Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/mmy/myx098 http://hdl.handle.net/11449/166309 |
Resumo: | This report describes a model of host resistance for Sporothrix schenckii, an opportunistic fungi in immunosuppressed mice with cyclophosphamide (CY) to be used in studies of immunotoxicology and immunopharmacology. Two doses of CY were administered intraperitoneally: 200 mg/kg and a booster of 150 mg/kg at 9-day intervals. Three days after the first dose of CY the animals were infected subcutaneously with 1.8 x 10(8) yeast/ml (S. schenckii ATCC 16345). At 7 and 14 days post-infection, the animals were euthanized and analyzed the fungal load by unit forming colony count in the spleen and popliteal lymph nodes. The relative weight of thymus and spleen, splenic index, the frequency of T and B cells in spleen by flow cytometry, the hind paw inflammation index and cytokine (interleukin [IL]-17, IL-10, and interferon [IFN]-gamma) profile were measured. Histopathological studies of the spleen and the hind paw were also assessed. The immunosuppression status was confirmed at the evaluated days by reduction of relative weight of thymus, reduction of the splenic white pulp, the population of B and T lymphocytes, and the cytokine profile in the treated mice with CY in comparison with nontreated groups, associated to higher fungal load in hind paw and spleen in the infected mice. The described model reveals an increasing in susceptibility to infection and severity when associated with immunosuppression. This model can serve as a reference for studies of S. schenckii host resistance in pharmaceutical and toxicological studies. |
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Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice modelSporothrix schenckiihost resistance modelsimmunosuppressioncyclophosphamideThis report describes a model of host resistance for Sporothrix schenckii, an opportunistic fungi in immunosuppressed mice with cyclophosphamide (CY) to be used in studies of immunotoxicology and immunopharmacology. Two doses of CY were administered intraperitoneally: 200 mg/kg and a booster of 150 mg/kg at 9-day intervals. Three days after the first dose of CY the animals were infected subcutaneously with 1.8 x 10(8) yeast/ml (S. schenckii ATCC 16345). At 7 and 14 days post-infection, the animals were euthanized and analyzed the fungal load by unit forming colony count in the spleen and popliteal lymph nodes. The relative weight of thymus and spleen, splenic index, the frequency of T and B cells in spleen by flow cytometry, the hind paw inflammation index and cytokine (interleukin [IL]-17, IL-10, and interferon [IFN]-gamma) profile were measured. Histopathological studies of the spleen and the hind paw were also assessed. The immunosuppression status was confirmed at the evaluated days by reduction of relative weight of thymus, reduction of the splenic white pulp, the population of B and T lymphocytes, and the cytokine profile in the treated mice with CY in comparison with nontreated groups, associated to higher fungal load in hind paw and spleen in the infected mice. The described model reveals an increasing in susceptibility to infection and severity when associated with immunosuppression. This model can serve as a reference for studies of S. schenckii host resistance in pharmaceutical and toxicological studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Araraquara, SP, BrazilSao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Araraquara, SP, BrazilFAPESP: 2014/00914-5FAPESP: 2015/04023-0Oxford Univ PressUniversidade Estadual Paulista (Unesp)Manente, Francine Alessandra [UNESP]Quinello, Camila [UNESP]Ferreira, Lucas Souza [UNESP]Andrade, Cleverton Roberto de [UNESP]Jellmayer, Juliana Aparecida [UNESP]Portuondo, Deivys Leandro [UNESP]Batista-Duharte, Alexander [UNESP]Carlos, Iracilda Zeppone [UNESP]2018-11-30T03:29:14Z2018-11-30T03:29:14Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article711-722http://dx.doi.org/10.1093/mmy/myx098Medical Mycology. Oxford: Oxford Univ Press, v. 56, n. 6, p. 711-722, 2018.1369-3786http://hdl.handle.net/11449/16630910.1093/mmy/myx098WOS:00044434720000724026829697768750000-0001-7015-7175Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Mycologyinfo:eu-repo/semantics/openAccess2022-02-11T18:14:35Zoai:repositorio.unesp.br:11449/166309Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-02-11T18:14:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
title |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
spellingShingle |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model Manente, Francine Alessandra [UNESP] Sporothrix schenckii host resistance models immunosuppression cyclophosphamide |
title_short |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
title_full |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
title_fullStr |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
title_full_unstemmed |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
title_sort |
Experimental sporotrichosis in a cyclophosphamide-induced immunosuppressed mice model |
author |
Manente, Francine Alessandra [UNESP] |
author_facet |
Manente, Francine Alessandra [UNESP] Quinello, Camila [UNESP] Ferreira, Lucas Souza [UNESP] Andrade, Cleverton Roberto de [UNESP] Jellmayer, Juliana Aparecida [UNESP] Portuondo, Deivys Leandro [UNESP] Batista-Duharte, Alexander [UNESP] Carlos, Iracilda Zeppone [UNESP] |
author_role |
author |
author2 |
Quinello, Camila [UNESP] Ferreira, Lucas Souza [UNESP] Andrade, Cleverton Roberto de [UNESP] Jellmayer, Juliana Aparecida [UNESP] Portuondo, Deivys Leandro [UNESP] Batista-Duharte, Alexander [UNESP] Carlos, Iracilda Zeppone [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Manente, Francine Alessandra [UNESP] Quinello, Camila [UNESP] Ferreira, Lucas Souza [UNESP] Andrade, Cleverton Roberto de [UNESP] Jellmayer, Juliana Aparecida [UNESP] Portuondo, Deivys Leandro [UNESP] Batista-Duharte, Alexander [UNESP] Carlos, Iracilda Zeppone [UNESP] |
dc.subject.por.fl_str_mv |
Sporothrix schenckii host resistance models immunosuppression cyclophosphamide |
topic |
Sporothrix schenckii host resistance models immunosuppression cyclophosphamide |
description |
This report describes a model of host resistance for Sporothrix schenckii, an opportunistic fungi in immunosuppressed mice with cyclophosphamide (CY) to be used in studies of immunotoxicology and immunopharmacology. Two doses of CY were administered intraperitoneally: 200 mg/kg and a booster of 150 mg/kg at 9-day intervals. Three days after the first dose of CY the animals were infected subcutaneously with 1.8 x 10(8) yeast/ml (S. schenckii ATCC 16345). At 7 and 14 days post-infection, the animals were euthanized and analyzed the fungal load by unit forming colony count in the spleen and popliteal lymph nodes. The relative weight of thymus and spleen, splenic index, the frequency of T and B cells in spleen by flow cytometry, the hind paw inflammation index and cytokine (interleukin [IL]-17, IL-10, and interferon [IFN]-gamma) profile were measured. Histopathological studies of the spleen and the hind paw were also assessed. The immunosuppression status was confirmed at the evaluated days by reduction of relative weight of thymus, reduction of the splenic white pulp, the population of B and T lymphocytes, and the cytokine profile in the treated mice with CY in comparison with nontreated groups, associated to higher fungal load in hind paw and spleen in the infected mice. The described model reveals an increasing in susceptibility to infection and severity when associated with immunosuppression. This model can serve as a reference for studies of S. schenckii host resistance in pharmaceutical and toxicological studies. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-30T03:29:14Z 2018-11-30T03:29:14Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/mmy/myx098 Medical Mycology. Oxford: Oxford Univ Press, v. 56, n. 6, p. 711-722, 2018. 1369-3786 http://hdl.handle.net/11449/166309 10.1093/mmy/myx098 WOS:000444347200007 2402682969776875 0000-0001-7015-7175 |
url |
http://dx.doi.org/10.1093/mmy/myx098 http://hdl.handle.net/11449/166309 |
identifier_str_mv |
Medical Mycology. Oxford: Oxford Univ Press, v. 56, n. 6, p. 711-722, 2018. 1369-3786 10.1093/mmy/myx098 WOS:000444347200007 2402682969776875 0000-0001-7015-7175 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
711-722 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961477075795968 |