Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/metabo11100650 http://hdl.handle.net/11449/233607 |
Resumo: | Oral squamous cell carcinoma (OSCC) represents 90% of oral malignant neoplasms. The search for specific biomarkers for OSCC is a very active field of research contributing to establishing early diagnostic methods and unraveling underlying pathogenic mechanisms. In this work we investigated the salivary metabolites and the metabolic pathways of OSCC aiming find possible biomarkers. Salivary metabolites samples from 27 OSCC patients and 41 control individuals were compared through a gas chromatography coupled to a mass spectrometer (GC-MS) technique. Our results allowed identification of pathways of the malate-aspartate shuttle, the beta-alanine metabolism, and the Warburg effect. The possible salivary biomarkers were identified using the area under receiver-operating curve (AUC) criterion. Twenty-four metabolites were identified with AUC > 0.8. Using the threshold of AUC = 0.9 we find malic acid, maltose, protocatechuic acid, lactose, 2-ketoadipic, and catechol metabolites expressed. We notice that this is the first report of salivary metabolome in South American oral cancer patients, to the best of our knowledge. Our findings regarding these metabolic changes are important in discovering salivary biomarkers of OSCC patients. However, additional work needs to be performed considering larger populations to validate our results. |
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Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinomaBiomarkersGC-MSMass spectrometryMetabolitesMetabolomicsOral cancerOral squamous cell carcinomaSalivaOral squamous cell carcinoma (OSCC) represents 90% of oral malignant neoplasms. The search for specific biomarkers for OSCC is a very active field of research contributing to establishing early diagnostic methods and unraveling underlying pathogenic mechanisms. In this work we investigated the salivary metabolites and the metabolic pathways of OSCC aiming find possible biomarkers. Salivary metabolites samples from 27 OSCC patients and 41 control individuals were compared through a gas chromatography coupled to a mass spectrometer (GC-MS) technique. Our results allowed identification of pathways of the malate-aspartate shuttle, the beta-alanine metabolism, and the Warburg effect. The possible salivary biomarkers were identified using the area under receiver-operating curve (AUC) criterion. Twenty-four metabolites were identified with AUC > 0.8. Using the threshold of AUC = 0.9 we find malic acid, maltose, protocatechuic acid, lactose, 2-ketoadipic, and catechol metabolites expressed. We notice that this is the first report of salivary metabolome in South American oral cancer patients, to the best of our knowledge. Our findings regarding these metabolic changes are important in discovering salivary biomarkers of OSCC patients. However, additional work needs to be performed considering larger populations to validate our results.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual PaulistaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biosciences and Diagnosis Institute of Science and Technology São Paulo State University (Unesp)Department of Head and Neck Surgery Hospital of the Pontifical Catholic University of CampinasCentro de Ciências Naturais e Humanas (CCNH) Universidade Federal do ABC, Av. dos Estados, 5001School of Dentistry Universidade Mogi das CruzesSchool of Medicine Anhembi Morumbi UniversityDempster MS Lab Department of Chemical Engineering Polytechnic School University of Sao PauloDepartment of Biosciences and Diagnosis Institute of Science and Technology São Paulo State University (Unesp)FAPESP: 2016/08633-0FAPESP: 2021/09168-8CNPq: 307718/2019-0Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Federal do ABC (UFABC)Universidade Mogi das CruzesAnhembi Morumbi UniversityUniversidade de São Paulo (USP)de Sá Alves, Mariana [UNESP]de Sá Rodrigues, Nayara [UNESP]Bandeira, Celso Muller [UNESP]Chagas, José Francisco SalesPascoal, Maria Beatriz NogueiraNepomuceno, Gabrielle Luana Jimenez Teodoroda Silva Martinho, HerculanoAlves, Mônica Ghislaine OliveiraMendes, Maria AnitaDias, MeriellenAlves, Levy Anderson CésarAlmeida, Janete Dias [UNESP]2022-05-01T09:30:57Z2022-05-01T09:30:57Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/metabo11100650Metabolites, v. 11, n. 10, 2021.2218-1989http://hdl.handle.net/11449/23360710.3390/metabo111006502-s2.0-85116077558Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMetabolitesinfo:eu-repo/semantics/openAccess2022-05-01T09:30:57Zoai:repositorio.unesp.br:11449/233607Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T09:30:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
title |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
spellingShingle |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma de Sá Alves, Mariana [UNESP] Biomarkers GC-MS Mass spectrometry Metabolites Metabolomics Oral cancer Oral squamous cell carcinoma Saliva |
title_short |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
title_full |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
title_fullStr |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
title_full_unstemmed |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
title_sort |
Identification of possible salivary metabolic biomarkers and altered metabolic pathways in south american patients diagnosed with oral squamous cell carcinoma |
author |
de Sá Alves, Mariana [UNESP] |
author_facet |
de Sá Alves, Mariana [UNESP] de Sá Rodrigues, Nayara [UNESP] Bandeira, Celso Muller [UNESP] Chagas, José Francisco Sales Pascoal, Maria Beatriz Nogueira Nepomuceno, Gabrielle Luana Jimenez Teodoro da Silva Martinho, Herculano Alves, Mônica Ghislaine Oliveira Mendes, Maria Anita Dias, Meriellen Alves, Levy Anderson César Almeida, Janete Dias [UNESP] |
author_role |
author |
author2 |
de Sá Rodrigues, Nayara [UNESP] Bandeira, Celso Muller [UNESP] Chagas, José Francisco Sales Pascoal, Maria Beatriz Nogueira Nepomuceno, Gabrielle Luana Jimenez Teodoro da Silva Martinho, Herculano Alves, Mônica Ghislaine Oliveira Mendes, Maria Anita Dias, Meriellen Alves, Levy Anderson César Almeida, Janete Dias [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) Universidade Federal do ABC (UFABC) Universidade Mogi das Cruzes Anhembi Morumbi University Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Sá Alves, Mariana [UNESP] de Sá Rodrigues, Nayara [UNESP] Bandeira, Celso Muller [UNESP] Chagas, José Francisco Sales Pascoal, Maria Beatriz Nogueira Nepomuceno, Gabrielle Luana Jimenez Teodoro da Silva Martinho, Herculano Alves, Mônica Ghislaine Oliveira Mendes, Maria Anita Dias, Meriellen Alves, Levy Anderson César Almeida, Janete Dias [UNESP] |
dc.subject.por.fl_str_mv |
Biomarkers GC-MS Mass spectrometry Metabolites Metabolomics Oral cancer Oral squamous cell carcinoma Saliva |
topic |
Biomarkers GC-MS Mass spectrometry Metabolites Metabolomics Oral cancer Oral squamous cell carcinoma Saliva |
description |
Oral squamous cell carcinoma (OSCC) represents 90% of oral malignant neoplasms. The search for specific biomarkers for OSCC is a very active field of research contributing to establishing early diagnostic methods and unraveling underlying pathogenic mechanisms. In this work we investigated the salivary metabolites and the metabolic pathways of OSCC aiming find possible biomarkers. Salivary metabolites samples from 27 OSCC patients and 41 control individuals were compared through a gas chromatography coupled to a mass spectrometer (GC-MS) technique. Our results allowed identification of pathways of the malate-aspartate shuttle, the beta-alanine metabolism, and the Warburg effect. The possible salivary biomarkers were identified using the area under receiver-operating curve (AUC) criterion. Twenty-four metabolites were identified with AUC > 0.8. Using the threshold of AUC = 0.9 we find malic acid, maltose, protocatechuic acid, lactose, 2-ketoadipic, and catechol metabolites expressed. We notice that this is the first report of salivary metabolome in South American oral cancer patients, to the best of our knowledge. Our findings regarding these metabolic changes are important in discovering salivary biomarkers of OSCC patients. However, additional work needs to be performed considering larger populations to validate our results. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-05-01T09:30:57Z 2022-05-01T09:30:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/metabo11100650 Metabolites, v. 11, n. 10, 2021. 2218-1989 http://hdl.handle.net/11449/233607 10.3390/metabo11100650 2-s2.0-85116077558 |
url |
http://dx.doi.org/10.3390/metabo11100650 http://hdl.handle.net/11449/233607 |
identifier_str_mv |
Metabolites, v. 11, n. 10, 2021. 2218-1989 10.3390/metabo11100650 2-s2.0-85116077558 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Metabolites |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965126727565312 |