correlation with heart rate (r=-0.48, p=0.02). In the digital analysis, there was also a positive correlation between kappa values and anxiety (r=0.75, p<0.001). After clinical information was given, there was a slight change in 11.3% of the cases, and a great discrepancy in 1.1% of the cases, mainly increasing the OED grade. Conclusions: both microscopy systems had similar results, although LM had slightly higher kappa values, and WSI was more time consuming.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Campinas Unicamp, Piracicaba Dent Sch, Dept Oral Diag, 901 Limeira Av, Piracicaba, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu, SP, BrazilCAPES: 001FAPESP: 2018/24715-2Medicina Oral S LUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Diniz, Priscilla BarbosaSena-Filho, MarcondesGraner, Karen Mendes [UNESP]Linhares Almeida Mariz, Bruno AugustoReis, Leonardo Amaral dosAlmeida, Oslei Paes deJorge, Jacks2021-06-25T11:48:03Z2021-06-25T11:48:03Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleE8-E13http://dx.doi.org/10.4317/medoral.23854Medicina Oral Patologia Oral Y Cirugia Bucal. Valencia: Medicina Oral S L, v. 26, n. 1, p. E8-E13, 2021.1698-6946http://hdl.handle.net/11449/20908210.4317/medoral.23854WOS:000604964100016Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedicina Oral Patologia Oral Y Cirugia Bucalinfo:eu-repo/semantics/openAccess2021-10-23T19:23:33Zoai:repositorio.unesp.br:11449/209082Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
title |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
spellingShingle |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study Diniz, Priscilla Barbosa Pathology microscopy diagnosis leukoplakia oral anxiety |
title_short |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
title_full |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
title_fullStr |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
title_full_unstemmed |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
title_sort |
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study |
author |
Diniz, Priscilla Barbosa |
author_facet |
Diniz, Priscilla Barbosa Sena-Filho, Marcondes Graner, Karen Mendes [UNESP] Linhares Almeida Mariz, Bruno Augusto Reis, Leonardo Amaral dos Almeida, Oslei Paes de Jorge, Jacks |
author_role |
author |
author2 |
Sena-Filho, Marcondes Graner, Karen Mendes [UNESP] Linhares Almeida Mariz, Bruno Augusto Reis, Leonardo Amaral dos Almeida, Oslei Paes de Jorge, Jacks |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Diniz, Priscilla Barbosa Sena-Filho, Marcondes Graner, Karen Mendes [UNESP] Linhares Almeida Mariz, Bruno Augusto Reis, Leonardo Amaral dos Almeida, Oslei Paes de Jorge, Jacks |
dc.subject.por.fl_str_mv |
Pathology microscopy diagnosis leukoplakia oral anxiety |
topic |
Pathology microscopy diagnosis leukoplakia oral anxiety |
description |
Background: Whole Slide Imaging (WSI) is an alternative method to light microscopy (LM). However, few studies have compared the diagnostic agreement between WSI and LM, especially to grade oral epithelial dysplasia (OED). The purpose of this study was to evaluate the variability in grading OED by the World Health Organization grading system, using WSI and conventional LM, and to investigate whether the access to clinical information, and psychologic or physical states of the pathologists could interfere with the diagnosis. Material and Methods: eleven experienced pathologists from seven Brazilian universities independently evaluated twenty-five OED cases. The analyses were performed in duplicate for each method, with an interval of at least 30 days, and the time consumed in each analysis was measured. Physical and psychologic states were evaluated by blood pressure levels, heart rate and two questionnaires: State-Trait Anxiety Inventory and Perceived Stress Scale. Clinical information was provided after the second evaluation using WSI and the pathologist could change their diagnostic decision or not. Results: LM showed a higher inter-examiner agreement (k=0.53) than WSI (k=0.45) and a smaller time consumed by the pathologists (mean of 65.53 seconds compared to 91.02 seconds in WSI). In the first analysis using conventional microscopy, there was a positive correlation between kappa values and anxiety (r=0.47, p=0.02), and stress (r=0.64, p<0.01), and an inverse correlation with heart rate (r=-0.48, p=0.02). In the digital analysis, there was also a positive correlation between kappa values and anxiety (r=0.75, p<0.001). After clinical information was given, there was a slight change in 11.3% of the cases, and a great discrepancy in 1.1% of the cases, mainly increasing the OED grade. Conclusions: both microscopy systems had similar results, although LM had slightly higher kappa values, and WSI was more time consuming. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:48:03Z 2021-06-25T11:48:03Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4317/medoral.23854 Medicina Oral Patologia Oral Y Cirugia Bucal. Valencia: Medicina Oral S L, v. 26, n. 1, p. E8-E13, 2021. 1698-6946 http://hdl.handle.net/11449/209082 10.4317/medoral.23854 WOS:000604964100016 |
url |
http://dx.doi.org/10.4317/medoral.23854 http://hdl.handle.net/11449/209082 |
identifier_str_mv |
Medicina Oral Patologia Oral Y Cirugia Bucal. Valencia: Medicina Oral S L, v. 26, n. 1, p. E8-E13, 2021. 1698-6946 10.4317/medoral.23854 WOS:000604964100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medicina Oral Patologia Oral Y Cirugia Bucal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
E8-E13 |
dc.publisher.none.fl_str_mv |
Medicina Oral S L |
publisher.none.fl_str_mv |
Medicina Oral S L |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789410317565952 |