Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1809-6891v21e-58640 http://hdl.handle.net/11449/205567 |
Resumo: | The aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution. |
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Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissueImunomarcação de metaloproteinases 9 (MMP-9) e 2 (MMP-2) e seus inibidores (TIMP-1) e (TIMP-2) no tecido mamário canino normal e neoplásicoDogMammary glandMatrix metalloproteinasesNeoplasmThe aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution.Universidade Federal de Goiás (UFG)Universidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Ferreira, Hugo HenriqueToledo, Denise CarolineFaria, Adriana MarquesAraújo, Diego Pereiradi Nardi, Andrigo Barboza [UNESP]Porto, Regiani Nascimento Gagnode Moura, Veridiana Maria Brianezi Dignani2021-06-25T10:17:37Z2021-06-25T10:17:37Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1809-6891v21e-58640Ciencia Animal Brasileira, v. 21.1809-68911518-2797http://hdl.handle.net/11449/20556710.1590/1809-6891v21e-58640S1809-689120200001003152-s2.0-85097231603S1809-68912020000100315.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Animal Brasileirainfo:eu-repo/semantics/openAccess2023-10-11T06:02:17Zoai:repositorio.unesp.br:11449/205567Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-11T06:02:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue Imunomarcação de metaloproteinases 9 (MMP-9) e 2 (MMP-2) e seus inibidores (TIMP-1) e (TIMP-2) no tecido mamário canino normal e neoplásico |
title |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
spellingShingle |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue Ferreira, Hugo Henrique Dog Mammary gland Matrix metalloproteinases Neoplasm |
title_short |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_full |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_fullStr |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_full_unstemmed |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_sort |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
author |
Ferreira, Hugo Henrique |
author_facet |
Ferreira, Hugo Henrique Toledo, Denise Caroline Faria, Adriana Marques Araújo, Diego Pereira di Nardi, Andrigo Barboza [UNESP] Porto, Regiani Nascimento Gagno de Moura, Veridiana Maria Brianezi Dignani |
author_role |
author |
author2 |
Toledo, Denise Caroline Faria, Adriana Marques Araújo, Diego Pereira di Nardi, Andrigo Barboza [UNESP] Porto, Regiani Nascimento Gagno de Moura, Veridiana Maria Brianezi Dignani |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferreira, Hugo Henrique Toledo, Denise Caroline Faria, Adriana Marques Araújo, Diego Pereira di Nardi, Andrigo Barboza [UNESP] Porto, Regiani Nascimento Gagno de Moura, Veridiana Maria Brianezi Dignani |
dc.subject.por.fl_str_mv |
Dog Mammary gland Matrix metalloproteinases Neoplasm |
topic |
Dog Mammary gland Matrix metalloproteinases Neoplasm |
description |
The aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T10:17:37Z 2021-06-25T10:17:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1809-6891v21e-58640 Ciencia Animal Brasileira, v. 21. 1809-6891 1518-2797 http://hdl.handle.net/11449/205567 10.1590/1809-6891v21e-58640 S1809-68912020000100315 2-s2.0-85097231603 S1809-68912020000100315.pdf |
url |
http://dx.doi.org/10.1590/1809-6891v21e-58640 http://hdl.handle.net/11449/205567 |
identifier_str_mv |
Ciencia Animal Brasileira, v. 21. 1809-6891 1518-2797 10.1590/1809-6891v21e-58640 S1809-68912020000100315 2-s2.0-85097231603 S1809-68912020000100315.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciencia Animal Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789356088360960 |