Satellite dna in neotropical deer species
Main Author: | |
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Publication Date: | 2021 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3390/genes12010123 http://hdl.handle.net/11449/205763 |
Summary: | The taxonomy and phylogenetics of Neotropical deer have been mostly based on morpho-logical criteria and needs a critical revision on the basis of new molecular and cytogenetic markers. In this study, we used the variation in the sequence, copy number, and chromosome localization of satellite I-IV DNA to evaluate evolutionary relationships among eight Neotropical deer species. Using FISH with satI-IV probes derived from Mazama gouazoubira, we proved the presence of satellite DNA blocks in peri/centromeric regions of all analyzed deer. Satellite DNA was also detected in the interstitial chromosome regions of species of the genus Mazama with highly reduced chromosome numbers. In contrast to Blastocerus dichotomus, Ozotoceros bezoarticus, and Odocoileus virginianus, Mazama species showed high abundance of satIV DNA by FISH. The phylogenetic analysis of the satellite DNA showed close relationships between O. bezoarticus and B. dichotomus. Furthermore, the Neotropical and Nearctic populations of O. virginianus formed a single clade. However, the satellite DNA phylogeny did not allow resolving the relationships within the genus Mazama. The high abundance of the satellite DNA in centromeres probably contributes to the formation of chromosomal rearrangements, thus leading to a fast and ongoing speciation in this genus, which has not yet been reflected in the satellite DNA sequence diversification. |
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Satellite dna in neotropical deer speciesCervidaeComparative cytogeneticsFISHSatellite DNASequencingThe taxonomy and phylogenetics of Neotropical deer have been mostly based on morpho-logical criteria and needs a critical revision on the basis of new molecular and cytogenetic markers. In this study, we used the variation in the sequence, copy number, and chromosome localization of satellite I-IV DNA to evaluate evolutionary relationships among eight Neotropical deer species. Using FISH with satI-IV probes derived from Mazama gouazoubira, we proved the presence of satellite DNA blocks in peri/centromeric regions of all analyzed deer. Satellite DNA was also detected in the interstitial chromosome regions of species of the genus Mazama with highly reduced chromosome numbers. In contrast to Blastocerus dichotomus, Ozotoceros bezoarticus, and Odocoileus virginianus, Mazama species showed high abundance of satIV DNA by FISH. The phylogenetic analysis of the satellite DNA showed close relationships between O. bezoarticus and B. dichotomus. Furthermore, the Neotropical and Nearctic populations of O. virginianus formed a single clade. However, the satellite DNA phylogeny did not allow resolving the relationships within the genus Mazama. The high abundance of the satellite DNA in centromeres probably contributes to the formation of chromosomal rearrangements, thus leading to a fast and ongoing speciation in this genus, which has not yet been reflected in the satellite DNA sequence diversification.Fondo Nacional de Desarrollo Científico y TecnológicoGrantová Agentura České RepublikyFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Ministerstvo Školství, Mládeže a TělovýchovyDepartment of Genetics and Reproductive Biotechnologies Central European Institute of Technology—Veterinary Research Institute, Hudcova 70Institute of Vertebrate Biology Czech Academy of Sciences, Kvetna 8Deer Research and Conservation Center (NUPECCE) School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Deer Research and Conservation Center (NUPECCE) School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Fondo Nacional de Desarrollo Científico y Tecnológico: 116-2017Grantová Agentura České Republiky: 20-22517JFAPESP: 2019/06940-1-GACRMinisterstvo Školství, Mládeže a Tělovýchovy: LQ1601Central European Institute of Technology—Veterinary Research InstituteCzech Academy of SciencesUniversidade Estadual Paulista (Unesp)Vozdova, MiluseKubickova, SvatavaMartínková, NatáliaGalindo, David Javier [UNESP]Bernegossi, Agda Maria [UNESP]Cernohorska, HalinaKadlcikova, DitaMusilová, PetraDuarte, Jose Mauricio [UNESP]Rubes, Jiri2021-06-25T10:20:49Z2021-06-25T10:20:49Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-17http://dx.doi.org/10.3390/genes12010123Genes, v. 12, n. 1, p. 1-17, 2021.2073-4425http://hdl.handle.net/11449/20576310.3390/genes120101232-s2.0-85099646701Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2021-10-22T17:11:17Zoai:repositorio.unesp.br:11449/205763Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T17:11:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Satellite dna in neotropical deer species |
title |
Satellite dna in neotropical deer species |
spellingShingle |
Satellite dna in neotropical deer species Vozdova, Miluse Cervidae Comparative cytogenetics FISH Satellite DNA Sequencing |
title_short |
Satellite dna in neotropical deer species |
title_full |
Satellite dna in neotropical deer species |
title_fullStr |
Satellite dna in neotropical deer species |
title_full_unstemmed |
Satellite dna in neotropical deer species |
title_sort |
Satellite dna in neotropical deer species |
author |
Vozdova, Miluse |
author_facet |
Vozdova, Miluse Kubickova, Svatava Martínková, Natália Galindo, David Javier [UNESP] Bernegossi, Agda Maria [UNESP] Cernohorska, Halina Kadlcikova, Dita Musilová, Petra Duarte, Jose Mauricio [UNESP] Rubes, Jiri |
author_role |
author |
author2 |
Kubickova, Svatava Martínková, Natália Galindo, David Javier [UNESP] Bernegossi, Agda Maria [UNESP] Cernohorska, Halina Kadlcikova, Dita Musilová, Petra Duarte, Jose Mauricio [UNESP] Rubes, Jiri |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Central European Institute of Technology—Veterinary Research Institute Czech Academy of Sciences Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vozdova, Miluse Kubickova, Svatava Martínková, Natália Galindo, David Javier [UNESP] Bernegossi, Agda Maria [UNESP] Cernohorska, Halina Kadlcikova, Dita Musilová, Petra Duarte, Jose Mauricio [UNESP] Rubes, Jiri |
dc.subject.por.fl_str_mv |
Cervidae Comparative cytogenetics FISH Satellite DNA Sequencing |
topic |
Cervidae Comparative cytogenetics FISH Satellite DNA Sequencing |
description |
The taxonomy and phylogenetics of Neotropical deer have been mostly based on morpho-logical criteria and needs a critical revision on the basis of new molecular and cytogenetic markers. In this study, we used the variation in the sequence, copy number, and chromosome localization of satellite I-IV DNA to evaluate evolutionary relationships among eight Neotropical deer species. Using FISH with satI-IV probes derived from Mazama gouazoubira, we proved the presence of satellite DNA blocks in peri/centromeric regions of all analyzed deer. Satellite DNA was also detected in the interstitial chromosome regions of species of the genus Mazama with highly reduced chromosome numbers. In contrast to Blastocerus dichotomus, Ozotoceros bezoarticus, and Odocoileus virginianus, Mazama species showed high abundance of satIV DNA by FISH. The phylogenetic analysis of the satellite DNA showed close relationships between O. bezoarticus and B. dichotomus. Furthermore, the Neotropical and Nearctic populations of O. virginianus formed a single clade. However, the satellite DNA phylogeny did not allow resolving the relationships within the genus Mazama. The high abundance of the satellite DNA in centromeres probably contributes to the formation of chromosomal rearrangements, thus leading to a fast and ongoing speciation in this genus, which has not yet been reflected in the satellite DNA sequence diversification. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:20:49Z 2021-06-25T10:20:49Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/genes12010123 Genes, v. 12, n. 1, p. 1-17, 2021. 2073-4425 http://hdl.handle.net/11449/205763 10.3390/genes12010123 2-s2.0-85099646701 |
url |
http://dx.doi.org/10.3390/genes12010123 http://hdl.handle.net/11449/205763 |
identifier_str_mv |
Genes, v. 12, n. 1, p. 1-17, 2021. 2073-4425 10.3390/genes12010123 2-s2.0-85099646701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-17 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964496219865088 |