Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2016.12.034 http://hdl.handle.net/11449/174019 |
Resumo: | Anthelmintic drugs are successfully used in aquaculture to control parasitic infections or infestations. This study analyzed the effectiveness of levamisole as an antiparasitic and its effect on the liver of juvenile pacu (Piaractus mesopotamicus). A total of 300 fish (180 ± 1.27 g; 16 ± 0.4 cm) were fed a diet containing levamisole hydrochloride (LHC) for fifteen days. A control group (T0) and four treatments were tested: T1 (100), T2 (150), T3 (300) and T4 (500) mg kg− 1 LHC in quadruplicate (15 fish per repetition). Four fish per treatment (n = 20) were euthanized by sectioning the spinal cord; the sample was forwarded for parasitological analysis. The gill arches and intestines were removed and preserved in formaldehyde 10% for identification and quantification of the parasites. For evaluation of the liver histology, four fish per treatment (n = 20) were euthanized with benzocaine (100 mg L− 1) for liver removal. In intestines and gills, we identified the parasites Rondonia rondoni (Nematoda, Atractinae) and Anacanthorus penilabiatus (Monogenoidea, Dactylogyridae), respectively. Both were at high prevalence and intensity of infection and infestation, respectively. The 300 mg kg− 1 LHC treatment reduced the infection by R. rondoni but not the infestation by A. penilabiatus. Concentrations of 150 and 300 mg kg− 1 LHC caused moderate liver changes, and no changes were observed in fish treated with 100 mg kg− 1 LHC. The highest concentration of LHC (500 mg kg− 1) induced a high occurrence of sinusoid dilation, blood congestion and leukocyte infiltration in fish liver. Our results indicate that LHC (300 mg kg− 1) was effective in controlling the infection by R. rondoni and caused moderate histological changes in pacu liver. |
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Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus)AnthelminticAquacultureImidotiazolLiver histologyParasitesAnthelmintic drugs are successfully used in aquaculture to control parasitic infections or infestations. This study analyzed the effectiveness of levamisole as an antiparasitic and its effect on the liver of juvenile pacu (Piaractus mesopotamicus). A total of 300 fish (180 ± 1.27 g; 16 ± 0.4 cm) were fed a diet containing levamisole hydrochloride (LHC) for fifteen days. A control group (T0) and four treatments were tested: T1 (100), T2 (150), T3 (300) and T4 (500) mg kg− 1 LHC in quadruplicate (15 fish per repetition). Four fish per treatment (n = 20) were euthanized by sectioning the spinal cord; the sample was forwarded for parasitological analysis. The gill arches and intestines were removed and preserved in formaldehyde 10% for identification and quantification of the parasites. For evaluation of the liver histology, four fish per treatment (n = 20) were euthanized with benzocaine (100 mg L− 1) for liver removal. In intestines and gills, we identified the parasites Rondonia rondoni (Nematoda, Atractinae) and Anacanthorus penilabiatus (Monogenoidea, Dactylogyridae), respectively. Both were at high prevalence and intensity of infection and infestation, respectively. The 300 mg kg− 1 LHC treatment reduced the infection by R. rondoni but not the infestation by A. penilabiatus. Concentrations of 150 and 300 mg kg− 1 LHC caused moderate liver changes, and no changes were observed in fish treated with 100 mg kg− 1 LHC. The highest concentration of LHC (500 mg kg− 1) induced a high occurrence of sinusoid dilation, blood congestion and leukocyte infiltration in fish liver. Our results indicate that LHC (300 mg kg− 1) was effective in controlling the infection by R. rondoni and caused moderate histological changes in pacu liver.Centro de Aquicultura Universidade Estadual Paulista - UNESP (CAUNESP)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – UNESPUniversidade Federal do Rio Grande - FURGCentro de Aquicultura Universidade Estadual Paulista - UNESP (CAUNESP)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – UNESPUniversidade Estadual Paulista (Unesp)Universidade Federal do Rio Grande - FURGPahor-Filho, Eduardo [UNESP]Júnior, Joaber PereiraPilarski, Fabiana [UNESP]Urbinati, Elisabeth Criscuolo [UNESP]2018-12-11T17:08:46Z2018-12-11T17:08:46Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article123-128application/pdfhttp://dx.doi.org/10.1016/j.aquaculture.2016.12.034Aquaculture, v. 470, p. 123-128.0044-8486http://hdl.handle.net/11449/17401910.1016/j.aquaculture.2016.12.0342-s2.0-850076183222-s2.0-85007618322.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture1,152info:eu-repo/semantics/openAccess2024-04-09T15:36:42Zoai:repositorio.unesp.br:11449/174019Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:36:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
title |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
spellingShingle |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) Pahor-Filho, Eduardo [UNESP] Anthelmintic Aquaculture Imidotiazol Liver histology Parasites |
title_short |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
title_full |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
title_fullStr |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
title_full_unstemmed |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
title_sort |
Levamisole reduces parasitic infection in juvenile pacu (Piaractus mesopotamicus) |
author |
Pahor-Filho, Eduardo [UNESP] |
author_facet |
Pahor-Filho, Eduardo [UNESP] Júnior, Joaber Pereira Pilarski, Fabiana [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
author_role |
author |
author2 |
Júnior, Joaber Pereira Pilarski, Fabiana [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Rio Grande - FURG |
dc.contributor.author.fl_str_mv |
Pahor-Filho, Eduardo [UNESP] Júnior, Joaber Pereira Pilarski, Fabiana [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
dc.subject.por.fl_str_mv |
Anthelmintic Aquaculture Imidotiazol Liver histology Parasites |
topic |
Anthelmintic Aquaculture Imidotiazol Liver histology Parasites |
description |
Anthelmintic drugs are successfully used in aquaculture to control parasitic infections or infestations. This study analyzed the effectiveness of levamisole as an antiparasitic and its effect on the liver of juvenile pacu (Piaractus mesopotamicus). A total of 300 fish (180 ± 1.27 g; 16 ± 0.4 cm) were fed a diet containing levamisole hydrochloride (LHC) for fifteen days. A control group (T0) and four treatments were tested: T1 (100), T2 (150), T3 (300) and T4 (500) mg kg− 1 LHC in quadruplicate (15 fish per repetition). Four fish per treatment (n = 20) were euthanized by sectioning the spinal cord; the sample was forwarded for parasitological analysis. The gill arches and intestines were removed and preserved in formaldehyde 10% for identification and quantification of the parasites. For evaluation of the liver histology, four fish per treatment (n = 20) were euthanized with benzocaine (100 mg L− 1) for liver removal. In intestines and gills, we identified the parasites Rondonia rondoni (Nematoda, Atractinae) and Anacanthorus penilabiatus (Monogenoidea, Dactylogyridae), respectively. Both were at high prevalence and intensity of infection and infestation, respectively. The 300 mg kg− 1 LHC treatment reduced the infection by R. rondoni but not the infestation by A. penilabiatus. Concentrations of 150 and 300 mg kg− 1 LHC caused moderate liver changes, and no changes were observed in fish treated with 100 mg kg− 1 LHC. The highest concentration of LHC (500 mg kg− 1) induced a high occurrence of sinusoid dilation, blood congestion and leukocyte infiltration in fish liver. Our results indicate that LHC (300 mg kg− 1) was effective in controlling the infection by R. rondoni and caused moderate histological changes in pacu liver. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:08:46Z 2018-12-11T17:08:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2016.12.034 Aquaculture, v. 470, p. 123-128. 0044-8486 http://hdl.handle.net/11449/174019 10.1016/j.aquaculture.2016.12.034 2-s2.0-85007618322 2-s2.0-85007618322.pdf |
url |
http://dx.doi.org/10.1016/j.aquaculture.2016.12.034 http://hdl.handle.net/11449/174019 |
identifier_str_mv |
Aquaculture, v. 470, p. 123-128. 0044-8486 10.1016/j.aquaculture.2016.12.034 2-s2.0-85007618322 2-s2.0-85007618322.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture 1,152 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
123-128 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789866989191168 |