Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201302289 http://hdl.handle.net/11449/126185 |
Resumo: | Methicillin-resistant Staphylococcus aureus (MRSA) can grow as structured biofilm in different surfaces, including oral mucosa and denture surfaces. Such biofilms can be released into the oral fluids and aspirated, causing systemic infections such as aspiration pneumonia. This study evaluated the efficacy of two disinfectant solutions and microwave irradiation in disinfecting acrylic specimens contaminated with MRSA biofilm. Thirty-six acrylic specimens were made, sterilized and contaminated with MRSA (107 cfu/mL). After incubation (37 °C/48 h), the specimens were divided into 4 groups: not disinfected (positive control); soaking in 1% sodium hypochlorite for 10 min; soaking in 2% chlorhexidine gluconate for 10 min; and irradiating by microwave for 3 min at 650 W. The viability of cells was evaluated by XTT reduction method. All specimens from the positive control group showed biofilm formation after 48 h incubation. The mean absorbance value of the control specimens was 1.58 (OD at 492 nm). No evidence of biofilm formation was observed on specimens after the disinfection methods. Disinfection by soaking in 1% sodium hypochlorite and 2% chlorhexidine gluconate and irradiating by microwaves resulted in 100% reduction of MRSA biofilm metabolism. The use of chemical solutions and microwave irradiation was shown to be effective for eradicating mature MRSA biofilms on acrylic resin specimens. |
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Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic SurfacesMRSAacrylic resinsdisinfectionmicrowave irradiationdisinfectantsdenture cleansersMethicillin-resistant Staphylococcus aureus (MRSA) can grow as structured biofilm in different surfaces, including oral mucosa and denture surfaces. Such biofilms can be released into the oral fluids and aspirated, causing systemic infections such as aspiration pneumonia. This study evaluated the efficacy of two disinfectant solutions and microwave irradiation in disinfecting acrylic specimens contaminated with MRSA biofilm. Thirty-six acrylic specimens were made, sterilized and contaminated with MRSA (107 cfu/mL). After incubation (37 °C/48 h), the specimens were divided into 4 groups: not disinfected (positive control); soaking in 1% sodium hypochlorite for 10 min; soaking in 2% chlorhexidine gluconate for 10 min; and irradiating by microwave for 3 min at 650 W. The viability of cells was evaluated by XTT reduction method. All specimens from the positive control group showed biofilm formation after 48 h incubation. The mean absorbance value of the control specimens was 1.58 (OD at 492 nm). No evidence of biofilm formation was observed on specimens after the disinfection methods. Disinfection by soaking in 1% sodium hypochlorite and 2% chlorhexidine gluconate and irradiating by microwaves resulted in 100% reduction of MRSA biofilm metabolism. The use of chemical solutions and microwave irradiation was shown to be effective for eradicating mature MRSA biofilms on acrylic resin specimens.Staphylococcus aureus resistente à meticilina (MRSA, do inglês methicillin-resistant Staphylococcus aureus) pode crescer como biofilme estruturado em diferentes superfícies, incluindo mucosa bucal e superfícies de próteses. Estes biofilmes podem se dispersar nos fluidos orais e ser aspirados, causando infecções sistêmicas, como a pneumonia aspirativa. Este estudo avaliou a eficácia de duas soluções desinfetantes e irradiação por microondas na desinfecção de corpos-de-prova acrílicos contaminados com biofilme de MRSA. Trinta e seis espécimes de resina acrílica foram fabricados, esterilizados e contaminados com MRSA (107 ufc/mL). Após a incubação (37 °C/48 h), os espécimes foram divididos em quatro grupos: não desinfetados (controle positivo); imersos em hipoclorito de sódio 1% por 10 min; imersos em gluconato de clorexidina 2% por 10 min e irradiados por microondas durante 3 min a 650 W. A viabilidade das células foi avaliada pelo método de redução de XTT. Todos os espécimes do grupo controle apresentaram formação de biofilme após 48 h de incubação. O valor médio de absorbância destes espécimes foi de 1.58 (OD a 492 nm). Nenhuma evidência de formação de biofilme foi observada em todas as amostras desinfetadas. A desinfecção em hipoclorito de sódio 1%, gluconato de clorexidina 2% e irradiação em microondas resultou em 100% de redução do metabolismo do biofilme de MRSA. O uso de soluções químicas e irradiação em microondas mostrou-se eficaz na eliminação do biofilme maduro de MRSA sobre corpos-de-prova de resina acrílica.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP - Univ Estadual Paulista Araraquara School of Dentistry Department of Dental Materials and ProsthodonticsUNESP - Univ Estadual Paulista Araraquara School of Dentistry Department of Dental Materials and ProsthodonticsFundação Odontológica de Ribeirão PretoUniversidade Estadual Paulista (Unesp)Altieri, Karen TerezaSanita, Paula VolpatoMachado, Ana LuciaGiampaolo, Eunice TeresinhaPavarina, Ana ClaudiaJorge, Janaina HabibVergani, Carlos Eduardo [UNESP]2015-08-06T16:14:45Z2015-08-06T16:14:45Z2013-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article487-491application/pdfhttp://dx.doi.org/10.1590/0103-6440201302289Brazilian Dental Journal. Fundação Odontológica de Ribeirão Preto, v. 24, n. 5, p. 487-491, 2013.0103-6440http://hdl.handle.net/11449/12618510.1590/0103-6440201302289S0103-64402013000500487S0103-64402013000500487.pdf80002487818425875737127334248549886767053910540330031305224278200000-0002-7375-4714SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2024-01-08T06:21:37Zoai:repositorio.unesp.br:11449/126185Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-08T06:21:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
title |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
spellingShingle |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces Altieri, Karen Tereza MRSA acrylic resins disinfection microwave irradiation disinfectants denture cleansers |
title_short |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
title_full |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
title_fullStr |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
title_full_unstemmed |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
title_sort |
Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces |
author |
Altieri, Karen Tereza |
author_facet |
Altieri, Karen Tereza Sanita, Paula Volpato Machado, Ana Lucia Giampaolo, Eunice Teresinha Pavarina, Ana Claudia Jorge, Janaina Habib Vergani, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
Sanita, Paula Volpato Machado, Ana Lucia Giampaolo, Eunice Teresinha Pavarina, Ana Claudia Jorge, Janaina Habib Vergani, Carlos Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Altieri, Karen Tereza Sanita, Paula Volpato Machado, Ana Lucia Giampaolo, Eunice Teresinha Pavarina, Ana Claudia Jorge, Janaina Habib Vergani, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
MRSA acrylic resins disinfection microwave irradiation disinfectants denture cleansers |
topic |
MRSA acrylic resins disinfection microwave irradiation disinfectants denture cleansers |
description |
Methicillin-resistant Staphylococcus aureus (MRSA) can grow as structured biofilm in different surfaces, including oral mucosa and denture surfaces. Such biofilms can be released into the oral fluids and aspirated, causing systemic infections such as aspiration pneumonia. This study evaluated the efficacy of two disinfectant solutions and microwave irradiation in disinfecting acrylic specimens contaminated with MRSA biofilm. Thirty-six acrylic specimens were made, sterilized and contaminated with MRSA (107 cfu/mL). After incubation (37 °C/48 h), the specimens were divided into 4 groups: not disinfected (positive control); soaking in 1% sodium hypochlorite for 10 min; soaking in 2% chlorhexidine gluconate for 10 min; and irradiating by microwave for 3 min at 650 W. The viability of cells was evaluated by XTT reduction method. All specimens from the positive control group showed biofilm formation after 48 h incubation. The mean absorbance value of the control specimens was 1.58 (OD at 492 nm). No evidence of biofilm formation was observed on specimens after the disinfection methods. Disinfection by soaking in 1% sodium hypochlorite and 2% chlorhexidine gluconate and irradiating by microwaves resulted in 100% reduction of MRSA biofilm metabolism. The use of chemical solutions and microwave irradiation was shown to be effective for eradicating mature MRSA biofilms on acrylic resin specimens. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 2015-08-06T16:14:45Z 2015-08-06T16:14:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201302289 Brazilian Dental Journal. Fundação Odontológica de Ribeirão Preto, v. 24, n. 5, p. 487-491, 2013. 0103-6440 http://hdl.handle.net/11449/126185 10.1590/0103-6440201302289 S0103-64402013000500487 S0103-64402013000500487.pdf 8000248781842587 5737127334248549 8867670539105403 3003130522427820 0000-0002-7375-4714 |
url |
http://dx.doi.org/10.1590/0103-6440201302289 http://hdl.handle.net/11449/126185 |
identifier_str_mv |
Brazilian Dental Journal. Fundação Odontológica de Ribeirão Preto, v. 24, n. 5, p. 487-491, 2013. 0103-6440 10.1590/0103-6440201302289 S0103-64402013000500487 S0103-64402013000500487.pdf 8000248781842587 5737127334248549 8867670539105403 3003130522427820 0000-0002-7375-4714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
487-491 application/pdf |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962283838636032 |