Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1365-3059.2005.01161.x http://hdl.handle.net/11449/5270 |
Resumo: | A new rot caused by a binucleate Rhizoctonia sp. affecting the tuberous root cortex of the domesticated yacon (Smallanthus sonchifolius) has been observed in Brazil. Isolates of a binucleate Rhizoctonia sp. were collected from roots with rot symptoms and characterized by the number of nuclei per cell, hyphal anastomosis, RAPD molecular markers, ITS-5.8S rDNA sequence and pathogenicity tests. All isolates had a mean of 1.9-2.2 nuclei per cell and anastomosed with the binucleate Rhizoctonia sp. AG G-tester strain. RAPD analysis was carried out between 11 isolates recovered from yacon and 11 AG (A, Ba, Bb, Bo, C, D, F, G, O, P, Q) standard testers of binucleate Rhizoctonia sp. Genetic similarities of 94.8-100% were observed among isolates of the binucleate Rhizoctonia sp. from yacon and all isolates were genetically more closely related to the AG G tester than other strains according to UPGMA analysis using RAPD markers. Homologies of complete ITS nucleotide sequences were 100% between binucleate isolates of Rhizoctonia sp. from yacon and the AG G tester. According to pathogenicity tests, the isolates caused typical rot symptoms of yacon tubers 90 days after inoculation. |
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spelling |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazilanastomosis groupingITSRAPDrDNAA new rot caused by a binucleate Rhizoctonia sp. affecting the tuberous root cortex of the domesticated yacon (Smallanthus sonchifolius) has been observed in Brazil. Isolates of a binucleate Rhizoctonia sp. were collected from roots with rot symptoms and characterized by the number of nuclei per cell, hyphal anastomosis, RAPD molecular markers, ITS-5.8S rDNA sequence and pathogenicity tests. All isolates had a mean of 1.9-2.2 nuclei per cell and anastomosed with the binucleate Rhizoctonia sp. AG G-tester strain. RAPD analysis was carried out between 11 isolates recovered from yacon and 11 AG (A, Ba, Bb, Bo, C, D, F, G, O, P, Q) standard testers of binucleate Rhizoctonia sp. Genetic similarities of 94.8-100% were observed among isolates of the binucleate Rhizoctonia sp. from yacon and all isolates were genetically more closely related to the AG G tester than other strains according to UPGMA analysis using RAPD markers. Homologies of complete ITS nucleotide sequences were 100% between binucleate isolates of Rhizoctonia sp. from yacon and the AG G tester. According to pathogenicity tests, the isolates caused typical rot symptoms of yacon tubers 90 days after inoculation.Cent Bur Schimmelcultures, NL-3584 CT Utrecht, NetherlandsMAPA, DFA GO, Lab Apoio Vegetal, BR-74003010 Goiania, Go, BrazilUniv Estadual Paulista Julio Mesquita Filho, Fac Ciências Agron, Dept Prod Vegetal Defesa Fitossanit, BR-18603970 Botucatu, SP, BrazilUniv Estadual Paulista Julio Mesquita Filho, Fac Ciências Agron, Dept Prod Vegetal Defesa Fitossanit, BR-18603970 Botucatu, SP, BrazilBlackwell PublishingCent Bur SchimmelculturesMAPAUniversidade Estadual Paulista (Unesp)Fenille, R. C.Ciampi, M. B.Souza, N. L.Nakatani, A. K.Kuramae, E. E.2014-05-20T13:19:44Z2014-05-20T13:19:44Z2005-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article325-330application/pdfhttp://dx.doi.org/10.1111/j.1365-3059.2005.01161.xPlant Pathology. Oxford: Blackwell Publishing, v. 54, n. 3, p. 325-330, 2005.0032-0862http://hdl.handle.net/11449/527010.1111/j.1365-3059.2005.01161.xWOS:000229368600007WOS000229368600007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Pathology2.3031,063info:eu-repo/semantics/openAccess2024-04-30T15:56:03Zoai:repositorio.unesp.br:11449/5270Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T15:56:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
title |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
spellingShingle |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil Fenille, R. C. anastomosis grouping ITS RAPD rDNA |
title_short |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
title_full |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
title_fullStr |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
title_full_unstemmed |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
title_sort |
Binucleate Rhizoctonia sp AG G causing root rot in yacon (Smallanthus sonchifolius) in Brazil |
author |
Fenille, R. C. |
author_facet |
Fenille, R. C. Ciampi, M. B. Souza, N. L. Nakatani, A. K. Kuramae, E. E. |
author_role |
author |
author2 |
Ciampi, M. B. Souza, N. L. Nakatani, A. K. Kuramae, E. E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Cent Bur Schimmelcultures MAPA Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Fenille, R. C. Ciampi, M. B. Souza, N. L. Nakatani, A. K. Kuramae, E. E. |
dc.subject.por.fl_str_mv |
anastomosis grouping ITS RAPD rDNA |
topic |
anastomosis grouping ITS RAPD rDNA |
description |
A new rot caused by a binucleate Rhizoctonia sp. affecting the tuberous root cortex of the domesticated yacon (Smallanthus sonchifolius) has been observed in Brazil. Isolates of a binucleate Rhizoctonia sp. were collected from roots with rot symptoms and characterized by the number of nuclei per cell, hyphal anastomosis, RAPD molecular markers, ITS-5.8S rDNA sequence and pathogenicity tests. All isolates had a mean of 1.9-2.2 nuclei per cell and anastomosed with the binucleate Rhizoctonia sp. AG G-tester strain. RAPD analysis was carried out between 11 isolates recovered from yacon and 11 AG (A, Ba, Bb, Bo, C, D, F, G, O, P, Q) standard testers of binucleate Rhizoctonia sp. Genetic similarities of 94.8-100% were observed among isolates of the binucleate Rhizoctonia sp. from yacon and all isolates were genetically more closely related to the AG G tester than other strains according to UPGMA analysis using RAPD markers. Homologies of complete ITS nucleotide sequences were 100% between binucleate isolates of Rhizoctonia sp. from yacon and the AG G tester. According to pathogenicity tests, the isolates caused typical rot symptoms of yacon tubers 90 days after inoculation. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06-01 2014-05-20T13:19:44Z 2014-05-20T13:19:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1365-3059.2005.01161.x Plant Pathology. Oxford: Blackwell Publishing, v. 54, n. 3, p. 325-330, 2005. 0032-0862 http://hdl.handle.net/11449/5270 10.1111/j.1365-3059.2005.01161.x WOS:000229368600007 WOS000229368600007.pdf |
url |
http://dx.doi.org/10.1111/j.1365-3059.2005.01161.x http://hdl.handle.net/11449/5270 |
identifier_str_mv |
Plant Pathology. Oxford: Blackwell Publishing, v. 54, n. 3, p. 325-330, 2005. 0032-0862 10.1111/j.1365-3059.2005.01161.x WOS:000229368600007 WOS000229368600007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Pathology 2.303 1,063 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
325-330 application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964810035593216 |