Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes

Detalhes bibliográficos
Autor(a) principal: Garbellini, Gustavo S. [UNESP]
Data de Publicação: 2011
Outros Autores: Uliana, Carolina V. [UNESP], Yamanaka, Hideko [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0103-50532011000700007
http://hdl.handle.net/11449/25853
Resumo: The individual detection and equimolar mixture of DNA nucleotides guanosine monophosphate (GMP), adenosine monophosphate (AMP), thymidine (TMP) and cytidine (CMP) 5'-monophosphate using square wave voltammetry was performed on boron doped diamond (BDD) electrodes cathodically (Red-DDB) and anodically (Oxi-DDB) pretreated. The oxidation of individual DNA nucleotides was more sensitive on Oxi-BDD electrode. In a simultaneous detection of nucleotides, the responses of GMP, AMP, TMP and CMP were very adequate on both treated electrodes. Particularly, more sensitive and separate peaks for TMP and CMP on Oxi-BDD and Red-BDD electrodes, respectively, were observed after deconvolution procedure. The detection of nucleotides in aqueous solutions will certainly contribute for genotoxic evaluation of substances and hybridization reactions by immobilizing ss or ds-DNA on BDD surface.
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spelling Detection of DNA Nucleotides on Pretreated Boron Doped Diamond ElectrodesDNAnucleotidesdiamond electrodessurface terminationThe individual detection and equimolar mixture of DNA nucleotides guanosine monophosphate (GMP), adenosine monophosphate (AMP), thymidine (TMP) and cytidine (CMP) 5'-monophosphate using square wave voltammetry was performed on boron doped diamond (BDD) electrodes cathodically (Red-DDB) and anodically (Oxi-DDB) pretreated. The oxidation of individual DNA nucleotides was more sensitive on Oxi-BDD electrode. In a simultaneous detection of nucleotides, the responses of GMP, AMP, TMP and CMP were very adequate on both treated electrodes. Particularly, more sensitive and separate peaks for TMP and CMP on Oxi-BDD and Red-BDD electrodes, respectively, were observed after deconvolution procedure. The detection of nucleotides in aqueous solutions will certainly contribute for genotoxic evaluation of substances and hybridization reactions by immobilizing ss or ds-DNA on BDD surface.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP, BrazilSão Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP, BrazilFAPESP: 09/08161-8FAPESP: 08/08990-1Soc Brasileira QuimicaUniversidade Estadual Paulista (Unesp)Garbellini, Gustavo S. [UNESP]Uliana, Carolina V. [UNESP]Yamanaka, Hideko [UNESP]2014-05-20T14:19:24Z2014-05-20T14:19:24Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1241-1245http://dx.doi.org/10.1590/S0103-50532011000700007Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 22, n. 7, p. 1241-1245, 2011.0103-5053http://hdl.handle.net/11449/2585310.1590/S0103-50532011000700007S0103-50532011000700007WOS:000293388500007S0103-50532011000700007.pdf1923726000036625Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Brazilian Chemical Society1.4440,357info:eu-repo/semantics/openAccess2021-10-23T17:45:47Zoai:repositorio.unesp.br:11449/25853Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:45:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
title Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
spellingShingle Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
Garbellini, Gustavo S. [UNESP]
DNA
nucleotides
diamond electrodes
surface termination
title_short Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
title_full Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
title_fullStr Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
title_full_unstemmed Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
title_sort Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
author Garbellini, Gustavo S. [UNESP]
author_facet Garbellini, Gustavo S. [UNESP]
Uliana, Carolina V. [UNESP]
Yamanaka, Hideko [UNESP]
author_role author
author2 Uliana, Carolina V. [UNESP]
Yamanaka, Hideko [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Garbellini, Gustavo S. [UNESP]
Uliana, Carolina V. [UNESP]
Yamanaka, Hideko [UNESP]
dc.subject.por.fl_str_mv DNA
nucleotides
diamond electrodes
surface termination
topic DNA
nucleotides
diamond electrodes
surface termination
description The individual detection and equimolar mixture of DNA nucleotides guanosine monophosphate (GMP), adenosine monophosphate (AMP), thymidine (TMP) and cytidine (CMP) 5'-monophosphate using square wave voltammetry was performed on boron doped diamond (BDD) electrodes cathodically (Red-DDB) and anodically (Oxi-DDB) pretreated. The oxidation of individual DNA nucleotides was more sensitive on Oxi-BDD electrode. In a simultaneous detection of nucleotides, the responses of GMP, AMP, TMP and CMP were very adequate on both treated electrodes. Particularly, more sensitive and separate peaks for TMP and CMP on Oxi-BDD and Red-BDD electrodes, respectively, were observed after deconvolution procedure. The detection of nucleotides in aqueous solutions will certainly contribute for genotoxic evaluation of substances and hybridization reactions by immobilizing ss or ds-DNA on BDD surface.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2014-05-20T14:19:24Z
2014-05-20T14:19:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0103-50532011000700007
Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 22, n. 7, p. 1241-1245, 2011.
0103-5053
http://hdl.handle.net/11449/25853
10.1590/S0103-50532011000700007
S0103-50532011000700007
WOS:000293388500007
S0103-50532011000700007.pdf
1923726000036625
url http://dx.doi.org/10.1590/S0103-50532011000700007
http://hdl.handle.net/11449/25853
identifier_str_mv Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 22, n. 7, p. 1241-1245, 2011.
0103-5053
10.1590/S0103-50532011000700007
S0103-50532011000700007
WOS:000293388500007
S0103-50532011000700007.pdf
1923726000036625
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Brazilian Chemical Society
1.444
0,357
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1241-1245
dc.publisher.none.fl_str_mv Soc Brasileira Quimica
publisher.none.fl_str_mv Soc Brasileira Quimica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964992932413440

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