Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbi.2015.06.035 http://hdl.handle.net/11449/160814 |
Resumo: | The persistent effects of animal fat consumption during pregnancy and nursing on the programming of breast cancer risk among female offspring were studied here. We have previously found that female offspring of rat dams that consumed a lard-based high-fat (HF) diet (60% fat-derived energy) during pregnancy, or during pregnancy and lactation, were at a reduced risk of developing mammary cancer. To better understand the unexpected protective effects of early life lard exposure, we have applied lipidomics and nutrigenomics approaches to investigate the fatty acid profile and global gene expression patterns in the mammary tissue of the female offspring. Consumption of this HF diet during gestation had few effects on the mammary tissue fatty acids profile of young adult offspring, while exposure from gestation throughout nursing promoted significant alterations in the fatty acids profile. Major differences were related to decreases in saturated fatty acids (SFA) and increases in omega-6 polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFAs) and conjugated linolenic acid (CIA) concentrations. In addition several differences in gene expression patterns by microarray analysis between the control and in utero or in utero and during lactation HF exposed offspring were identified. Differential dependency network (DDN) analysis indicated that many of the genes exhibited unique connections to other genes only in the HF offspring. These unique connections included Hrhl-Ythdf1 and Repinl-Elavl2 in the in utero HF offspring, and Rnf213-Htr3b and Klf5-Chrna4 in the in utero and lactation HF offspring, compared with the control offspring. We conclude that an exposure to a lard-based HF diet during early life changes the fatty acid profile and transcriptional network in mammary gland in young adult rats, and these changes appear to be consistent with reduced mammary cancer risk observed in our previous study. (C) 2015 Elsevier Ireland Ltd. All rights reserved. |
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Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthoodBreast cancerAnimal fatFetal programmingGlobal gene expressionLipidomicDDN analysisThe persistent effects of animal fat consumption during pregnancy and nursing on the programming of breast cancer risk among female offspring were studied here. We have previously found that female offspring of rat dams that consumed a lard-based high-fat (HF) diet (60% fat-derived energy) during pregnancy, or during pregnancy and lactation, were at a reduced risk of developing mammary cancer. To better understand the unexpected protective effects of early life lard exposure, we have applied lipidomics and nutrigenomics approaches to investigate the fatty acid profile and global gene expression patterns in the mammary tissue of the female offspring. Consumption of this HF diet during gestation had few effects on the mammary tissue fatty acids profile of young adult offspring, while exposure from gestation throughout nursing promoted significant alterations in the fatty acids profile. Major differences were related to decreases in saturated fatty acids (SFA) and increases in omega-6 polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFAs) and conjugated linolenic acid (CIA) concentrations. In addition several differences in gene expression patterns by microarray analysis between the control and in utero or in utero and during lactation HF exposed offspring were identified. Differential dependency network (DDN) analysis indicated that many of the genes exhibited unique connections to other genes only in the HF offspring. These unique connections included Hrhl-Ythdf1 and Repinl-Elavl2 in the in utero HF offspring, and Rnf213-Htr3b and Klf5-Chrna4 in the in utero and lactation HF offspring, compared with the control offspring. We conclude that an exposure to a lard-based HF diet during early life changes the fatty acid profile and transcriptional network in mammary gland in young adult rats, and these changes appear to be consistent with reduced mammary cancer risk observed in our previous study. (C) 2015 Elsevier Ireland Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, BR-05508900 Sao Paulo, SP, BrazilGeorgetown Univ, Sch Med, Lombardi Comprehens Canc Ctr, Dept Oncol, Washington, DC 20007 USAState Univ Sao Paulo Botucatu, Botucatu Biosci Inst, Dept Morphol, BR-18618000 Botucatu, SP, BrazilFood Res Ctr NAPAN, BR-05508900 Sao Paulo, SP, BrazilState Univ Sao Paulo Botucatu, Botucatu Biosci Inst, Dept Morphol, BR-18618000 Botucatu, SP, BrazilFAPESP: 2011/23259-4FAPESP: 2010/11742-0FAPESP: 2012/03330-9Elsevier B.V.Universidade de São Paulo (USP)Georgetown UnivUniversidade Estadual Paulista (Unesp)Food Res Ctr NAPANAndrade, Fabia de OliveiraAssis, Sonia deJin, LuFontelles, Camile CastilhoBarbisan, Luis Fernando [UNESP]Purgatto, EduardoHilakivi-Clarke, LeenaOng, Thomas Prates2018-11-26T16:16:51Z2018-11-26T16:16:51Z2015-09-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article118-128application/pdfhttp://dx.doi.org/10.1016/j.cbi.2015.06.035Chemico-biological Interactions. Clare: Elsevier Ireland Ltd, v. 239, p. 118-128, 2015.0009-2797http://hdl.handle.net/11449/16081410.1016/j.cbi.2015.06.035WOS:000361405600013WOS000361405600013.pdf3278528112652257Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemico-biological Interactions1,033info:eu-repo/semantics/openAccess2023-10-16T06:07:16Zoai:repositorio.unesp.br:11449/160814Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-16T06:07:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
title |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
spellingShingle |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood Andrade, Fabia de Oliveira Breast cancer Animal fat Fetal programming Global gene expression Lipidomic DDN analysis |
title_short |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
title_full |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
title_fullStr |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
title_full_unstemmed |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
title_sort |
Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood |
author |
Andrade, Fabia de Oliveira |
author_facet |
Andrade, Fabia de Oliveira Assis, Sonia de Jin, Lu Fontelles, Camile Castilho Barbisan, Luis Fernando [UNESP] Purgatto, Eduardo Hilakivi-Clarke, Leena Ong, Thomas Prates |
author_role |
author |
author2 |
Assis, Sonia de Jin, Lu Fontelles, Camile Castilho Barbisan, Luis Fernando [UNESP] Purgatto, Eduardo Hilakivi-Clarke, Leena Ong, Thomas Prates |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Georgetown Univ Universidade Estadual Paulista (Unesp) Food Res Ctr NAPAN |
dc.contributor.author.fl_str_mv |
Andrade, Fabia de Oliveira Assis, Sonia de Jin, Lu Fontelles, Camile Castilho Barbisan, Luis Fernando [UNESP] Purgatto, Eduardo Hilakivi-Clarke, Leena Ong, Thomas Prates |
dc.subject.por.fl_str_mv |
Breast cancer Animal fat Fetal programming Global gene expression Lipidomic DDN analysis |
topic |
Breast cancer Animal fat Fetal programming Global gene expression Lipidomic DDN analysis |
description |
The persistent effects of animal fat consumption during pregnancy and nursing on the programming of breast cancer risk among female offspring were studied here. We have previously found that female offspring of rat dams that consumed a lard-based high-fat (HF) diet (60% fat-derived energy) during pregnancy, or during pregnancy and lactation, were at a reduced risk of developing mammary cancer. To better understand the unexpected protective effects of early life lard exposure, we have applied lipidomics and nutrigenomics approaches to investigate the fatty acid profile and global gene expression patterns in the mammary tissue of the female offspring. Consumption of this HF diet during gestation had few effects on the mammary tissue fatty acids profile of young adult offspring, while exposure from gestation throughout nursing promoted significant alterations in the fatty acids profile. Major differences were related to decreases in saturated fatty acids (SFA) and increases in omega-6 polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFAs) and conjugated linolenic acid (CIA) concentrations. In addition several differences in gene expression patterns by microarray analysis between the control and in utero or in utero and during lactation HF exposed offspring were identified. Differential dependency network (DDN) analysis indicated that many of the genes exhibited unique connections to other genes only in the HF offspring. These unique connections included Hrhl-Ythdf1 and Repinl-Elavl2 in the in utero HF offspring, and Rnf213-Htr3b and Klf5-Chrna4 in the in utero and lactation HF offspring, compared with the control offspring. We conclude that an exposure to a lard-based HF diet during early life changes the fatty acid profile and transcriptional network in mammary gland in young adult rats, and these changes appear to be consistent with reduced mammary cancer risk observed in our previous study. (C) 2015 Elsevier Ireland Ltd. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-05 2018-11-26T16:16:51Z 2018-11-26T16:16:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbi.2015.06.035 Chemico-biological Interactions. Clare: Elsevier Ireland Ltd, v. 239, p. 118-128, 2015. 0009-2797 http://hdl.handle.net/11449/160814 10.1016/j.cbi.2015.06.035 WOS:000361405600013 WOS000361405600013.pdf 3278528112652257 |
url |
http://dx.doi.org/10.1016/j.cbi.2015.06.035 http://hdl.handle.net/11449/160814 |
identifier_str_mv |
Chemico-biological Interactions. Clare: Elsevier Ireland Ltd, v. 239, p. 118-128, 2015. 0009-2797 10.1016/j.cbi.2015.06.035 WOS:000361405600013 WOS000361405600013.pdf 3278528112652257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemico-biological Interactions 1,033 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
118-128 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964586253746176 |