Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/genes13071263 http://hdl.handle.net/11449/242178 |
Resumo: | Great efforts have been made to preserve manatees. Recently, a hybrid zone was described between Trichechus inunguis (TIN) and the Trichechus manatus manatus (TMM) in the Amazon estuary. Cytogenetic data on these sirenians are limited, despite being fundamental to understanding the hybridization/introgression dynamics and genomic organization in Trichechus. We analyzed the karyotype of TMM, TIN, and two hybrid specimens (“Poque” and “Vitor”) by classical and molecular cytogenetics. G-band analysis revealed that TMM (2n = 48) and TIN (2n = 56) diverge by at least six Robertsonian translocations and a pericentric inversion. Hybrids had 2n = 50, however, with Autosomal Fundamental Number (FNA) = 88 in “Poque” and FNA = 74 in “Vitor”, and chromosomal distinct pairs in heterozygous; additionally, “Vitor” exhibited heteromorphisms and chromosomes whose pairs could not be determined. The U2 snDNA and Histone H3 multi genes are distributed in small clusters along TIN and TMM chromosomes and have transposable Keno and Helitron elements (TEs) in their sequences. The different karyotypes observed among manatee hybrids may indicate that they represent different generations formed by crossing between fertile hybrids and TIN. On the other hand, it is also possible that all hybrids recorded represent F1 and the observed karyotype differences must result from mechanisms of elimination. |
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Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuaryhybridizationkaryotyperepetitive DNAsireniaGreat efforts have been made to preserve manatees. Recently, a hybrid zone was described between Trichechus inunguis (TIN) and the Trichechus manatus manatus (TMM) in the Amazon estuary. Cytogenetic data on these sirenians are limited, despite being fundamental to understanding the hybridization/introgression dynamics and genomic organization in Trichechus. We analyzed the karyotype of TMM, TIN, and two hybrid specimens (“Poque” and “Vitor”) by classical and molecular cytogenetics. G-band analysis revealed that TMM (2n = 48) and TIN (2n = 56) diverge by at least six Robertsonian translocations and a pericentric inversion. Hybrids had 2n = 50, however, with Autosomal Fundamental Number (FNA) = 88 in “Poque” and FNA = 74 in “Vitor”, and chromosomal distinct pairs in heterozygous; additionally, “Vitor” exhibited heteromorphisms and chromosomes whose pairs could not be determined. The U2 snDNA and Histone H3 multi genes are distributed in small clusters along TIN and TMM chromosomes and have transposable Keno and Helitron elements (TEs) in their sequences. The different karyotypes observed among manatee hybrids may indicate that they represent different generations formed by crossing between fertile hybrids and TIN. On the other hand, it is also possible that all hybrids recorded represent F1 and the observed karyotype differences must result from mechanisms of elimination.Laboratório de Citogenética Centro de Estudos Avançados da Biodiversidade Instituto de Ciências Biológicas Universidade Federal do Pará, PAInstituto Federal de Educação Ciência e Tecnologia do Pará, Campus Itaituba, PALaboratório Genômica Integrativa Departamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista, SPUniversidade do Estado do Pará, Campus Tucuruí, PACentro Nacional de Pesquisa e Conservação da Biodiversidade Marinha do Norte Instituto Chico Mendes de Conservação da Biodiversidade, PACentro Nacional de Pesquisa e Conservação de Mamíferos Aquáticos Instituto Chico Mendes de Conservação de Biodiversidade, SPDepartamento de Zoologia Programa de Pós-Graduação em Biologia Animal/PPBA Laboratório de Ecologia Comportamento e Conservação/LECC Universidade Federal de Pernambuco/UFPE, PEGrupo de Pesquisa em Mamíferos Aquáticos Amazônicos Instituto de Desenvolvimento Sustentável Mamirauá, Estrada do Bexiga, AMRede de Pesquisa e Conservação de Sirênios no Estuário Amazônico, APCampus Porto Grande Instituto Federal de Educação Ciência e Tecnologia do Amapá, Rodovia BR 210, Km 103, s/n, Zona Rural, APInstituto de Estudos Costeiros Universidade Federal do Pará, Campus Bragança, PAZoological Park of Santarém ZOOUNAMA Universidade da Amazônia, PALaboratório de Óleos da Amazônia Universidade Federal do Pará, PADepartamento de Biologia Estrutural Molecular e Genética Universidade Estadual de Ponta Grossa, PRLaboratório Genômica Integrativa Departamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista, SPUniversidade Federal do Pará (UFPA)Ciência e Tecnologia do ParáUniversidade Estadual Paulista (UNESP)Universidade do Estado do ParáInstituto Chico Mendes de Conservação da BiodiversidadeInstituto Chico Mendes de Conservação de BiodiversidadeUniversidade Federal de Pernambuco (UFPE)Instituto de Desenvolvimento Sustentável MamirauáRede de Pesquisa e Conservação de Sirênios no Estuário AmazônicoInstituto Federal de Educação Ciência e Tecnologia do AmapáUniversidade da AmazôniaUniversidade Estadual de Ponta Grossa (UEPG)Noronha, Renata C. R.Almeida, Bruno R. R.Chagas, Monique C. S.Tavares, Flávia S.Cardoso, Adauto L. [UNESP]Bastos, Carlos E. M. C.Silva, Natalia K. N.Klautau, Alex G. C. M.Luna, Fábia O.Attademo, Fernanda L. N.Lima, Danielle S.Sabioni, Luiz A.Sampaio, Maria I. C.Oliveira, Jairo MouraNascimento, Luís Adriano Santos doMartins, Cesar [UNESP]Vicari, Marcelo R.Nagamachi, Cleusa Y.Pieczarka, Julio C.2023-03-02T11:27:36Z2023-03-02T11:27:36Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/genes13071263Genes, v. 13, n. 7, 2022.2073-4425http://hdl.handle.net/11449/24217810.3390/genes130712632-s2.0-85136298256Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2023-03-02T11:27:36Zoai:repositorio.unesp.br:11449/242178Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T11:27:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
title |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
spellingShingle |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary Noronha, Renata C. R. hybridization karyotype repetitive DNA sirenia |
title_short |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
title_full |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
title_fullStr |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
title_full_unstemmed |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
title_sort |
Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary |
author |
Noronha, Renata C. R. |
author_facet |
Noronha, Renata C. R. Almeida, Bruno R. R. Chagas, Monique C. S. Tavares, Flávia S. Cardoso, Adauto L. [UNESP] Bastos, Carlos E. M. C. Silva, Natalia K. N. Klautau, Alex G. C. M. Luna, Fábia O. Attademo, Fernanda L. N. Lima, Danielle S. Sabioni, Luiz A. Sampaio, Maria I. C. Oliveira, Jairo Moura Nascimento, Luís Adriano Santos do Martins, Cesar [UNESP] Vicari, Marcelo R. Nagamachi, Cleusa Y. Pieczarka, Julio C. |
author_role |
author |
author2 |
Almeida, Bruno R. R. Chagas, Monique C. S. Tavares, Flávia S. Cardoso, Adauto L. [UNESP] Bastos, Carlos E. M. C. Silva, Natalia K. N. Klautau, Alex G. C. M. Luna, Fábia O. Attademo, Fernanda L. N. Lima, Danielle S. Sabioni, Luiz A. Sampaio, Maria I. C. Oliveira, Jairo Moura Nascimento, Luís Adriano Santos do Martins, Cesar [UNESP] Vicari, Marcelo R. Nagamachi, Cleusa Y. Pieczarka, Julio C. |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Pará (UFPA) Ciência e Tecnologia do Pará Universidade Estadual Paulista (UNESP) Universidade do Estado do Pará Instituto Chico Mendes de Conservação da Biodiversidade Instituto Chico Mendes de Conservação de Biodiversidade Universidade Federal de Pernambuco (UFPE) Instituto de Desenvolvimento Sustentável Mamirauá Rede de Pesquisa e Conservação de Sirênios no Estuário Amazônico Instituto Federal de Educação Ciência e Tecnologia do Amapá Universidade da Amazônia Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Noronha, Renata C. R. Almeida, Bruno R. R. Chagas, Monique C. S. Tavares, Flávia S. Cardoso, Adauto L. [UNESP] Bastos, Carlos E. M. C. Silva, Natalia K. N. Klautau, Alex G. C. M. Luna, Fábia O. Attademo, Fernanda L. N. Lima, Danielle S. Sabioni, Luiz A. Sampaio, Maria I. C. Oliveira, Jairo Moura Nascimento, Luís Adriano Santos do Martins, Cesar [UNESP] Vicari, Marcelo R. Nagamachi, Cleusa Y. Pieczarka, Julio C. |
dc.subject.por.fl_str_mv |
hybridization karyotype repetitive DNA sirenia |
topic |
hybridization karyotype repetitive DNA sirenia |
description |
Great efforts have been made to preserve manatees. Recently, a hybrid zone was described between Trichechus inunguis (TIN) and the Trichechus manatus manatus (TMM) in the Amazon estuary. Cytogenetic data on these sirenians are limited, despite being fundamental to understanding the hybridization/introgression dynamics and genomic organization in Trichechus. We analyzed the karyotype of TMM, TIN, and two hybrid specimens (“Poque” and “Vitor”) by classical and molecular cytogenetics. G-band analysis revealed that TMM (2n = 48) and TIN (2n = 56) diverge by at least six Robertsonian translocations and a pericentric inversion. Hybrids had 2n = 50, however, with Autosomal Fundamental Number (FNA) = 88 in “Poque” and FNA = 74 in “Vitor”, and chromosomal distinct pairs in heterozygous; additionally, “Vitor” exhibited heteromorphisms and chromosomes whose pairs could not be determined. The U2 snDNA and Histone H3 multi genes are distributed in small clusters along TIN and TMM chromosomes and have transposable Keno and Helitron elements (TEs) in their sequences. The different karyotypes observed among manatee hybrids may indicate that they represent different generations formed by crossing between fertile hybrids and TIN. On the other hand, it is also possible that all hybrids recorded represent F1 and the observed karyotype differences must result from mechanisms of elimination. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-02T11:27:36Z 2023-03-02T11:27:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/genes13071263 Genes, v. 13, n. 7, 2022. 2073-4425 http://hdl.handle.net/11449/242178 10.3390/genes13071263 2-s2.0-85136298256 |
url |
http://dx.doi.org/10.3390/genes13071263 http://hdl.handle.net/11449/242178 |
identifier_str_mv |
Genes, v. 13, n. 7, 2022. 2073-4425 10.3390/genes13071263 2-s2.0-85136298256 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790252035735552 |