Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/biom10040590 http://hdl.handle.net/11449/201689 |
Resumo: | Machaerium hirtum (Vell.) Stellfeld (Fabaceae) known in Brazil as “jacaranda de espinho” or “espinheira santa nativa” is a medicinal plant commonly used in folk medicine to treat ulcers, cough and diarrhea. This study aimed to investigate the anti-inflammatory and antinociceptive effects of hydroalcoholic extracts from M. hirtum twig (HEMh) using in vivo experimental models of nociception through the involvement of transient receptor potential channels, acid-sensing ion channel (ASIC), nitrergic, opioidergic, glutamatergic, and supraspinal pathways. Our results revealed an antinociceptive effect of HEMh mediated by the opioidergic, L-arginine-nitric oxide and glutamate systems, as well as by interactions with TRPA1/ASIC channels. The anti-inflammatory effect of HEMh evaluated with a xylene-induced ear edema and by the involvement of arachidonic acid and prostaglandin E2 (PGE2) showed involvement of the COX pathway, based on observed decreases in PGE2 levels. A phytochemical investigation of the HEMh led to the isolation of α- amyrin, β-amyrin, allantoin, apigenin-7-methoxy-6-C-β-D-glucopyranoside, and apigenin-6-C-β-Dglucopyranosyl- 8-C-β-D-xylopyranoside. In conclusion, the acute oral administration of HEMh inhibits the nociceptive behavioral response in animals through the nitrergic, opioid, glutamatergic pathways, and by inhibition of the TRPA1 and ASIC channels, without causing locomotor dysfunction. In addition, its anti-inflammatory effect is associated with the COX pathway and decreased PGE2 levels. |
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Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of actionAnti-inflammatory actionAntinociceptive effectMachaerium hirtumMachaerium hirtum (Vell.) Stellfeld (Fabaceae) known in Brazil as “jacaranda de espinho” or “espinheira santa nativa” is a medicinal plant commonly used in folk medicine to treat ulcers, cough and diarrhea. This study aimed to investigate the anti-inflammatory and antinociceptive effects of hydroalcoholic extracts from M. hirtum twig (HEMh) using in vivo experimental models of nociception through the involvement of transient receptor potential channels, acid-sensing ion channel (ASIC), nitrergic, opioidergic, glutamatergic, and supraspinal pathways. Our results revealed an antinociceptive effect of HEMh mediated by the opioidergic, L-arginine-nitric oxide and glutamate systems, as well as by interactions with TRPA1/ASIC channels. The anti-inflammatory effect of HEMh evaluated with a xylene-induced ear edema and by the involvement of arachidonic acid and prostaglandin E2 (PGE2) showed involvement of the COX pathway, based on observed decreases in PGE2 levels. A phytochemical investigation of the HEMh led to the isolation of α- amyrin, β-amyrin, allantoin, apigenin-7-methoxy-6-C-β-D-glucopyranoside, and apigenin-6-C-β-Dglucopyranosyl- 8-C-β-D-xylopyranoside. In conclusion, the acute oral administration of HEMh inhibits the nociceptive behavioral response in animals through the nitrergic, opioid, glutamatergic pathways, and by inhibition of the TRPA1 and ASIC channels, without causing locomotor dysfunction. In addition, its anti-inflammatory effect is associated with the COX pathway and decreased PGE2 levels.Department of Structural and Functional Biology Physiology São Paulo State University UNESP Institute of BiosciencesCoastal Campus São Paulo State University UNESPDepartment of Structural and Functional Biology Institute of Biology State University of Campinas UNICAMPBotany Department São Paulo State University UNESP Biosciences InstituteLaboratory of Neurobiology of Pain and Inflammation Department of Physiological Sciences Central of Biological Sciences Federal University of Santa Catarina Trindade FlorianopolisSchool of Arts Sciences and Humanities São Paulo University USPDepartment of Structural and Functional Biology Physiology São Paulo State University UNESP Institute of BiosciencesCoastal Campus São Paulo State University UNESPBotany Department São Paulo State University UNESP Biosciences InstituteUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade Federal de Santa Catarina (UFSC)Universidade de São Paulo (USP)Agostinho Lopes, Juliana [UNESP]Peixoto Rodrigues, Vinícius [UNESP]Marucci Pereira Tangerina, Marcelo [UNESP]Machado Da Rocha, Lucia Regina [UNESP]Massucato Nishijima, CatarineAlfieri Nunes, Vania Vasti [UNESP]Rolim De Almeida, Luiz Fernando [UNESP]Vilegas, Wagner [UNESP]Soares Dos Santos, Adair RobertoSannomiya, MiriamHiruma-Lima, Clélia Akiko [UNESP]2020-12-12T02:39:13Z2020-12-12T02:39:13Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/biom10040590Biomolecules, v. 10, n. 4, 2020.2218-273Xhttp://hdl.handle.net/11449/20168910.3390/biom100405902-s2.0-8508341718638145049013868440000-0002-8645-3777Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomoleculesinfo:eu-repo/semantics/openAccess2022-02-09T00:42:28Zoai:repositorio.unesp.br:11449/201689Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-02-09T00:42:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
title |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
spellingShingle |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action Agostinho Lopes, Juliana [UNESP] Anti-inflammatory action Antinociceptive effect Machaerium hirtum |
title_short |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
title_full |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
title_fullStr |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
title_full_unstemmed |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
title_sort |
Machaerium hirtum (Vell.) stellfeld alleviates acute pain and inflammation: Potential mechanisms of action |
author |
Agostinho Lopes, Juliana [UNESP] |
author_facet |
Agostinho Lopes, Juliana [UNESP] Peixoto Rodrigues, Vinícius [UNESP] Marucci Pereira Tangerina, Marcelo [UNESP] Machado Da Rocha, Lucia Regina [UNESP] Massucato Nishijima, Catarine Alfieri Nunes, Vania Vasti [UNESP] Rolim De Almeida, Luiz Fernando [UNESP] Vilegas, Wagner [UNESP] Soares Dos Santos, Adair Roberto Sannomiya, Miriam Hiruma-Lima, Clélia Akiko [UNESP] |
author_role |
author |
author2 |
Peixoto Rodrigues, Vinícius [UNESP] Marucci Pereira Tangerina, Marcelo [UNESP] Machado Da Rocha, Lucia Regina [UNESP] Massucato Nishijima, Catarine Alfieri Nunes, Vania Vasti [UNESP] Rolim De Almeida, Luiz Fernando [UNESP] Vilegas, Wagner [UNESP] Soares Dos Santos, Adair Roberto Sannomiya, Miriam Hiruma-Lima, Clélia Akiko [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade Federal de Santa Catarina (UFSC) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Agostinho Lopes, Juliana [UNESP] Peixoto Rodrigues, Vinícius [UNESP] Marucci Pereira Tangerina, Marcelo [UNESP] Machado Da Rocha, Lucia Regina [UNESP] Massucato Nishijima, Catarine Alfieri Nunes, Vania Vasti [UNESP] Rolim De Almeida, Luiz Fernando [UNESP] Vilegas, Wagner [UNESP] Soares Dos Santos, Adair Roberto Sannomiya, Miriam Hiruma-Lima, Clélia Akiko [UNESP] |
dc.subject.por.fl_str_mv |
Anti-inflammatory action Antinociceptive effect Machaerium hirtum |
topic |
Anti-inflammatory action Antinociceptive effect Machaerium hirtum |
description |
Machaerium hirtum (Vell.) Stellfeld (Fabaceae) known in Brazil as “jacaranda de espinho” or “espinheira santa nativa” is a medicinal plant commonly used in folk medicine to treat ulcers, cough and diarrhea. This study aimed to investigate the anti-inflammatory and antinociceptive effects of hydroalcoholic extracts from M. hirtum twig (HEMh) using in vivo experimental models of nociception through the involvement of transient receptor potential channels, acid-sensing ion channel (ASIC), nitrergic, opioidergic, glutamatergic, and supraspinal pathways. Our results revealed an antinociceptive effect of HEMh mediated by the opioidergic, L-arginine-nitric oxide and glutamate systems, as well as by interactions with TRPA1/ASIC channels. The anti-inflammatory effect of HEMh evaluated with a xylene-induced ear edema and by the involvement of arachidonic acid and prostaglandin E2 (PGE2) showed involvement of the COX pathway, based on observed decreases in PGE2 levels. A phytochemical investigation of the HEMh led to the isolation of α- amyrin, β-amyrin, allantoin, apigenin-7-methoxy-6-C-β-D-glucopyranoside, and apigenin-6-C-β-Dglucopyranosyl- 8-C-β-D-xylopyranoside. In conclusion, the acute oral administration of HEMh inhibits the nociceptive behavioral response in animals through the nitrergic, opioid, glutamatergic pathways, and by inhibition of the TRPA1 and ASIC channels, without causing locomotor dysfunction. In addition, its anti-inflammatory effect is associated with the COX pathway and decreased PGE2 levels. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:39:13Z 2020-12-12T02:39:13Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/biom10040590 Biomolecules, v. 10, n. 4, 2020. 2218-273X http://hdl.handle.net/11449/201689 10.3390/biom10040590 2-s2.0-85083417186 3814504901386844 0000-0002-8645-3777 |
url |
http://dx.doi.org/10.3390/biom10040590 http://hdl.handle.net/11449/201689 |
identifier_str_mv |
Biomolecules, v. 10, n. 4, 2020. 2218-273X 10.3390/biom10040590 2-s2.0-85083417186 3814504901386844 0000-0002-8645-3777 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789966459207680 |