Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats

Bibliographic Details
Main Author: Esteves, Jonatas Caldeira [UNESP]
Publication Date: 2008
Other Authors: Aranega, Alessandra Marcondes [UNESP], Borrasca, Albanir Gabriel [UNESP], Ruiz de Souza Fattah, Cristiane Mara [UNESP], Garcia-Junior, Idelmo Rangel [UNESP]
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://dx.doi.org/10.1590/S1678-77572008000500003
http://hdl.handle.net/11449/15103
Summary: From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.
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spelling Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic ratsBone transplantationDiabetes mellitusWound healingFrom a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)State Univ São Paulo, Dent Sch Aracatuba, Dept Surg & Integrate Clin, Aracatuba, SP, BrazilState Univ São Paulo, Dent Sch Aracatuba, Dept Surg & Integrate Clin, Aracatuba, SP, BrazilFAPESP: 04/13990-0Universidade de São Paulo (USP), Faculdade de Odontologia de BauruUniversidade Estadual Paulista (Unesp)Esteves, Jonatas Caldeira [UNESP]Aranega, Alessandra Marcondes [UNESP]Borrasca, Albanir Gabriel [UNESP]Ruiz de Souza Fattah, Cristiane Mara [UNESP]Garcia-Junior, Idelmo Rangel [UNESP]2013-09-30T18:29:34Z2014-05-20T13:43:20Z2013-09-30T18:29:34Z2014-05-20T13:43:20Z2008-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article316-320application/pdfhttp://dx.doi.org/10.1590/S1678-77572008000500003Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008.1678-7757http://hdl.handle.net/11449/15103S1678-77572008000500003WOS:000266688400003S1678-77572008000500003-en.pdf6636749858940359Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-09-19T13:29:56Zoai:repositorio.unesp.br:11449/15103Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T13:29:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
title Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
spellingShingle Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
Esteves, Jonatas Caldeira [UNESP]
Bone transplantation
Diabetes mellitus
Wound healing
title_short Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
title_full Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
title_fullStr Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
title_full_unstemmed Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
title_sort Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
author Esteves, Jonatas Caldeira [UNESP]
author_facet Esteves, Jonatas Caldeira [UNESP]
Aranega, Alessandra Marcondes [UNESP]
Borrasca, Albanir Gabriel [UNESP]
Ruiz de Souza Fattah, Cristiane Mara [UNESP]
Garcia-Junior, Idelmo Rangel [UNESP]
author_role author
author2 Aranega, Alessandra Marcondes [UNESP]
Borrasca, Albanir Gabriel [UNESP]
Ruiz de Souza Fattah, Cristiane Mara [UNESP]
Garcia-Junior, Idelmo Rangel [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Esteves, Jonatas Caldeira [UNESP]
Aranega, Alessandra Marcondes [UNESP]
Borrasca, Albanir Gabriel [UNESP]
Ruiz de Souza Fattah, Cristiane Mara [UNESP]
Garcia-Junior, Idelmo Rangel [UNESP]
dc.subject.por.fl_str_mv Bone transplantation
Diabetes mellitus
Wound healing
topic Bone transplantation
Diabetes mellitus
Wound healing
description From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.
publishDate 2008
dc.date.none.fl_str_mv 2008-09-01
2013-09-30T18:29:34Z
2013-09-30T18:29:34Z
2014-05-20T13:43:20Z
2014-05-20T13:43:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-77572008000500003
Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008.
1678-7757
http://hdl.handle.net/11449/15103
S1678-77572008000500003
WOS:000266688400003
S1678-77572008000500003-en.pdf
6636749858940359
url http://dx.doi.org/10.1590/S1678-77572008000500003
http://hdl.handle.net/11449/15103
identifier_str_mv Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008.
1678-7757
S1678-77572008000500003
WOS:000266688400003
S1678-77572008000500003-en.pdf
6636749858940359
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
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0,645
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 316-320
application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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