Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-020-61587-x http://hdl.handle.net/11449/201639 |
Resumo: | Carnosine is a dipeptide abundantly found in human skeletal muscle, cardiac muscle and neuronal cells having numerous properties that confers performance enhancing effects, as well as a wide-range of potential therapeutic applications. A reliable and valid method for tissue carnosine quantification is crucial for advancing the knowledge on biological processes involved with carnosine metabolism. In this regard, proton magnetic resonance spectroscopy (1H-MRS) has been used as a non-invasive alternative to quantify carnosine in human skeletal muscle. However, carnosine quantification by 1H-MRS has some potential limitations that warrant a thorough experimental examination of its validity. The present investigation examined the reliability, accuracy and sensitivity for the determination of muscle carnosine in humans using in vitro and in vivo experiments and comparing it to reference method for carnosine quantification (high-performance liquid chromatography – HPLC). We used in vitro 1H-MRS to verify signal linearity and possible noise sources. Carnosine was determined in the m. gastrocnemius by 1H-MRS and HPLC to compare signal quality and convergent validity. 1H-MRS showed adequate discriminant validity, but limited reliability and poor agreement with a reference method. Low signal amplitude, low signal-to-noise ratio, and voxel repositioning are major sources of error. |
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Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity AssessmentCarnosine is a dipeptide abundantly found in human skeletal muscle, cardiac muscle and neuronal cells having numerous properties that confers performance enhancing effects, as well as a wide-range of potential therapeutic applications. A reliable and valid method for tissue carnosine quantification is crucial for advancing the knowledge on biological processes involved with carnosine metabolism. In this regard, proton magnetic resonance spectroscopy (1H-MRS) has been used as a non-invasive alternative to quantify carnosine in human skeletal muscle. However, carnosine quantification by 1H-MRS has some potential limitations that warrant a thorough experimental examination of its validity. The present investigation examined the reliability, accuracy and sensitivity for the determination of muscle carnosine in humans using in vitro and in vivo experiments and comparing it to reference method for carnosine quantification (high-performance liquid chromatography – HPLC). We used in vitro 1H-MRS to verify signal linearity and possible noise sources. Carnosine was determined in the m. gastrocnemius by 1H-MRS and HPLC to compare signal quality and convergent validity. 1H-MRS showed adequate discriminant validity, but limited reliability and poor agreement with a reference method. Low signal amplitude, low signal-to-noise ratio, and voxel repositioning are major sources of error.Applied Physiology and Nutrition Research Group School of Physical Education and Sport; Rheumatology Division; Faculdade de Medicina FMUSP Universidade de Sao PauloRheumatology Division; Faculdade de Medicina FMUSP Universidade de Sao PauloDepartment of Biochemistry and Technological Chemistry Universidade Estadual Paulista (UNESP)Sport Health and Performance Enhancement Research Centre; Musculoskeletal Physiology Research Group; School of Science and Technology Nottingham Trent UniversityLIM44 - Institute of Radiology and Oncology School of Medicine University of Sao PauloDepartment of Biochemistry and Technological Chemistry Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Nottingham Trent Universityda Eira Silva, ViniciusPainelli, Vitor de SallesShinjo, Samuel KatsuyukiRibeiro Pereira, WagnerCilli, Eduardo Maffud [UNESP]Sale, CraigGualano, BrunoOtaduy, Maria ConcepciónArtioli, Guilherme Giannini2020-12-12T02:37:56Z2020-12-12T02:37:56Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-020-61587-xScientific Reports, v. 10, n. 1, 2020.2045-2322http://hdl.handle.net/11449/20163910.1038/s41598-020-61587-x2-s2.0-85082020326Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-22T20:42:53Zoai:repositorio.unesp.br:11449/201639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:42:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
title |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
spellingShingle |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment da Eira Silva, Vinicius |
title_short |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
title_full |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
title_fullStr |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
title_full_unstemmed |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
title_sort |
Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment |
author |
da Eira Silva, Vinicius |
author_facet |
da Eira Silva, Vinicius Painelli, Vitor de Salles Shinjo, Samuel Katsuyuki Ribeiro Pereira, Wagner Cilli, Eduardo Maffud [UNESP] Sale, Craig Gualano, Bruno Otaduy, Maria Concepción Artioli, Guilherme Giannini |
author_role |
author |
author2 |
Painelli, Vitor de Salles Shinjo, Samuel Katsuyuki Ribeiro Pereira, Wagner Cilli, Eduardo Maffud [UNESP] Sale, Craig Gualano, Bruno Otaduy, Maria Concepción Artioli, Guilherme Giannini |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Nottingham Trent University |
dc.contributor.author.fl_str_mv |
da Eira Silva, Vinicius Painelli, Vitor de Salles Shinjo, Samuel Katsuyuki Ribeiro Pereira, Wagner Cilli, Eduardo Maffud [UNESP] Sale, Craig Gualano, Bruno Otaduy, Maria Concepción Artioli, Guilherme Giannini |
description |
Carnosine is a dipeptide abundantly found in human skeletal muscle, cardiac muscle and neuronal cells having numerous properties that confers performance enhancing effects, as well as a wide-range of potential therapeutic applications. A reliable and valid method for tissue carnosine quantification is crucial for advancing the knowledge on biological processes involved with carnosine metabolism. In this regard, proton magnetic resonance spectroscopy (1H-MRS) has been used as a non-invasive alternative to quantify carnosine in human skeletal muscle. However, carnosine quantification by 1H-MRS has some potential limitations that warrant a thorough experimental examination of its validity. The present investigation examined the reliability, accuracy and sensitivity for the determination of muscle carnosine in humans using in vitro and in vivo experiments and comparing it to reference method for carnosine quantification (high-performance liquid chromatography – HPLC). We used in vitro 1H-MRS to verify signal linearity and possible noise sources. Carnosine was determined in the m. gastrocnemius by 1H-MRS and HPLC to compare signal quality and convergent validity. 1H-MRS showed adequate discriminant validity, but limited reliability and poor agreement with a reference method. Low signal amplitude, low signal-to-noise ratio, and voxel repositioning are major sources of error. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:37:56Z 2020-12-12T02:37:56Z 2020-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-020-61587-x Scientific Reports, v. 10, n. 1, 2020. 2045-2322 http://hdl.handle.net/11449/201639 10.1038/s41598-020-61587-x 2-s2.0-85082020326 |
url |
http://dx.doi.org/10.1038/s41598-020-61587-x http://hdl.handle.net/11449/201639 |
identifier_str_mv |
Scientific Reports, v. 10, n. 1, 2020. 2045-2322 10.1038/s41598-020-61587-x 2-s2.0-85082020326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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