Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/68968 |
Resumo: | The knowledge of cell-cycle control has shown that the capacity of malignant growth is acquired by the stepwise accumulation of defects in specific genes regulating cell growth. Histologic diagnosis might be improved by a quantitative evaluation of more specific diagnosis biomarkers, which could help to precisely identify pre-malignant and malignant oral lesions. The aim of the present study is to evaluate whether computer-based quantitative assessment of p53, PCNA and Ki-67 immunohistochemical expression, could be used clinically to foresee the risk of oral malignant transformation. This retrospective study was carried out in ninety-five oral biopsies, 27 were classified as fibrous inflammatory hyperplasia, 40 as leukoplakia and 28 as oral squamous cell carcinoma. Sixteen out of the 40 leukoplakia were diagnosed as non-dysplastic leukoplakia, the other 24 being dysplastic leukoplakia, of which 50.0% were classified as moderate to severe dysplasia. Comparison of the four groups of oral tissues showed significant rises in p53 and Ki-67 positivity index, which increased steadily in the order benign, pre-malignant, and malignant. In contrast, it was not possible to relate higher PCNA levels with pre-malignant and malignant oral lesions. We therefore conclude that PCNA immunohistochemistry expression is probably an inappropriate marker to identify oral carcinogenesis, whereas joint quantitative evaluation of p53 and Ki-67, appears to be useful as a tumor marker, providing a pre-diagnostic estimate of the potential for cell-cycle deregulation of the oral proliferate status. |
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Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted systemComputer-assisted analysisKi-67Oral cancerp53PCNAcell cycle proteincyclineKi 67 antigenprotein p53tumor markercarcinogenesiscell cycle regulationcomputer assisted diagnosiscomputer systemcontrolled studydiagnostic accuracydisease severityepithelium hyperplasiahistopathologyhumanhuman tissueimmunohistochemistryleukoplakiamajor clinical studymalignant transformationmouth cancerprotein expressionquantitative analysisretrospective studyrisk assessmentsquamous cell carcinomastatistical significanceenzyme immunoassaymetabolismmouth tumorpathologyCarcinoma, Squamous CellCell Cycle ProteinsDiagnosis, Computer-AssistedHumansImmunoenzyme TechniquesKi-67 AntigenLeukoplakia, OralMouth NeoplasmsProliferating Cell Nuclear AntigenRetrospective StudiesTumor Markers, BiologicalTumor Suppressor Protein p53The knowledge of cell-cycle control has shown that the capacity of malignant growth is acquired by the stepwise accumulation of defects in specific genes regulating cell growth. Histologic diagnosis might be improved by a quantitative evaluation of more specific diagnosis biomarkers, which could help to precisely identify pre-malignant and malignant oral lesions. The aim of the present study is to evaluate whether computer-based quantitative assessment of p53, PCNA and Ki-67 immunohistochemical expression, could be used clinically to foresee the risk of oral malignant transformation. This retrospective study was carried out in ninety-five oral biopsies, 27 were classified as fibrous inflammatory hyperplasia, 40 as leukoplakia and 28 as oral squamous cell carcinoma. Sixteen out of the 40 leukoplakia were diagnosed as non-dysplastic leukoplakia, the other 24 being dysplastic leukoplakia, of which 50.0% were classified as moderate to severe dysplasia. Comparison of the four groups of oral tissues showed significant rises in p53 and Ki-67 positivity index, which increased steadily in the order benign, pre-malignant, and malignant. In contrast, it was not possible to relate higher PCNA levels with pre-malignant and malignant oral lesions. We therefore conclude that PCNA immunohistochemistry expression is probably an inappropriate marker to identify oral carcinogenesis, whereas joint quantitative evaluation of p53 and Ki-67, appears to be useful as a tumor marker, providing a pre-diagnostic estimate of the potential for cell-cycle deregulation of the oral proliferate status.Department of Clinical Analysis School of Pharmacy University of São Paulo State (UNESP), Rua Expedicionarios do Brasil 1621, 14 801 902 Araraquara, Sao PauloDepartment of Physiology and Pathology School of Dentistry University of São Paulo State (UNESP), Rua Expedicionarios do Brasil 1621, 14 801 902 Araraquara, Sao PauloDepartment of Pathology Faculty of Medicine of Ribeirão Preto University of São Paulo (USP), Ribeirão Preto, SPDepartment of Clinical Medicine Faculty of Medicine of Ribeirão Preto University of São Paulo (USP), Ribeirão Preto, SPDepartment of Clinical Analysis School of Pharmacy University of São Paulo State (UNESP), Rua Expedicionarios do Brasil 1621, 14 801 902 Araraquara, Sao PauloDepartment of Physiology and Pathology School of Dentistry University of São Paulo State (UNESP), Rua Expedicionarios do Brasil 1621, 14 801 902 Araraquara, Sao PauloUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Soares, C. P. [UNESP]Zuanon, J. A S [UNESP]Teresa, D. B. [UNESP]Fregonezi, P. A. [UNESP]Benatti Neto, Carlos[UNESP]Oliveira, M. R B [UNESP]Donadi, E. A.Martinelli-Kläy, C. P.Soares, E. G.2014-05-27T11:21:54Z2014-05-27T11:21:54Z2006-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article721-728application/pdfhttp://hdl.handle.net/11449/68968Histology and Histopathology, v. 21, n. 7-9, p. 721-728, 2006.0213-3911http://hdl.handle.net/11449/689682-s2.0-336457584852-s2.0-33645758485.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology and Histopathology2.0150,672info:eu-repo/semantics/openAccess2023-11-26T06:16:50Zoai:repositorio.unesp.br:11449/68968Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-26T06:16:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
title |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
spellingShingle |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system Soares, C. P. [UNESP] Computer-assisted analysis Ki-67 Oral cancer p53 PCNA cell cycle protein cycline Ki 67 antigen protein p53 tumor marker carcinogenesis cell cycle regulation computer assisted diagnosis computer system controlled study diagnostic accuracy disease severity epithelium hyperplasia histopathology human human tissue immunohistochemistry leukoplakia major clinical study malignant transformation mouth cancer protein expression quantitative analysis retrospective study risk assessment squamous cell carcinoma statistical significance enzyme immunoassay metabolism mouth tumor pathology Carcinoma, Squamous Cell Cell Cycle Proteins Diagnosis, Computer-Assisted Humans Immunoenzyme Techniques Ki-67 Antigen Leukoplakia, Oral Mouth Neoplasms Proliferating Cell Nuclear Antigen Retrospective Studies Tumor Markers, Biological Tumor Suppressor Protein p53 |
title_short |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
title_full |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
title_fullStr |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
title_full_unstemmed |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
title_sort |
Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system |
author |
Soares, C. P. [UNESP] |
author_facet |
Soares, C. P. [UNESP] Zuanon, J. A S [UNESP] Teresa, D. B. [UNESP] Fregonezi, P. A. [UNESP] Benatti Neto, Carlos[UNESP] Oliveira, M. R B [UNESP] Donadi, E. A. Martinelli-Kläy, C. P. Soares, E. G. |
author_role |
author |
author2 |
Zuanon, J. A S [UNESP] Teresa, D. B. [UNESP] Fregonezi, P. A. [UNESP] Benatti Neto, Carlos[UNESP] Oliveira, M. R B [UNESP] Donadi, E. A. Martinelli-Kläy, C. P. Soares, E. G. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Soares, C. P. [UNESP] Zuanon, J. A S [UNESP] Teresa, D. B. [UNESP] Fregonezi, P. A. [UNESP] Benatti Neto, Carlos[UNESP] Oliveira, M. R B [UNESP] Donadi, E. A. Martinelli-Kläy, C. P. Soares, E. G. |
dc.subject.por.fl_str_mv |
Computer-assisted analysis Ki-67 Oral cancer p53 PCNA cell cycle protein cycline Ki 67 antigen protein p53 tumor marker carcinogenesis cell cycle regulation computer assisted diagnosis computer system controlled study diagnostic accuracy disease severity epithelium hyperplasia histopathology human human tissue immunohistochemistry leukoplakia major clinical study malignant transformation mouth cancer protein expression quantitative analysis retrospective study risk assessment squamous cell carcinoma statistical significance enzyme immunoassay metabolism mouth tumor pathology Carcinoma, Squamous Cell Cell Cycle Proteins Diagnosis, Computer-Assisted Humans Immunoenzyme Techniques Ki-67 Antigen Leukoplakia, Oral Mouth Neoplasms Proliferating Cell Nuclear Antigen Retrospective Studies Tumor Markers, Biological Tumor Suppressor Protein p53 |
topic |
Computer-assisted analysis Ki-67 Oral cancer p53 PCNA cell cycle protein cycline Ki 67 antigen protein p53 tumor marker carcinogenesis cell cycle regulation computer assisted diagnosis computer system controlled study diagnostic accuracy disease severity epithelium hyperplasia histopathology human human tissue immunohistochemistry leukoplakia major clinical study malignant transformation mouth cancer protein expression quantitative analysis retrospective study risk assessment squamous cell carcinoma statistical significance enzyme immunoassay metabolism mouth tumor pathology Carcinoma, Squamous Cell Cell Cycle Proteins Diagnosis, Computer-Assisted Humans Immunoenzyme Techniques Ki-67 Antigen Leukoplakia, Oral Mouth Neoplasms Proliferating Cell Nuclear Antigen Retrospective Studies Tumor Markers, Biological Tumor Suppressor Protein p53 |
description |
The knowledge of cell-cycle control has shown that the capacity of malignant growth is acquired by the stepwise accumulation of defects in specific genes regulating cell growth. Histologic diagnosis might be improved by a quantitative evaluation of more specific diagnosis biomarkers, which could help to precisely identify pre-malignant and malignant oral lesions. The aim of the present study is to evaluate whether computer-based quantitative assessment of p53, PCNA and Ki-67 immunohistochemical expression, could be used clinically to foresee the risk of oral malignant transformation. This retrospective study was carried out in ninety-five oral biopsies, 27 were classified as fibrous inflammatory hyperplasia, 40 as leukoplakia and 28 as oral squamous cell carcinoma. Sixteen out of the 40 leukoplakia were diagnosed as non-dysplastic leukoplakia, the other 24 being dysplastic leukoplakia, of which 50.0% were classified as moderate to severe dysplasia. Comparison of the four groups of oral tissues showed significant rises in p53 and Ki-67 positivity index, which increased steadily in the order benign, pre-malignant, and malignant. In contrast, it was not possible to relate higher PCNA levels with pre-malignant and malignant oral lesions. We therefore conclude that PCNA immunohistochemistry expression is probably an inappropriate marker to identify oral carcinogenesis, whereas joint quantitative evaluation of p53 and Ki-67, appears to be useful as a tumor marker, providing a pre-diagnostic estimate of the potential for cell-cycle deregulation of the oral proliferate status. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-07-01 2014-05-27T11:21:54Z 2014-05-27T11:21:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11449/68968 Histology and Histopathology, v. 21, n. 7-9, p. 721-728, 2006. 0213-3911 http://hdl.handle.net/11449/68968 2-s2.0-33645758485 2-s2.0-33645758485.pdf |
url |
http://hdl.handle.net/11449/68968 |
identifier_str_mv |
Histology and Histopathology, v. 21, n. 7-9, p. 721-728, 2006. 0213-3911 2-s2.0-33645758485 2-s2.0-33645758485.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Histology and Histopathology 2.015 0,672 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
721-728 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965081118703616 |