Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/biom10091235 http://hdl.handle.net/11449/199369 |
Resumo: | Corn cob is an agricultural byproduct that produces an estimated waste burden in the thousands of tons annually, but it is also a good source of xylan, an important bioactive polysaccharide. Silver nanoparticles containing xylan (nanoxylan) were produced using an environmentally friendly synthesis method. To do this, we extracted xylan from corn cobs using an ultrasound technique, which was confirmed by both chemical and NMR analyses. This xylan contained xylose, glucose, arabinose, galactose, mannose, and glucuronic acid in a molar ratio of 50:21:14:9:2.5:2.5, respectively. Nanoxylan synthesis was analyzed using UV–vis spectroscopy at kmax = 469 nm and Fourier transform infrared spectroscopy (FT-IR), which confirmed the presence of both silver and xylan in the nanoxylan product. Dynamic light scattering (DLS) and atomic force microscopy (AFM) revealed that the nanoxylan particles were ~102.0 nm in size and spherical in shape, respectively. DLS also demonstrated that nanoxylan was stable for 12 months and coupled plasma optical emission spectrometry (ICP-OES) showed that the nanoxylan particles were 19% silver. Nanoxylan reduced Leishmania amazonensis promastigote viability with a half maximal inhibitory concentration (IC50) value of 25 µg/mL, while xylan alone showed no effective. Additionally, nanoxylan exhibited antifungal activity against Candida albicans (MIC = 7.5 µg/mL), C. parapsilosis (MIC = 7.5 µg/mL), and Cryptococcus neoformans (MIC = 7.5 µg/mL). Taken together, these data suggest that it is possible to synthesize silver nanoparticles using xylan and that these nanoxylan exert improved antileishmanial and antifungal activities when compared to the untreated polysaccharide or silver nitrate used for their synthesis. Thus, nanoxylan may represent a promising new class of antiparasitic agents for use in the treatment of these microorganisms. |
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Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agentAgricultural byproductAntiparasiticNanomaterialsPolysaccharidesCorn cob is an agricultural byproduct that produces an estimated waste burden in the thousands of tons annually, but it is also a good source of xylan, an important bioactive polysaccharide. Silver nanoparticles containing xylan (nanoxylan) were produced using an environmentally friendly synthesis method. To do this, we extracted xylan from corn cobs using an ultrasound technique, which was confirmed by both chemical and NMR analyses. This xylan contained xylose, glucose, arabinose, galactose, mannose, and glucuronic acid in a molar ratio of 50:21:14:9:2.5:2.5, respectively. Nanoxylan synthesis was analyzed using UV–vis spectroscopy at kmax = 469 nm and Fourier transform infrared spectroscopy (FT-IR), which confirmed the presence of both silver and xylan in the nanoxylan product. Dynamic light scattering (DLS) and atomic force microscopy (AFM) revealed that the nanoxylan particles were ~102.0 nm in size and spherical in shape, respectively. DLS also demonstrated that nanoxylan was stable for 12 months and coupled plasma optical emission spectrometry (ICP-OES) showed that the nanoxylan particles were 19% silver. Nanoxylan reduced Leishmania amazonensis promastigote viability with a half maximal inhibitory concentration (IC50) value of 25 µg/mL, while xylan alone showed no effective. Additionally, nanoxylan exhibited antifungal activity against Candida albicans (MIC = 7.5 µg/mL), C. parapsilosis (MIC = 7.5 µg/mL), and Cryptococcus neoformans (MIC = 7.5 µg/mL). Taken together, these data suggest that it is possible to synthesize silver nanoparticles using xylan and that these nanoxylan exert improved antileishmanial and antifungal activities when compared to the untreated polysaccharide or silver nitrate used for their synthesis. Thus, nanoxylan may represent a promising new class of antiparasitic agents for use in the treatment of these microorganisms.Association for Progressive CommunicationsCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Ministério da Ciência, Tecnologia, Inovações e ComunicaçõesUniversidade Federal do Rio Grande do NorteConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Bioquímica Universidade Federal do Rio Grande do NorteDepartamento de Química Universidade Federal do Rio Grande do NorteCentro de Ciências e Qualidade dos Alimentos (CCQA) Instituto de Tecnologia dos Alimentos (ITAL)Instituto de Biociências Universidade Estadual de São Paulo (Unesp), Praça Infante Dom Henrique, s/nDepartamento de Bioquímica Universidade Federal do ParanáInstituto de Biociências Universidade Estadual de São Paulo (Unesp), Praça Infante Dom Henrique, s/nCNPq: 485813/2012-2Universidade Federal do Rio Grande do NorteInstituto de Tecnologia dos Alimentos (ITAL)Universidade Estadual Paulista (Unesp)Universidade Federal do Paraná (UFPR)Viana, Rony Lucas SilvaFidelis, Gabriel PereiraMedeiros, Mayara Jane CamposMorgano, Marcelo AntonioAlves, Monique Gabriela Chagas FaustinoPassero, Luiz Felipe Domingues [UNESP]Pontes, Daniel LimaTheodoro, Raquel CordeiroArantes, Thales DomingosSabry, Diego AraujoSassaki, Guilherme LanziMelo-Silveira, Raniere FagundesRocha, Hugo Alexandre Oliveira2020-12-12T01:37:58Z2020-12-12T01:37:58Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-21http://dx.doi.org/10.3390/biom10091235Biomolecules, v. 10, n. 9, p. 1-21, 2020.2218-273Xhttp://hdl.handle.net/11449/19936910.3390/biom100912352-s2.0-85090611853Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomoleculesinfo:eu-repo/semantics/openAccess2021-10-22T17:27:45Zoai:repositorio.unesp.br:11449/199369Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T17:27:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
title |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
spellingShingle |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent Viana, Rony Lucas Silva Agricultural byproduct Antiparasitic Nanomaterials Polysaccharides |
title_short |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
title_full |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
title_fullStr |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
title_full_unstemmed |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
title_sort |
Green synthesis of antileishmanial and antifungal silver nanoparticles using corn cob xylan as a reducing and stabilizing agent |
author |
Viana, Rony Lucas Silva |
author_facet |
Viana, Rony Lucas Silva Fidelis, Gabriel Pereira Medeiros, Mayara Jane Campos Morgano, Marcelo Antonio Alves, Monique Gabriela Chagas Faustino Passero, Luiz Felipe Domingues [UNESP] Pontes, Daniel Lima Theodoro, Raquel Cordeiro Arantes, Thales Domingos Sabry, Diego Araujo Sassaki, Guilherme Lanzi Melo-Silveira, Raniere Fagundes Rocha, Hugo Alexandre Oliveira |
author_role |
author |
author2 |
Fidelis, Gabriel Pereira Medeiros, Mayara Jane Campos Morgano, Marcelo Antonio Alves, Monique Gabriela Chagas Faustino Passero, Luiz Felipe Domingues [UNESP] Pontes, Daniel Lima Theodoro, Raquel Cordeiro Arantes, Thales Domingos Sabry, Diego Araujo Sassaki, Guilherme Lanzi Melo-Silveira, Raniere Fagundes Rocha, Hugo Alexandre Oliveira |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte Instituto de Tecnologia dos Alimentos (ITAL) Universidade Estadual Paulista (Unesp) Universidade Federal do Paraná (UFPR) |
dc.contributor.author.fl_str_mv |
Viana, Rony Lucas Silva Fidelis, Gabriel Pereira Medeiros, Mayara Jane Campos Morgano, Marcelo Antonio Alves, Monique Gabriela Chagas Faustino Passero, Luiz Felipe Domingues [UNESP] Pontes, Daniel Lima Theodoro, Raquel Cordeiro Arantes, Thales Domingos Sabry, Diego Araujo Sassaki, Guilherme Lanzi Melo-Silveira, Raniere Fagundes Rocha, Hugo Alexandre Oliveira |
dc.subject.por.fl_str_mv |
Agricultural byproduct Antiparasitic Nanomaterials Polysaccharides |
topic |
Agricultural byproduct Antiparasitic Nanomaterials Polysaccharides |
description |
Corn cob is an agricultural byproduct that produces an estimated waste burden in the thousands of tons annually, but it is also a good source of xylan, an important bioactive polysaccharide. Silver nanoparticles containing xylan (nanoxylan) were produced using an environmentally friendly synthesis method. To do this, we extracted xylan from corn cobs using an ultrasound technique, which was confirmed by both chemical and NMR analyses. This xylan contained xylose, glucose, arabinose, galactose, mannose, and glucuronic acid in a molar ratio of 50:21:14:9:2.5:2.5, respectively. Nanoxylan synthesis was analyzed using UV–vis spectroscopy at kmax = 469 nm and Fourier transform infrared spectroscopy (FT-IR), which confirmed the presence of both silver and xylan in the nanoxylan product. Dynamic light scattering (DLS) and atomic force microscopy (AFM) revealed that the nanoxylan particles were ~102.0 nm in size and spherical in shape, respectively. DLS also demonstrated that nanoxylan was stable for 12 months and coupled plasma optical emission spectrometry (ICP-OES) showed that the nanoxylan particles were 19% silver. Nanoxylan reduced Leishmania amazonensis promastigote viability with a half maximal inhibitory concentration (IC50) value of 25 µg/mL, while xylan alone showed no effective. Additionally, nanoxylan exhibited antifungal activity against Candida albicans (MIC = 7.5 µg/mL), C. parapsilosis (MIC = 7.5 µg/mL), and Cryptococcus neoformans (MIC = 7.5 µg/mL). Taken together, these data suggest that it is possible to synthesize silver nanoparticles using xylan and that these nanoxylan exert improved antileishmanial and antifungal activities when compared to the untreated polysaccharide or silver nitrate used for their synthesis. Thus, nanoxylan may represent a promising new class of antiparasitic agents for use in the treatment of these microorganisms. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:37:58Z 2020-12-12T01:37:58Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/biom10091235 Biomolecules, v. 10, n. 9, p. 1-21, 2020. 2218-273X http://hdl.handle.net/11449/199369 10.3390/biom10091235 2-s2.0-85090611853 |
url |
http://dx.doi.org/10.3390/biom10091235 http://hdl.handle.net/11449/199369 |
identifier_str_mv |
Biomolecules, v. 10, n. 9, p. 1-21, 2020. 2218-273X 10.3390/biom10091235 2-s2.0-85090611853 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-21 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964926553358336 |