Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/228031 |
Resumo: | Amphotericin B (AmB) is drug “gold standard” for the treatment of invasive fungal infections since 1960. However, amphotericin B has high toxicity, which manifests itself most often in the kidneys and in the liver. It is known, since 1985, that self-oxidation of AmB gives different forms of reactive oxidative species and these, being toxic to the cell, would be responsible, in part, by its toxicity. Different studies indicate that hesperidin contributes, through the reduction of oxidative stress, to protect against renal injury generated by ischemia. This fact and the involvement of AmB in the generation of free radicals make it interesting the preliminary evaluation of the effect of hesperidin and AmB (alone or associated) against reactive oxygen species and free radicals, as well as the study on models of cytotoxicity. Front ABTS•+, AmB presented IC<inf>50</inf> equal to 0.0124 mg/mL, but when it was associated to hesperidin this value has decreased to 0.0003 mg/mL. Front HOCl, Amb presented IC<inf>50</inf> equal to 0.0056, but when it was associated to hesperidin this value has decreased to 0.0023 mg/mL. In the trials with DPPH• and the superoxide anion radical samples were not effective. In the assay with endotelial cell culture (HUVEC cells), the association has decreased cell viability. These preliminary results demonstrate the interaction of the compounds with reactive species as well as indicate the possibility of damage exacerbation by AmB in the presence of antioxidants in an in vitro model. |
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Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvecPreliminary assessment of the association between amphotericin and hesperetin with oxididants and huvec cellsAmphotericin BCytotoxicityHesperidinOxidative stressAmphotericin B (AmB) is drug “gold standard” for the treatment of invasive fungal infections since 1960. However, amphotericin B has high toxicity, which manifests itself most often in the kidneys and in the liver. It is known, since 1985, that self-oxidation of AmB gives different forms of reactive oxidative species and these, being toxic to the cell, would be responsible, in part, by its toxicity. Different studies indicate that hesperidin contributes, through the reduction of oxidative stress, to protect against renal injury generated by ischemia. This fact and the involvement of AmB in the generation of free radicals make it interesting the preliminary evaluation of the effect of hesperidin and AmB (alone or associated) against reactive oxygen species and free radicals, as well as the study on models of cytotoxicity. Front ABTS•+, AmB presented IC<inf>50</inf> equal to 0.0124 mg/mL, but when it was associated to hesperidin this value has decreased to 0.0003 mg/mL. Front HOCl, Amb presented IC<inf>50</inf> equal to 0.0056, but when it was associated to hesperidin this value has decreased to 0.0023 mg/mL. In the trials with DPPH• and the superoxide anion radical samples were not effective. In the assay with endotelial cell culture (HUVEC cells), the association has decreased cell viability. These preliminary results demonstrate the interaction of the compounds with reactive species as well as indicate the possibility of damage exacerbation by AmB in the presence of antioxidants in an in vitro model.Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Departamento de Análises Clínicas, Laboratório de Imunologia ClínicaUniversidade Estadual de Ponta GrossaDepartamento de Análises Clínicas e Toxicológicas, Laboratório de Análises Moleculares e Bioquímicas, Setor de Ciencias Biológicas e da SaúdeUniversidade Estadual Paulista ‘Júlio de Mesquita Filho’, Departamento de Análises Clínicas, Laboratório de Imunologia ClínicaUniversidade Estadual Paulista (UNESP)Universidade Estadual de Ponta Grossa (UEPG)Manente, Francine Alessandra [UNESP]da Silva, Ana Flavia Tostes [UNESP]Schemberger, Josiane Aparecida [UNESP]Heinrich, Isabella Aparecida [UNESP]Munhoz, Antonio Carlos Mattar [UNESP]Beltrame, Flávio Luis [UNESP]Maia, Danielle Cardoso Geraldo [UNESP]Urbaczek, Ana Carolina [UNESP]Carlos, Iracilda Zeppone [UNESP]Vellosa, José Carlos Rebuglio2022-04-29T07:26:24Z2022-04-29T07:26:24Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article679-685Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 35, n. 4, p. 679-685, 2014.1808-4532http://hdl.handle.net/11449/2280312-s2.0-84939503542Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista de Ciencias Farmaceuticas Basica e Aplicadainfo:eu-repo/semantics/openAccess2022-04-29T07:26:24Zoai:repositorio.unesp.br:11449/228031Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:26:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec Preliminary assessment of the association between amphotericin and hesperetin with oxididants and huvec cells |
title |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec |
spellingShingle |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec Manente, Francine Alessandra [UNESP] Amphotericin B Cytotoxicity Hesperidin Oxidative stress |
title_short |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec |
title_full |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec |
title_fullStr |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec |
title_full_unstemmed |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec |
title_sort |
Avaliação preliminar da associação entre anfotericina e hesperina com oxidantes e células huvec |
author |
Manente, Francine Alessandra [UNESP] |
author_facet |
Manente, Francine Alessandra [UNESP] da Silva, Ana Flavia Tostes [UNESP] Schemberger, Josiane Aparecida [UNESP] Heinrich, Isabella Aparecida [UNESP] Munhoz, Antonio Carlos Mattar [UNESP] Beltrame, Flávio Luis [UNESP] Maia, Danielle Cardoso Geraldo [UNESP] Urbaczek, Ana Carolina [UNESP] Carlos, Iracilda Zeppone [UNESP] Vellosa, José Carlos Rebuglio |
author_role |
author |
author2 |
da Silva, Ana Flavia Tostes [UNESP] Schemberger, Josiane Aparecida [UNESP] Heinrich, Isabella Aparecida [UNESP] Munhoz, Antonio Carlos Mattar [UNESP] Beltrame, Flávio Luis [UNESP] Maia, Danielle Cardoso Geraldo [UNESP] Urbaczek, Ana Carolina [UNESP] Carlos, Iracilda Zeppone [UNESP] Vellosa, José Carlos Rebuglio |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Manente, Francine Alessandra [UNESP] da Silva, Ana Flavia Tostes [UNESP] Schemberger, Josiane Aparecida [UNESP] Heinrich, Isabella Aparecida [UNESP] Munhoz, Antonio Carlos Mattar [UNESP] Beltrame, Flávio Luis [UNESP] Maia, Danielle Cardoso Geraldo [UNESP] Urbaczek, Ana Carolina [UNESP] Carlos, Iracilda Zeppone [UNESP] Vellosa, José Carlos Rebuglio |
dc.subject.por.fl_str_mv |
Amphotericin B Cytotoxicity Hesperidin Oxidative stress |
topic |
Amphotericin B Cytotoxicity Hesperidin Oxidative stress |
description |
Amphotericin B (AmB) is drug “gold standard” for the treatment of invasive fungal infections since 1960. However, amphotericin B has high toxicity, which manifests itself most often in the kidneys and in the liver. It is known, since 1985, that self-oxidation of AmB gives different forms of reactive oxidative species and these, being toxic to the cell, would be responsible, in part, by its toxicity. Different studies indicate that hesperidin contributes, through the reduction of oxidative stress, to protect against renal injury generated by ischemia. This fact and the involvement of AmB in the generation of free radicals make it interesting the preliminary evaluation of the effect of hesperidin and AmB (alone or associated) against reactive oxygen species and free radicals, as well as the study on models of cytotoxicity. Front ABTS•+, AmB presented IC<inf>50</inf> equal to 0.0124 mg/mL, but when it was associated to hesperidin this value has decreased to 0.0003 mg/mL. Front HOCl, Amb presented IC<inf>50</inf> equal to 0.0056, but when it was associated to hesperidin this value has decreased to 0.0023 mg/mL. In the trials with DPPH• and the superoxide anion radical samples were not effective. In the assay with endotelial cell culture (HUVEC cells), the association has decreased cell viability. These preliminary results demonstrate the interaction of the compounds with reactive species as well as indicate the possibility of damage exacerbation by AmB in the presence of antioxidants in an in vitro model. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2022-04-29T07:26:24Z 2022-04-29T07:26:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 35, n. 4, p. 679-685, 2014. 1808-4532 http://hdl.handle.net/11449/228031 2-s2.0-84939503542 |
identifier_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 35, n. 4, p. 679-685, 2014. 1808-4532 2-s2.0-84939503542 |
url |
http://hdl.handle.net/11449/228031 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
679-685 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792961489754128384 |