Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-017-08790-5 http://hdl.handle.net/11449/163132 |
Resumo: | Preventive infusion of antibiotics in the mammary gland of cows consumes 11 tons/year of medically relevant antimicrobials, yet, this practice might not be critical to prevent new infections in the healthy mammary gland of cows. Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy without antibiotics on milk microbiome and bacterial load, respectively. Cows diagnosed as negative for mastitis at dry off were randomly allocated to receive antibiotic (intramammary ceftiofur hydrochloride) and teat sealant or just teat sealant. Firmicutes was the most abundant phylum, and Corynebacterium, Acinetobacter, and Staphylococcus, often involved in mastitis cases, were the most abundant genera across treatments and time. However, there were no effects of antimicrobial on milk microbiome and bacterial load. Bacterial load was greater at seven days postpartum than at dry off. Dry cow therapy based on teat sealant without antibiotics can be used with no detrimental impacts on milk microbiome and bacterial load in cows with a healthy mammary gland. |
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Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary glandPreventive infusion of antibiotics in the mammary gland of cows consumes 11 tons/year of medically relevant antimicrobials, yet, this practice might not be critical to prevent new infections in the healthy mammary gland of cows. Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy without antibiotics on milk microbiome and bacterial load, respectively. Cows diagnosed as negative for mastitis at dry off were randomly allocated to receive antibiotic (intramammary ceftiofur hydrochloride) and teat sealant or just teat sealant. Firmicutes was the most abundant phylum, and Corynebacterium, Acinetobacter, and Staphylococcus, often involved in mastitis cases, were the most abundant genera across treatments and time. However, there were no effects of antimicrobial on milk microbiome and bacterial load. Bacterial load was greater at seven days postpartum than at dry off. Dry cow therapy based on teat sealant without antibiotics can be used with no detrimental impacts on milk microbiome and bacterial load in cows with a healthy mammary gland.Department of Veterinary Clinical Medicine, College of Veterinary MedicineUniversity of Illinois at Urbana-ChampaignFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Illinois, Dept Vet Clin Med, Champaign, IL 61820 USASao Paulo State Univ, Dept Microbiol & Immunol, Inst Biosci, Botucatu, SP, BrazilCornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY USAUniv Liverpool, Dept Epidemiol & Populat Hlth, Inst Infect & Global Hlth, Leahurst, Neston, EnglandSao Paulo State Univ, Dept Microbiol & Immunol, Inst Biosci, Botucatu, SP, BrazilFAPESP: 2015/152081Nature Publishing GroupUniv IllinoisUniversidade Estadual Paulista (Unesp)Cornell UnivUniv LiverpoolBonsaglia, Erika C. R. [UNESP]Gomes, Marilia S.Canisso, Igor F.Zhou, ZiyaoLima, Svetlana F.Rall, Vera L. M. [UNESP]Oikonomou, GeorgiosBicalho, Rodrigo C.Lima, Fabio S.2018-11-26T17:40:14Z2018-11-26T17:40:14Z2017-08-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1038/s41598-017-08790-5Scientific Reports. London: Nature Publishing Group, v. 7, 10 p., 2017.2045-2322http://hdl.handle.net/11449/16313210.1038/s41598-017-08790-5WOS:000407559800034WOS000407559800034.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-12-07T06:17:36Zoai:repositorio.unesp.br:11449/163132Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-07T06:17:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
title |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
spellingShingle |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland Bonsaglia, Erika C. R. [UNESP] |
title_short |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
title_full |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
title_fullStr |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
title_full_unstemmed |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
title_sort |
Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland |
author |
Bonsaglia, Erika C. R. [UNESP] |
author_facet |
Bonsaglia, Erika C. R. [UNESP] Gomes, Marilia S. Canisso, Igor F. Zhou, Ziyao Lima, Svetlana F. Rall, Vera L. M. [UNESP] Oikonomou, Georgios Bicalho, Rodrigo C. Lima, Fabio S. |
author_role |
author |
author2 |
Gomes, Marilia S. Canisso, Igor F. Zhou, Ziyao Lima, Svetlana F. Rall, Vera L. M. [UNESP] Oikonomou, Georgios Bicalho, Rodrigo C. Lima, Fabio S. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Illinois Universidade Estadual Paulista (Unesp) Cornell Univ Univ Liverpool |
dc.contributor.author.fl_str_mv |
Bonsaglia, Erika C. R. [UNESP] Gomes, Marilia S. Canisso, Igor F. Zhou, Ziyao Lima, Svetlana F. Rall, Vera L. M. [UNESP] Oikonomou, Georgios Bicalho, Rodrigo C. Lima, Fabio S. |
description |
Preventive infusion of antibiotics in the mammary gland of cows consumes 11 tons/year of medically relevant antimicrobials, yet, this practice might not be critical to prevent new infections in the healthy mammary gland of cows. Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy without antibiotics on milk microbiome and bacterial load, respectively. Cows diagnosed as negative for mastitis at dry off were randomly allocated to receive antibiotic (intramammary ceftiofur hydrochloride) and teat sealant or just teat sealant. Firmicutes was the most abundant phylum, and Corynebacterium, Acinetobacter, and Staphylococcus, often involved in mastitis cases, were the most abundant genera across treatments and time. However, there were no effects of antimicrobial on milk microbiome and bacterial load. Bacterial load was greater at seven days postpartum than at dry off. Dry cow therapy based on teat sealant without antibiotics can be used with no detrimental impacts on milk microbiome and bacterial load in cows with a healthy mammary gland. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-14 2018-11-26T17:40:14Z 2018-11-26T17:40:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-017-08790-5 Scientific Reports. London: Nature Publishing Group, v. 7, 10 p., 2017. 2045-2322 http://hdl.handle.net/11449/163132 10.1038/s41598-017-08790-5 WOS:000407559800034 WOS000407559800034.pdf |
url |
http://dx.doi.org/10.1038/s41598-017-08790-5 http://hdl.handle.net/11449/163132 |
identifier_str_mv |
Scientific Reports. London: Nature Publishing Group, v. 7, 10 p., 2017. 2045-2322 10.1038/s41598-017-08790-5 WOS:000407559800034 WOS000407559800034.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965202468306944 |