Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
Main Author: | |
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Publication Date: | 2008 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/S1678-77572008000500003 http://hdl.handle.net/11449/15103 |
Summary: | From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. |
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Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic ratsBone transplantationDiabetes mellitusWound healingFrom a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)State Univ São Paulo, Dent Sch Aracatuba, Dept Surg & Integrate Clin, Aracatuba, SP, BrazilState Univ São Paulo, Dent Sch Aracatuba, Dept Surg & Integrate Clin, Aracatuba, SP, BrazilFAPESP: 04/13990-0Universidade de São Paulo (USP), Faculdade de Odontologia de BauruUniversidade Estadual Paulista (Unesp)Esteves, Jonatas Caldeira [UNESP]Aranega, Alessandra Marcondes [UNESP]Borrasca, Albanir Gabriel [UNESP]Ruiz de Souza Fattah, Cristiane Mara [UNESP]Garcia-Junior, Idelmo Rangel [UNESP]2013-09-30T18:29:34Z2014-05-20T13:43:20Z2013-09-30T18:29:34Z2014-05-20T13:43:20Z2008-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article316-320application/pdfhttp://dx.doi.org/10.1590/S1678-77572008000500003Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008.1678-7757http://hdl.handle.net/11449/15103S1678-77572008000500003WOS:000266688400003S1678-77572008000500003-en.pdf6636749858940359Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-09-19T13:29:56Zoai:repositorio.unesp.br:11449/15103Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T13:29:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
spellingShingle |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats Esteves, Jonatas Caldeira [UNESP] Bone transplantation Diabetes mellitus Wound healing |
title_short |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_full |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_fullStr |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_full_unstemmed |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_sort |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
author |
Esteves, Jonatas Caldeira [UNESP] |
author_facet |
Esteves, Jonatas Caldeira [UNESP] Aranega, Alessandra Marcondes [UNESP] Borrasca, Albanir Gabriel [UNESP] Ruiz de Souza Fattah, Cristiane Mara [UNESP] Garcia-Junior, Idelmo Rangel [UNESP] |
author_role |
author |
author2 |
Aranega, Alessandra Marcondes [UNESP] Borrasca, Albanir Gabriel [UNESP] Ruiz de Souza Fattah, Cristiane Mara [UNESP] Garcia-Junior, Idelmo Rangel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Esteves, Jonatas Caldeira [UNESP] Aranega, Alessandra Marcondes [UNESP] Borrasca, Albanir Gabriel [UNESP] Ruiz de Souza Fattah, Cristiane Mara [UNESP] Garcia-Junior, Idelmo Rangel [UNESP] |
dc.subject.por.fl_str_mv |
Bone transplantation Diabetes mellitus Wound healing |
topic |
Bone transplantation Diabetes mellitus Wound healing |
description |
From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 2013-09-30T18:29:34Z 2013-09-30T18:29:34Z 2014-05-20T13:43:20Z 2014-05-20T13:43:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572008000500003 Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008. 1678-7757 http://hdl.handle.net/11449/15103 S1678-77572008000500003 WOS:000266688400003 S1678-77572008000500003-en.pdf 6636749858940359 |
url |
http://dx.doi.org/10.1590/S1678-77572008000500003 http://hdl.handle.net/11449/15103 |
identifier_str_mv |
Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008. 1678-7757 S1678-77572008000500003 WOS:000266688400003 S1678-77572008000500003-en.pdf 6636749858940359 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science 1.709 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
316-320 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546379570577408 |