Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbpa.2021.111025 http://hdl.handle.net/11449/229107 |
Resumo: | We investigated the impact of both the oral administration of hydrocortisone (HC) and an acute stressor on stress, innate immune responses and antioxidant system/oxidative stress responses of juvenile Piaractus mesopotamicus. Fish were either 1) given a commercial feed (C), 2) given a feed supplemented with 400 mg/kg HC, or 3) fed a commercial feed, chased for 2 min and exposed to air for 4 min (S). After initial sampling, fish C and HC were fed and sampled 1, 3, 6, 24 and 72 h post-feeding. Fish S were fed at the same time as the other groups, exposed to a stressor, and sampled 1, 3, 6, 24 and 72 h after. Exposure to the stressor increased circulating glucose and cortisol levels (at 1 and 3 h, respectively), while oral HC increased circulating cortisol at 1 h and glucose at 3 h. The stressor activated respiratory activity of leukocytes (RAL) at 3 h and reduced it at 6 h. HC did not activate RAL, but it did impair it at 6 h. The serum hemolytic activity of the complement system (HAC50) was impaired by the stressor at 1 and 3 h and by HC at 1 h. Regarding the antioxidant system, exposure to the stressor reduced glutathione peroxidase (GPx) and catalase (CAT) activity and decreased concentrations of reduced glutathione (GSH) in the liver up to 6 h. HC only impaired GPx. Additionally, stress induced the accumulation of melano-macrophage (MM) and melano-macrophage centers (MMC), which are biomarkers of oxidative stress, in the spleen. Differences in biomarkers in fish given cortisol and exposed to stress indicate that exogenous hormone was unable to precisely reproduce stress responses. |
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Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus)Innate immunityOxidative stressRedox balanceStressWe investigated the impact of both the oral administration of hydrocortisone (HC) and an acute stressor on stress, innate immune responses and antioxidant system/oxidative stress responses of juvenile Piaractus mesopotamicus. Fish were either 1) given a commercial feed (C), 2) given a feed supplemented with 400 mg/kg HC, or 3) fed a commercial feed, chased for 2 min and exposed to air for 4 min (S). After initial sampling, fish C and HC were fed and sampled 1, 3, 6, 24 and 72 h post-feeding. Fish S were fed at the same time as the other groups, exposed to a stressor, and sampled 1, 3, 6, 24 and 72 h after. Exposure to the stressor increased circulating glucose and cortisol levels (at 1 and 3 h, respectively), while oral HC increased circulating cortisol at 1 h and glucose at 3 h. The stressor activated respiratory activity of leukocytes (RAL) at 3 h and reduced it at 6 h. HC did not activate RAL, but it did impair it at 6 h. The serum hemolytic activity of the complement system (HAC50) was impaired by the stressor at 1 and 3 h and by HC at 1 h. Regarding the antioxidant system, exposure to the stressor reduced glutathione peroxidase (GPx) and catalase (CAT) activity and decreased concentrations of reduced glutathione (GSH) in the liver up to 6 h. HC only impaired GPx. Additionally, stress induced the accumulation of melano-macrophage (MM) and melano-macrophage centers (MMC), which are biomarkers of oxidative stress, in the spleen. Differences in biomarkers in fish given cortisol and exposed to stress indicate that exogenous hormone was unable to precisely reproduce stress responses.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista – UNESP Centro de Aquicultura da UNESP Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato CastellaneUniversidade Estadual Paulista – UNESP, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato CastellaneUniversidade Estadual Paulista – UNESP Faculdade de Ciências Agrárias e Tecnológicas, Rod. Cmte. João Ribeiro de Barros, Km 651Universidade Estadual Paulista – UNESP Centro de Aquicultura da UNESP Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato CastellaneUniversidade Estadual Paulista – UNESP, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato CastellaneUniversidade Estadual Paulista – UNESP Faculdade de Ciências Agrárias e Tecnológicas, Rod. Cmte. João Ribeiro de Barros, Km 651CNPq: 130581/2015-1Universidade Estadual Paulista (UNESP)Neyrão, Iuri Moraes [UNESP]Biller, Jaqueline Dalbello [UNESP]Takahashi, Leonardo Susumu [UNESP]Urbinati, Elisabeth Criscuolo [UNESP]2022-04-29T08:30:33Z2022-04-29T08:30:33Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cbpa.2021.111025Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, v. 260.1531-43321095-6433http://hdl.handle.net/11449/22910710.1016/j.cbpa.2021.1110252-s2.0-85109462040Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiologyinfo:eu-repo/semantics/openAccess2024-04-09T15:36:52Zoai:repositorio.unesp.br:11449/229107Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:36:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
title |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
spellingShingle |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) Neyrão, Iuri Moraes [UNESP] Innate immunity Oxidative stress Redox balance Stress |
title_short |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
title_full |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
title_fullStr |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
title_full_unstemmed |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
title_sort |
Modulation of immunity and hepatic antioxidant defense by corticosteroids in pacu (Piaractus mesopotamicus) |
author |
Neyrão, Iuri Moraes [UNESP] |
author_facet |
Neyrão, Iuri Moraes [UNESP] Biller, Jaqueline Dalbello [UNESP] Takahashi, Leonardo Susumu [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
author_role |
author |
author2 |
Biller, Jaqueline Dalbello [UNESP] Takahashi, Leonardo Susumu [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Neyrão, Iuri Moraes [UNESP] Biller, Jaqueline Dalbello [UNESP] Takahashi, Leonardo Susumu [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
dc.subject.por.fl_str_mv |
Innate immunity Oxidative stress Redox balance Stress |
topic |
Innate immunity Oxidative stress Redox balance Stress |
description |
We investigated the impact of both the oral administration of hydrocortisone (HC) and an acute stressor on stress, innate immune responses and antioxidant system/oxidative stress responses of juvenile Piaractus mesopotamicus. Fish were either 1) given a commercial feed (C), 2) given a feed supplemented with 400 mg/kg HC, or 3) fed a commercial feed, chased for 2 min and exposed to air for 4 min (S). After initial sampling, fish C and HC were fed and sampled 1, 3, 6, 24 and 72 h post-feeding. Fish S were fed at the same time as the other groups, exposed to a stressor, and sampled 1, 3, 6, 24 and 72 h after. Exposure to the stressor increased circulating glucose and cortisol levels (at 1 and 3 h, respectively), while oral HC increased circulating cortisol at 1 h and glucose at 3 h. The stressor activated respiratory activity of leukocytes (RAL) at 3 h and reduced it at 6 h. HC did not activate RAL, but it did impair it at 6 h. The serum hemolytic activity of the complement system (HAC50) was impaired by the stressor at 1 and 3 h and by HC at 1 h. Regarding the antioxidant system, exposure to the stressor reduced glutathione peroxidase (GPx) and catalase (CAT) activity and decreased concentrations of reduced glutathione (GSH) in the liver up to 6 h. HC only impaired GPx. Additionally, stress induced the accumulation of melano-macrophage (MM) and melano-macrophage centers (MMC), which are biomarkers of oxidative stress, in the spleen. Differences in biomarkers in fish given cortisol and exposed to stress indicate that exogenous hormone was unable to precisely reproduce stress responses. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-29T08:30:33Z 2022-04-29T08:30:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbpa.2021.111025 Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, v. 260. 1531-4332 1095-6433 http://hdl.handle.net/11449/229107 10.1016/j.cbpa.2021.111025 2-s2.0-85109462040 |
url |
http://dx.doi.org/10.1016/j.cbpa.2021.111025 http://hdl.handle.net/11449/229107 |
identifier_str_mv |
Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, v. 260. 1531-4332 1095-6433 10.1016/j.cbpa.2021.111025 2-s2.0-85109462040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789993046900736 |