Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1677-5538.IBJU.2016.0168 http://hdl.handle.net/11449/159272 |
Resumo: | Purpose: To develop a rabbit experimental study to test the hypothesis that surgical repair of hypospadias with severe ventral curvatures might be completed in one stage, if a graft, such as buccal mucosa, could be placed over the tunica vaginalis flap used in corporoplasty for ventral lengthening, with the addition of an onlay preputial island flap to complete the urethroplasty. Materials and methods: The experimental procedure with rabbits included a tunica vaginalis flap for reconstruction of the corpora after corporotomy, simulating a ventral lengthening operation. A buccal mucosa graft was placed directly on top of the flap, and the urethroplasty was completed with an onlay preputial island flap. Eight rabbits were divided into 4 groups, sacrificed at 2, 4, 8 and 12 weeks postoperatively, and submitted to histological evaluation. Results: We observed a large number of complications, such as fistula (75%), urinary retention (50%) and stenosis (50%). There were two deaths related to the procedure. Histological evaluation demonstrated a severe and persistent inflammatory reaction. No viable tunica vaginalis or buccal mucosa was identified. Conclusions: In this animal model, the association of a buccal mucosa graft over the tunica vaginalis flap was not successful, and resulted in complete loss of both tissues. |
id |
UNSP_23e421c2a6bd341dde5407d4881a239c |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/159272 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbitsHypospadiasUrogenital AbnormalitiesUrologic Surgical ProceduresPurpose: To develop a rabbit experimental study to test the hypothesis that surgical repair of hypospadias with severe ventral curvatures might be completed in one stage, if a graft, such as buccal mucosa, could be placed over the tunica vaginalis flap used in corporoplasty for ventral lengthening, with the addition of an onlay preputial island flap to complete the urethroplasty. Materials and methods: The experimental procedure with rabbits included a tunica vaginalis flap for reconstruction of the corpora after corporotomy, simulating a ventral lengthening operation. A buccal mucosa graft was placed directly on top of the flap, and the urethroplasty was completed with an onlay preputial island flap. Eight rabbits were divided into 4 groups, sacrificed at 2, 4, 8 and 12 weeks postoperatively, and submitted to histological evaluation. Results: We observed a large number of complications, such as fistula (75%), urinary retention (50%) and stenosis (50%). There were two deaths related to the procedure. Histological evaluation demonstrated a severe and persistent inflammatory reaction. No viable tunica vaginalis or buccal mucosa was identified. Conclusions: In this animal model, the association of a buccal mucosa graft over the tunica vaginalis flap was not successful, and resulted in complete loss of both tissues.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Paulo, Sao Paulo, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilBrazilian Soc UrolUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Mattos, Ricardo Marcondes deAraujo, Sergio R. R. de [UNESP]Quitzan, Juliany Gomes [UNESP]Leslie, BrunoBacelar, HerickGomes Parizi, Joao LuizCaetano Martins, Gustavo MarconiCruz, Marcela Leal daMacedo, Antonio2018-11-26T15:37:44Z2018-11-26T15:37:44Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1228-1236application/pdfhttp://dx.doi.org/10.1590/S1677-5538.IBJU.2016.0168International Braz J Urol. Rio De Janeiro: Brazilian Soc Urol, v. 42, n. 6, p. 1228-1236, 2016.1677-5538http://hdl.handle.net/11449/15927210.1590/S1677-5538.IBJU.2016.0168S1677-55382016000601228WOS:000391025600024S1677-55382016000601228.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Braz J Urol0,367info:eu-repo/semantics/openAccess2023-11-12T06:10:09Zoai:repositorio.unesp.br:11449/159272Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-12T06:10:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
title |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
spellingShingle |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits Mattos, Ricardo Marcondes de Hypospadias Urogenital Abnormalities Urologic Surgical Procedures |
title_short |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
title_full |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
title_fullStr |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
title_full_unstemmed |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
title_sort |
Can a graft be placed over a flap in complex hypospadias surgery? An experimental study in rabbits |
author |
Mattos, Ricardo Marcondes de |
author_facet |
Mattos, Ricardo Marcondes de Araujo, Sergio R. R. de [UNESP] Quitzan, Juliany Gomes [UNESP] Leslie, Bruno Bacelar, Herick Gomes Parizi, Joao Luiz Caetano Martins, Gustavo Marconi Cruz, Marcela Leal da Macedo, Antonio |
author_role |
author |
author2 |
Araujo, Sergio R. R. de [UNESP] Quitzan, Juliany Gomes [UNESP] Leslie, Bruno Bacelar, Herick Gomes Parizi, Joao Luiz Caetano Martins, Gustavo Marconi Cruz, Marcela Leal da Macedo, Antonio |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mattos, Ricardo Marcondes de Araujo, Sergio R. R. de [UNESP] Quitzan, Juliany Gomes [UNESP] Leslie, Bruno Bacelar, Herick Gomes Parizi, Joao Luiz Caetano Martins, Gustavo Marconi Cruz, Marcela Leal da Macedo, Antonio |
dc.subject.por.fl_str_mv |
Hypospadias Urogenital Abnormalities Urologic Surgical Procedures |
topic |
Hypospadias Urogenital Abnormalities Urologic Surgical Procedures |
description |
Purpose: To develop a rabbit experimental study to test the hypothesis that surgical repair of hypospadias with severe ventral curvatures might be completed in one stage, if a graft, such as buccal mucosa, could be placed over the tunica vaginalis flap used in corporoplasty for ventral lengthening, with the addition of an onlay preputial island flap to complete the urethroplasty. Materials and methods: The experimental procedure with rabbits included a tunica vaginalis flap for reconstruction of the corpora after corporotomy, simulating a ventral lengthening operation. A buccal mucosa graft was placed directly on top of the flap, and the urethroplasty was completed with an onlay preputial island flap. Eight rabbits were divided into 4 groups, sacrificed at 2, 4, 8 and 12 weeks postoperatively, and submitted to histological evaluation. Results: We observed a large number of complications, such as fistula (75%), urinary retention (50%) and stenosis (50%). There were two deaths related to the procedure. Histological evaluation demonstrated a severe and persistent inflammatory reaction. No viable tunica vaginalis or buccal mucosa was identified. Conclusions: In this animal model, the association of a buccal mucosa graft over the tunica vaginalis flap was not successful, and resulted in complete loss of both tissues. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T15:37:44Z 2018-11-26T15:37:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1677-5538.IBJU.2016.0168 International Braz J Urol. Rio De Janeiro: Brazilian Soc Urol, v. 42, n. 6, p. 1228-1236, 2016. 1677-5538 http://hdl.handle.net/11449/159272 10.1590/S1677-5538.IBJU.2016.0168 S1677-55382016000601228 WOS:000391025600024 S1677-55382016000601228.pdf |
url |
http://dx.doi.org/10.1590/S1677-5538.IBJU.2016.0168 http://hdl.handle.net/11449/159272 |
identifier_str_mv |
International Braz J Urol. Rio De Janeiro: Brazilian Soc Urol, v. 42, n. 6, p. 1228-1236, 2016. 1677-5538 10.1590/S1677-5538.IBJU.2016.0168 S1677-55382016000601228 WOS:000391025600024 S1677-55382016000601228.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Braz J Urol 0,367 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1228-1236 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Soc Urol |
publisher.none.fl_str_mv |
Brazilian Soc Urol |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964905593372672 |