Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.gene.2022.147047 http://hdl.handle.net/11449/247964 |
Resumo: | Lung cancer patients with COVID-19 present an increased risk of developing severe disease and, consequently, have poor outcomes. Determining SARS-CoV-2-host interactome in lung cancer cells and tissues, infected or uninfected with SARS-CoV-2, may reveal molecular mechanisms associated with COVID-19 development and severity in lung cancer patients. Here, we integrated transcriptome data of lung tumors from patients with small- or non-small cell lung cancer (SCLC and NSCLC) and normal lung and lung cancer cells infected with SARS-CoV-2. We aimed to characterize molecular mechanisms potentially associated with COVID-19 development and severity in lung cancer patients and to predict the SARS-CoV-2-host cell interactome. We found that the gene expression profiles of lung cell lines infected with SARS-CoV-2 resemble more primary lung tumors than non-malignant lung tissues. In addition, the transcriptomic-based interactome analysis of SCLC and NSCLC revealed increased expression of cancer genes BRCA1 and CENPF, whose proteins are known or predicted to interact with the SARS-CoV-2 spike glycoprotein and helicase, respectively. We also found that TRIB3, a gene coding a putative host-SARS-CoV-2 interacting protein associated with COVID-19 infection, is co-expressed with the up-regulated genes MTHFD2, ADM2, and GPT2 in all tested conditions. Our analysis identified biological processes such as amino acid metabolism and angiogenesis and 22 host mediators of SARS-CoV-2 infection and replication that may contribute to the development and severity of COVID-19 in lung cancers. |
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Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19A549Calu-3Host-virus interactomeNSCLCSARS-CoV-2SCLCLung cancer patients with COVID-19 present an increased risk of developing severe disease and, consequently, have poor outcomes. Determining SARS-CoV-2-host interactome in lung cancer cells and tissues, infected or uninfected with SARS-CoV-2, may reveal molecular mechanisms associated with COVID-19 development and severity in lung cancer patients. Here, we integrated transcriptome data of lung tumors from patients with small- or non-small cell lung cancer (SCLC and NSCLC) and normal lung and lung cancer cells infected with SARS-CoV-2. We aimed to characterize molecular mechanisms potentially associated with COVID-19 development and severity in lung cancer patients and to predict the SARS-CoV-2-host cell interactome. We found that the gene expression profiles of lung cell lines infected with SARS-CoV-2 resemble more primary lung tumors than non-malignant lung tissues. In addition, the transcriptomic-based interactome analysis of SCLC and NSCLC revealed increased expression of cancer genes BRCA1 and CENPF, whose proteins are known or predicted to interact with the SARS-CoV-2 spike glycoprotein and helicase, respectively. We also found that TRIB3, a gene coding a putative host-SARS-CoV-2 interacting protein associated with COVID-19 infection, is co-expressed with the up-regulated genes MTHFD2, ADM2, and GPT2 in all tested conditions. Our analysis identified biological processes such as amino acid metabolism and angiogenesis and 22 host mediators of SARS-CoV-2 infection and replication that may contribute to the development and severity of COVID-19 in lung cancers.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Botucatu, SPDepartment of Genetics Ribeirão Preto Medical School University of São Paulo (USP), SPCenter for Cell-Based Therapy (CEPID/FAPESP) National Institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq) Regional Blood Center of Ribeirão Preto, SPInstitute for Cancer Research (IPEC), PRDepartment of Surgery and Orthopedics Faculty of Medicine São Paulo State University (UNESP), SPDepartment of Immunology Institute of Biomedical Sciences University of São Paulo, SPDepartment of Clinical and Toxicological Analyses School of Pharmaceutical Sciences University of São Paulo (USP), SPNetwork of Immunity in Infection Malignancy and Autoimmunity (NIIMA) Universal Scientific Education and Research Network (USERN), SPDepartment of Pharmacy and Postgraduate Program of Health and Science Federal University of Rio Grande do Norte (UFRN), RNLead ContactDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Botucatu, SPDepartment of Surgery and Orthopedics Faculty of Medicine São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Regional Blood Center of Ribeirão PretoInstitute for Cancer Research (IPEC)Universal Scientific Education and Research Network (USERN)Federal University of Rio Grande do Norte (UFRN)Lead ContactCury, S. S. [UNESP]Oliveira, J. S. [UNESP]Biagi-Júnior, C. A.O.Silva, W. A.Reis, P. P. [UNESP]Cabral-Marques, O.Hasimoto, E. N. [UNESP]Freire, P. P. [UNESP]Carvalho, R. F. [UNESP]2023-07-29T13:30:46Z2023-07-29T13:30:46Z2023-02-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.gene.2022.147047Gene, v. 852.1879-00380378-1119http://hdl.handle.net/11449/24796410.1016/j.gene.2022.1470472-s2.0-85142878063Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneinfo:eu-repo/semantics/openAccess2023-07-29T13:30:46Zoai:repositorio.unesp.br:11449/247964Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:30:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
title |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
spellingShingle |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 Cury, S. S. [UNESP] A549 Calu-3 Host-virus interactome NSCLC SARS-CoV-2 SCLC |
title_short |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
title_full |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
title_fullStr |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
title_full_unstemmed |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
title_sort |
Transcriptional profiles and common genes link lung cancer with the development and severity of COVID-19 |
author |
Cury, S. S. [UNESP] |
author_facet |
Cury, S. S. [UNESP] Oliveira, J. S. [UNESP] Biagi-Júnior, C. A.O. Silva, W. A. Reis, P. P. [UNESP] Cabral-Marques, O. Hasimoto, E. N. [UNESP] Freire, P. P. [UNESP] Carvalho, R. F. [UNESP] |
author_role |
author |
author2 |
Oliveira, J. S. [UNESP] Biagi-Júnior, C. A.O. Silva, W. A. Reis, P. P. [UNESP] Cabral-Marques, O. Hasimoto, E. N. [UNESP] Freire, P. P. [UNESP] Carvalho, R. F. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Regional Blood Center of Ribeirão Preto Institute for Cancer Research (IPEC) Universal Scientific Education and Research Network (USERN) Federal University of Rio Grande do Norte (UFRN) Lead Contact |
dc.contributor.author.fl_str_mv |
Cury, S. S. [UNESP] Oliveira, J. S. [UNESP] Biagi-Júnior, C. A.O. Silva, W. A. Reis, P. P. [UNESP] Cabral-Marques, O. Hasimoto, E. N. [UNESP] Freire, P. P. [UNESP] Carvalho, R. F. [UNESP] |
dc.subject.por.fl_str_mv |
A549 Calu-3 Host-virus interactome NSCLC SARS-CoV-2 SCLC |
topic |
A549 Calu-3 Host-virus interactome NSCLC SARS-CoV-2 SCLC |
description |
Lung cancer patients with COVID-19 present an increased risk of developing severe disease and, consequently, have poor outcomes. Determining SARS-CoV-2-host interactome in lung cancer cells and tissues, infected or uninfected with SARS-CoV-2, may reveal molecular mechanisms associated with COVID-19 development and severity in lung cancer patients. Here, we integrated transcriptome data of lung tumors from patients with small- or non-small cell lung cancer (SCLC and NSCLC) and normal lung and lung cancer cells infected with SARS-CoV-2. We aimed to characterize molecular mechanisms potentially associated with COVID-19 development and severity in lung cancer patients and to predict the SARS-CoV-2-host cell interactome. We found that the gene expression profiles of lung cell lines infected with SARS-CoV-2 resemble more primary lung tumors than non-malignant lung tissues. In addition, the transcriptomic-based interactome analysis of SCLC and NSCLC revealed increased expression of cancer genes BRCA1 and CENPF, whose proteins are known or predicted to interact with the SARS-CoV-2 spike glycoprotein and helicase, respectively. We also found that TRIB3, a gene coding a putative host-SARS-CoV-2 interacting protein associated with COVID-19 infection, is co-expressed with the up-regulated genes MTHFD2, ADM2, and GPT2 in all tested conditions. Our analysis identified biological processes such as amino acid metabolism and angiogenesis and 22 host mediators of SARS-CoV-2 infection and replication that may contribute to the development and severity of COVID-19 in lung cancers. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:30:46Z 2023-07-29T13:30:46Z 2023-02-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.gene.2022.147047 Gene, v. 852. 1879-0038 0378-1119 http://hdl.handle.net/11449/247964 10.1016/j.gene.2022.147047 2-s2.0-85142878063 |
url |
http://dx.doi.org/10.1016/j.gene.2022.147047 http://hdl.handle.net/11449/247964 |
identifier_str_mv |
Gene, v. 852. 1879-0038 0378-1119 10.1016/j.gene.2022.147047 2-s2.0-85142878063 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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