Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijbiomac.2016.11.123 http://hdl.handle.net/11449/178535 |
Resumo: | Exopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to be a bioemulsifier with potential applications in the degradation of hydrocarbons. In the present study, attempts were made to obtain the new exopolysaccharide production by Rhizobium tropici (SEMIA 4080 and MUTZC3) strains during growth on hydrocarbon substrate. Under the different cultivation conditions, the high molecular weight exopolysaccharides from Rhizobium tropici strains cultivated for 96 h mainly consisted of carbohydrates (79–85%) and a low percentage of protein. The EPSC3-D differed from the others, with only 60% of carbohydrate. However, all strains produced polymers with distinct rheology properties, such as viscosity of each EPS sample, suitable for different applications. In addition, RP-HPLC, FTIR and NMR studies revealed EPS produced by rhizobia strains were similar indicating minimal difference between EPS compositions. |
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Repositório Institucional da UNESP |
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Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrateBioemulsifierExopolysaccharidesRhizobium tropiciExopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to be a bioemulsifier with potential applications in the degradation of hydrocarbons. In the present study, attempts were made to obtain the new exopolysaccharide production by Rhizobium tropici (SEMIA 4080 and MUTZC3) strains during growth on hydrocarbon substrate. Under the different cultivation conditions, the high molecular weight exopolysaccharides from Rhizobium tropici strains cultivated for 96 h mainly consisted of carbohydrates (79–85%) and a low percentage of protein. The EPSC3-D differed from the others, with only 60% of carbohydrate. However, all strains produced polymers with distinct rheology properties, such as viscosity of each EPS sample, suitable for different applications. In addition, RP-HPLC, FTIR and NMR studies revealed EPS produced by rhizobia strains were similar indicating minimal difference between EPS compositions.São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalEmbrapa Instrumentação, Rua XV de Novembro 1452, São CarlosSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Castellane, Tereza Cristina Luque [UNESP]Campanharo, João Carlos [UNESP]Colnago, Luiz AlbertoCoutinho, Isabel DuarteLopes, Érica Mendes [UNESP]Lemos, Manoel Victor Franco [UNESP]de Macedo Lemos, Eliana Gertrudes [UNESP]2018-12-11T17:30:49Z2018-12-11T17:30:49Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article361-369application/pdfhttp://dx.doi.org/10.1016/j.ijbiomac.2016.11.123International Journal of Biological Macromolecules, v. 96, p. 361-369.1879-00030141-8130http://hdl.handle.net/11449/17853510.1016/j.ijbiomac.2016.11.1232-s2.0-850072372742-s2.0-85007237274.pdf7179273060624761Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Biological Macromolecules0,917info:eu-repo/semantics/openAccess2024-01-19T06:29:39Zoai:repositorio.unesp.br:11449/178535Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-19T06:29:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
title |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
spellingShingle |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate Castellane, Tereza Cristina Luque [UNESP] Bioemulsifier Exopolysaccharides Rhizobium tropici |
title_short |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
title_full |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
title_fullStr |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
title_full_unstemmed |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
title_sort |
Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate |
author |
Castellane, Tereza Cristina Luque [UNESP] |
author_facet |
Castellane, Tereza Cristina Luque [UNESP] Campanharo, João Carlos [UNESP] Colnago, Luiz Alberto Coutinho, Isabel Duarte Lopes, Érica Mendes [UNESP] Lemos, Manoel Victor Franco [UNESP] de Macedo Lemos, Eliana Gertrudes [UNESP] |
author_role |
author |
author2 |
Campanharo, João Carlos [UNESP] Colnago, Luiz Alberto Coutinho, Isabel Duarte Lopes, Érica Mendes [UNESP] Lemos, Manoel Victor Franco [UNESP] de Macedo Lemos, Eliana Gertrudes [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Castellane, Tereza Cristina Luque [UNESP] Campanharo, João Carlos [UNESP] Colnago, Luiz Alberto Coutinho, Isabel Duarte Lopes, Érica Mendes [UNESP] Lemos, Manoel Victor Franco [UNESP] de Macedo Lemos, Eliana Gertrudes [UNESP] |
dc.subject.por.fl_str_mv |
Bioemulsifier Exopolysaccharides Rhizobium tropici |
topic |
Bioemulsifier Exopolysaccharides Rhizobium tropici |
description |
Exopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to be a bioemulsifier with potential applications in the degradation of hydrocarbons. In the present study, attempts were made to obtain the new exopolysaccharide production by Rhizobium tropici (SEMIA 4080 and MUTZC3) strains during growth on hydrocarbon substrate. Under the different cultivation conditions, the high molecular weight exopolysaccharides from Rhizobium tropici strains cultivated for 96 h mainly consisted of carbohydrates (79–85%) and a low percentage of protein. The EPSC3-D differed from the others, with only 60% of carbohydrate. However, all strains produced polymers with distinct rheology properties, such as viscosity of each EPS sample, suitable for different applications. In addition, RP-HPLC, FTIR and NMR studies revealed EPS produced by rhizobia strains were similar indicating minimal difference between EPS compositions. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:30:49Z 2018-12-11T17:30:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijbiomac.2016.11.123 International Journal of Biological Macromolecules, v. 96, p. 361-369. 1879-0003 0141-8130 http://hdl.handle.net/11449/178535 10.1016/j.ijbiomac.2016.11.123 2-s2.0-85007237274 2-s2.0-85007237274.pdf 7179273060624761 |
url |
http://dx.doi.org/10.1016/j.ijbiomac.2016.11.123 http://hdl.handle.net/11449/178535 |
identifier_str_mv |
International Journal of Biological Macromolecules, v. 96, p. 361-369. 1879-0003 0141-8130 10.1016/j.ijbiomac.2016.11.123 2-s2.0-85007237274 2-s2.0-85007237274.pdf 7179273060624761 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Biological Macromolecules 0,917 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
361-369 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965663785123840 |