Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.yhbeh.2016.01.002 http://hdl.handle.net/11449/164713 |
Resumo: | It has been shown that the bed nucleus of the stria terminalis (BNST) of rats contains nitrergic neurons, which are activated during animal exposure to aversive stimuli. The BNST is also populated by glutamatergic and corticotrophin releasing factor (CRFergic) neurons, which in turn are activated under stressful situations. Here we investigated the anxiogenic-like effects of intra-BNST injections of a nitric oxide (NO) donor, NOC-9 in mice. The role of CRFergic and glutamatergic systems on defensive behavior induced by NOC-9 was investigated with previous intra-BNST infusion of different doses of CP376395, a CRF type 1 receptor antagonist (CRF1), or AP-7, an NMDA (N-methyl-D-aspartate) receptor antagonist. Anxiety-like behavior was assessed immediately and 5 min after intra-BNST drug injection, exposing mice to a novel arena and to the elevated plus-maze (EPM; an anxiogenic situation). Results showed that NOC-9 provoked a short period (approximate to 150 s) of freezing behavior in the novel arena and increased anxiety in the EPM. Both CP and AP-7 attenuated the anxiogenic-like effects of NOC-9 in the EPM without changing freezing behavior in the novel arena. When given alone (i.e. without prior intra-BNST injection of NOC-9), AP-7 (0.20 nmol), but not CP (0.75, 1.50, or 3.00 nmol), attenuated anxiety in mice exposed to the EPM. These results suggest that CRF1 and NMDA receptors located within the BNST differentially modulate aversive effects induced by NO production in this limbic forebrain structure. (C) 2016 Elsevier Inc. All rights reserved. |
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Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptorsBed nucleus of the stria terminalisNitric oxideFreezingAnxietyCRF1 and NMDA receptorsMiceIt has been shown that the bed nucleus of the stria terminalis (BNST) of rats contains nitrergic neurons, which are activated during animal exposure to aversive stimuli. The BNST is also populated by glutamatergic and corticotrophin releasing factor (CRFergic) neurons, which in turn are activated under stressful situations. Here we investigated the anxiogenic-like effects of intra-BNST injections of a nitric oxide (NO) donor, NOC-9 in mice. The role of CRFergic and glutamatergic systems on defensive behavior induced by NOC-9 was investigated with previous intra-BNST infusion of different doses of CP376395, a CRF type 1 receptor antagonist (CRF1), or AP-7, an NMDA (N-methyl-D-aspartate) receptor antagonist. Anxiety-like behavior was assessed immediately and 5 min after intra-BNST drug injection, exposing mice to a novel arena and to the elevated plus-maze (EPM; an anxiogenic situation). Results showed that NOC-9 provoked a short period (approximate to 150 s) of freezing behavior in the novel arena and increased anxiety in the EPM. Both CP and AP-7 attenuated the anxiogenic-like effects of NOC-9 in the EPM without changing freezing behavior in the novel arena. When given alone (i.e. without prior intra-BNST injection of NOC-9), AP-7 (0.20 nmol), but not CP (0.75, 1.50, or 3.00 nmol), attenuated anxiety in mice exposed to the EPM. These results suggest that CRF1 and NMDA receptors located within the BNST differentially modulate aversive effects induced by NO production in this limbic forebrain structure. (C) 2016 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Carlos, Programa Interinst Posgrad Ciencias Fisiol, Araraquara, SP, BrazilUniv Estadual Paulista, BR-14800903 Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Farmacol Lab, BR-14800903 Araraquara, SP, BrazilUniv Fed Uberlandia, Farmacol, Av Engenheiro Dinz 1178,CP 593, BR-38400 Uberlandia, MG, BrazilUniv Estadual Paulista, BR-14800903 Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Farmacol Lab, BR-14800903 Araraquara, SP, BrazilFAPESP: 2013/01383-6CNPq: 478696/2013-2CNPq: 305597/2012-4Elsevier B.V.Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)Faria, M. P. [UNESP]Miguel, T. T.Gomes, K. S. [UNESP]Nunes-de-Souza, Rl. [UNESP]2018-11-26T17:55:47Z2018-11-26T17:55:47Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article74-83application/pdfhttp://dx.doi.org/10.1016/j.yhbeh.2016.01.002Hormones And Behavior. San Diego: Academic Press Inc Elsevier Science, v. 79, p. 74-83, 2016.0018-506Xhttp://hdl.handle.net/11449/16471310.1016/j.yhbeh.2016.01.002WOS:000370468000010WOS000370468000010.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHormones And Behaviorinfo:eu-repo/semantics/openAccess2023-11-27T06:11:52Zoai:repositorio.unesp.br:11449/164713Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-27T06:11:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
title |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
spellingShingle |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors Faria, M. P. [UNESP] Bed nucleus of the stria terminalis Nitric oxide Freezing Anxiety CRF1 and NMDA receptors Mice |
title_short |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
title_full |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
title_fullStr |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
title_full_unstemmed |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
title_sort |
Anxiety-like responses induced by nitric oxide within the BNST in mice: Role of CRF1 and NMDA receptors |
author |
Faria, M. P. [UNESP] |
author_facet |
Faria, M. P. [UNESP] Miguel, T. T. Gomes, K. S. [UNESP] Nunes-de-Souza, Rl. [UNESP] |
author_role |
author |
author2 |
Miguel, T. T. Gomes, K. S. [UNESP] Nunes-de-Souza, Rl. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Faria, M. P. [UNESP] Miguel, T. T. Gomes, K. S. [UNESP] Nunes-de-Souza, Rl. [UNESP] |
dc.subject.por.fl_str_mv |
Bed nucleus of the stria terminalis Nitric oxide Freezing Anxiety CRF1 and NMDA receptors Mice |
topic |
Bed nucleus of the stria terminalis Nitric oxide Freezing Anxiety CRF1 and NMDA receptors Mice |
description |
It has been shown that the bed nucleus of the stria terminalis (BNST) of rats contains nitrergic neurons, which are activated during animal exposure to aversive stimuli. The BNST is also populated by glutamatergic and corticotrophin releasing factor (CRFergic) neurons, which in turn are activated under stressful situations. Here we investigated the anxiogenic-like effects of intra-BNST injections of a nitric oxide (NO) donor, NOC-9 in mice. The role of CRFergic and glutamatergic systems on defensive behavior induced by NOC-9 was investigated with previous intra-BNST infusion of different doses of CP376395, a CRF type 1 receptor antagonist (CRF1), or AP-7, an NMDA (N-methyl-D-aspartate) receptor antagonist. Anxiety-like behavior was assessed immediately and 5 min after intra-BNST drug injection, exposing mice to a novel arena and to the elevated plus-maze (EPM; an anxiogenic situation). Results showed that NOC-9 provoked a short period (approximate to 150 s) of freezing behavior in the novel arena and increased anxiety in the EPM. Both CP and AP-7 attenuated the anxiogenic-like effects of NOC-9 in the EPM without changing freezing behavior in the novel arena. When given alone (i.e. without prior intra-BNST injection of NOC-9), AP-7 (0.20 nmol), but not CP (0.75, 1.50, or 3.00 nmol), attenuated anxiety in mice exposed to the EPM. These results suggest that CRF1 and NMDA receptors located within the BNST differentially modulate aversive effects induced by NO production in this limbic forebrain structure. (C) 2016 Elsevier Inc. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 2018-11-26T17:55:47Z 2018-11-26T17:55:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.yhbeh.2016.01.002 Hormones And Behavior. San Diego: Academic Press Inc Elsevier Science, v. 79, p. 74-83, 2016. 0018-506X http://hdl.handle.net/11449/164713 10.1016/j.yhbeh.2016.01.002 WOS:000370468000010 WOS000370468000010.pdf |
url |
http://dx.doi.org/10.1016/j.yhbeh.2016.01.002 http://hdl.handle.net/11449/164713 |
identifier_str_mv |
Hormones And Behavior. San Diego: Academic Press Inc Elsevier Science, v. 79, p. 74-83, 2016. 0018-506X 10.1016/j.yhbeh.2016.01.002 WOS:000370468000010 WOS000370468000010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hormones And Behavior |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
74-83 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965085506994176 |