Properties of Polyhydroxyalkanoate Granules and Bioemulsifiers from Pseudomonas sp. and Burkholderia sp. Isolates Growing on Glucose

• Main Author: Sacco, Laís Postai [UNESP]
• Publication Date: 2016
• Other Authors: Castellane, Tereza Cristina Luque [UNESP], Lopes, Erica Mendes [UNESP], de Macedo Lemos, Eliana Gertrudes [UNESP], Alves, Lúcia Maria Carareto [UNESP]
• Format: Article
• Language: eng
• Source: Repositório Institucional da UNESP
• Download full: http://dx.doi.org/10.1007/s12010-015-1923-5; http://hdl.handle.net/11449/172217
• topic: Bioemulsifier; Biopolymers; Burkholderia sp; Emulsifying activity; Polyhydroxybutyrate; Pseudomonas sp

A Burkholderia and Pseudomonas species designated as AB4 and AS1, respectively, were isolated from soil containing decomposing straw or sugar cane bagasse collected from Brazil. This study sought to evaluate the capacities of culture media, cell-free medium, and crude lysate preparations (containing PHB inclusion bodies) from bacterial cell cultures to stabilize emulsions with several hydrophobic compounds. Four conditions showed good production of bioemulsifiers (E24 ≥ 50 %), headed by substantially cell-free media from bacterial cell cultures in which bacterial isolates from Burkholderia sp. strain AB4 and Pseudomonas sp. strain AS1 were grown. Our results revealed that the both isolates (AB4 and AS1 strains) exhibited high emulsification indices (indicating usefulness in bioremediation) and good stabilities.