Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-018-36382-4 http://hdl.handle.net/11449/188530 |
Resumo: | This study reports the development of nanostructured hydrogels for the sustained release of the eutectic mixture of lidocaine and prilocaine (both at 2.5%) for intraoral topical use. The local anesthetics, free or encapsulated in poly(ε-caprolactone) nanocapsules, were incorporated into CARBOPOL hydrogel. The nanoparticle suspensions were characterized in vitro in terms of particle size, polydispersity, and surface charge, using dynamic light scattering measurements. The nanoparticle concentrations were determined by nanoparticle tracking analysis. Evaluation was made of physicochemical stability, structural features, encapsulation efficiency, and in vitro release kinetics. The CARBOPOL hydrogels were submitted to rheological, accelerated stability, and in vitro release tests, as well as determination of mechanical and mucoadhesive properties, in vitro cytotoxicity towards FGH and HaCaT cells, and in vitro permeation across buccal and palatal mucosa. Anesthetic efficacy was evaluated using Wistar rats. Nanocapsules were successfully developed that presented desirable physicochemical properties and a sustained release profile. The hydrogel formulations were stable for up to 6 months under critical conditions and exhibited non-Newtonian pseudoplastic flows, satisfactory mucoadhesive strength, non-cytotoxicity, and slow permeation across oral mucosa. In vivo assays revealed higher anesthetic efficacy in tail-flick tests, compared to a commercially available product. In conclusion, the proposed hydrogel has potential for provision of effective and longer-lasting superficial anesthesia at oral mucosa during medical and dental procedures. These results open perspectives for future clinical trials. |
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Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral AnesthesiaThis study reports the development of nanostructured hydrogels for the sustained release of the eutectic mixture of lidocaine and prilocaine (both at 2.5%) for intraoral topical use. The local anesthetics, free or encapsulated in poly(ε-caprolactone) nanocapsules, were incorporated into CARBOPOL hydrogel. The nanoparticle suspensions were characterized in vitro in terms of particle size, polydispersity, and surface charge, using dynamic light scattering measurements. The nanoparticle concentrations were determined by nanoparticle tracking analysis. Evaluation was made of physicochemical stability, structural features, encapsulation efficiency, and in vitro release kinetics. The CARBOPOL hydrogels were submitted to rheological, accelerated stability, and in vitro release tests, as well as determination of mechanical and mucoadhesive properties, in vitro cytotoxicity towards FGH and HaCaT cells, and in vitro permeation across buccal and palatal mucosa. Anesthetic efficacy was evaluated using Wistar rats. Nanocapsules were successfully developed that presented desirable physicochemical properties and a sustained release profile. The hydrogel formulations were stable for up to 6 months under critical conditions and exhibited non-Newtonian pseudoplastic flows, satisfactory mucoadhesive strength, non-cytotoxicity, and slow permeation across oral mucosa. In vivo assays revealed higher anesthetic efficacy in tail-flick tests, compared to a commercially available product. In conclusion, the proposed hydrogel has potential for provision of effective and longer-lasting superficial anesthesia at oral mucosa during medical and dental procedures. These results open perspectives for future clinical trials.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiological Sciences Piracicaba Dental School University of Campinas – UNICAMPSão Paulo State University – UNESP Institute of Science and Technology of Sorocaba Department of Environmental EngineeringDepartment of Biochemistry and Tissue Biology Institute of Biology University of Campinas – UNICAMPSão Paulo State University – UNESP Institute of Science and Technology of Sorocaba Department of Environmental EngineeringFAPESP: #2012/06974-4FAPESP: #2013/22326-5Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Muniz, Bruno VilelaBaratelli, Diego [UNESP]Di Carla, StephanySerpe, Lucianoda Silva, Camila BatistaGuilherme, Viviane AparecidaRibeiro, Lígia Nunes de MoraisCereda, Cintia Maria Saiade Paula, EneidaVolpato, Maria CristinaGroppo, Francisco CarlosFraceto, Leonardo Fernandes [UNESP]Franz-Montan, Michelle2019-10-06T16:11:11Z2019-10-06T16:11:11Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-018-36382-4Scientific Reports, v. 8, n. 1, 2018.2045-2322http://hdl.handle.net/11449/18853010.1038/s41598-018-36382-42-s2.0-85058876798Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T12:24:10Zoai:repositorio.unesp.br:11449/188530Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:24:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
title |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
spellingShingle |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia Muniz, Bruno Vilela |
title_short |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
title_full |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
title_fullStr |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
title_full_unstemmed |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
title_sort |
Hybrid Hydrogel Composed of Polymeric Nanocapsules Co-Loading Lidocaine and Prilocaine for Topical Intraoral Anesthesia |
author |
Muniz, Bruno Vilela |
author_facet |
Muniz, Bruno Vilela Baratelli, Diego [UNESP] Di Carla, Stephany Serpe, Luciano da Silva, Camila Batista Guilherme, Viviane Aparecida Ribeiro, Lígia Nunes de Morais Cereda, Cintia Maria Saia de Paula, Eneida Volpato, Maria Cristina Groppo, Francisco Carlos Fraceto, Leonardo Fernandes [UNESP] Franz-Montan, Michelle |
author_role |
author |
author2 |
Baratelli, Diego [UNESP] Di Carla, Stephany Serpe, Luciano da Silva, Camila Batista Guilherme, Viviane Aparecida Ribeiro, Lígia Nunes de Morais Cereda, Cintia Maria Saia de Paula, Eneida Volpato, Maria Cristina Groppo, Francisco Carlos Fraceto, Leonardo Fernandes [UNESP] Franz-Montan, Michelle |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Muniz, Bruno Vilela Baratelli, Diego [UNESP] Di Carla, Stephany Serpe, Luciano da Silva, Camila Batista Guilherme, Viviane Aparecida Ribeiro, Lígia Nunes de Morais Cereda, Cintia Maria Saia de Paula, Eneida Volpato, Maria Cristina Groppo, Francisco Carlos Fraceto, Leonardo Fernandes [UNESP] Franz-Montan, Michelle |
description |
This study reports the development of nanostructured hydrogels for the sustained release of the eutectic mixture of lidocaine and prilocaine (both at 2.5%) for intraoral topical use. The local anesthetics, free or encapsulated in poly(ε-caprolactone) nanocapsules, were incorporated into CARBOPOL hydrogel. The nanoparticle suspensions were characterized in vitro in terms of particle size, polydispersity, and surface charge, using dynamic light scattering measurements. The nanoparticle concentrations were determined by nanoparticle tracking analysis. Evaluation was made of physicochemical stability, structural features, encapsulation efficiency, and in vitro release kinetics. The CARBOPOL hydrogels were submitted to rheological, accelerated stability, and in vitro release tests, as well as determination of mechanical and mucoadhesive properties, in vitro cytotoxicity towards FGH and HaCaT cells, and in vitro permeation across buccal and palatal mucosa. Anesthetic efficacy was evaluated using Wistar rats. Nanocapsules were successfully developed that presented desirable physicochemical properties and a sustained release profile. The hydrogel formulations were stable for up to 6 months under critical conditions and exhibited non-Newtonian pseudoplastic flows, satisfactory mucoadhesive strength, non-cytotoxicity, and slow permeation across oral mucosa. In vivo assays revealed higher anesthetic efficacy in tail-flick tests, compared to a commercially available product. In conclusion, the proposed hydrogel has potential for provision of effective and longer-lasting superficial anesthesia at oral mucosa during medical and dental procedures. These results open perspectives for future clinical trials. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-06T16:11:11Z 2019-10-06T16:11:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-018-36382-4 Scientific Reports, v. 8, n. 1, 2018. 2045-2322 http://hdl.handle.net/11449/188530 10.1038/s41598-018-36382-4 2-s2.0-85058876798 |
url |
http://dx.doi.org/10.1038/s41598-018-36382-4 http://hdl.handle.net/11449/188530 |
identifier_str_mv |
Scientific Reports, v. 8, n. 1, 2018. 2045-2322 10.1038/s41598-018-36382-4 2-s2.0-85058876798 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790374006095872 |