Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00436-019-06362-2 http://hdl.handle.net/11449/187745 |
Resumo: | The Diplostomidae include a large group of flatworms with complex life cycles and are frequently found parasitizing the eyes and central nervous system of freshwater fishes. The morphological identification of the metacercariae at species level is not always possible. Thus, molecular tools have become essential to assist in the parasite species determination. This study was aimed at describing two diplostomid metacercariae found in freshwater fish in São Paulo, Brazil, based on morphological characters and in the genetic characterization of COI sequences. Our results showed that the two recognized taxa (Tylodelphys sp. and Diplostomidae gen. sp.) appear to be different from the species already described in South America. Tylodelphys sp. differs morphologically from Tylodelphys xenopi, T. mashonense, T. jenynsiae, and T. scheuringi. The metacercariae of T. clavata and T. conifera are smaller than Tylodelphys sp., while T. podicipina is larger than the metacercariae described here. The phylogenetic analysis of COI sequences yielded Tylodelphys sp. as the sister species of Tylodelphys sp. 4, a species reported from the brain of the eleotrid Gobiomorus maculatus in Oaxaca, Mexico. The metacercariae identified as Diplostomidae gen. sp. are morphologically different from the known diplostomid metacercariae and did not match with other diplostomid sequences available. Diplostomidae gen. sp. is recovered as the sister species of Diplostomum ardeae. Although the morphological evidence and the COI sequences differentiate the metacercariae found, the absence of adult specimens of both species precludes the specific designation. This is one of the first papers that use an integrative taxonomy approach to describe the species diversity of diplostomid trematodes in Brazil. |
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Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, BrazilBayesian analysisCytochrome c oxidaseFish parasitesFlatwormsMorphological analysisTrematodaThe Diplostomidae include a large group of flatworms with complex life cycles and are frequently found parasitizing the eyes and central nervous system of freshwater fishes. The morphological identification of the metacercariae at species level is not always possible. Thus, molecular tools have become essential to assist in the parasite species determination. This study was aimed at describing two diplostomid metacercariae found in freshwater fish in São Paulo, Brazil, based on morphological characters and in the genetic characterization of COI sequences. Our results showed that the two recognized taxa (Tylodelphys sp. and Diplostomidae gen. sp.) appear to be different from the species already described in South America. Tylodelphys sp. differs morphologically from Tylodelphys xenopi, T. mashonense, T. jenynsiae, and T. scheuringi. The metacercariae of T. clavata and T. conifera are smaller than Tylodelphys sp., while T. podicipina is larger than the metacercariae described here. The phylogenetic analysis of COI sequences yielded Tylodelphys sp. as the sister species of Tylodelphys sp. 4, a species reported from the brain of the eleotrid Gobiomorus maculatus in Oaxaca, Mexico. The metacercariae identified as Diplostomidae gen. sp. are morphologically different from the known diplostomid metacercariae and did not match with other diplostomid sequences available. Diplostomidae gen. sp. is recovered as the sister species of Diplostomum ardeae. Although the morphological evidence and the COI sequences differentiate the metacercariae found, the absence of adult specimens of both species precludes the specific designation. This is one of the first papers that use an integrative taxonomy approach to describe the species diversity of diplostomid trematodes in Brazil.Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP), Rua Prof. Dr. Antônio Celso Wagner Zanin, Distrito de Rubião JúniorPró-reitoria de Pesquisa e Pós-graduação Laboratório de Ictioparasitologia Rua Irmã Arminda Universidade do Sagrado Coração (USC), 10-50, Jardim BrasilDepartamento de Ciências Biológicas Universidade Federal de São Paulo (UNIFESP)Instituto de Biologia Universidad Nacional Autónoma de México (UNAM), Av. Universidad 3000, Cd. Universitaria, CoyoacánPrograma de Pós-Graduação em Análise de Sistemas Ambientais Centro Universitário CESMACInstituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP), Rua Prof. Dr. Antônio Celso Wagner Zanin, Distrito de Rubião JúniorUniversidade Estadual Paulista (Unesp)Universidade do Sagrado Coração (USC)Universidade Federal de São Paulo (UNIFESP)Universidad Nacional Autónoma de México (UNAM)Centro Universitário CESMACPelegrini, Larissa Sbeghen [UNESP]Gião, ThayanaVieira, Diego Henrique Mirandola Dias [UNESP]Müller, Maria Isabel [UNESP]da Silva, Reinaldo José [UNESP]de León, Gerardo Pérez-Poncede Azevedo, Rodney KozlowiskiAbdallah, Vanessa Doro2019-10-06T15:45:53Z2019-10-06T15:45:53Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2169-2182http://dx.doi.org/10.1007/s00436-019-06362-2Parasitology Research, v. 118, n. 7, p. 2169-2182, 2019.1432-19550932-0113http://hdl.handle.net/11449/18774510.1007/s00436-019-06362-22-s2.0-85067273702Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2021-10-22T21:16:13Zoai:repositorio.unesp.br:11449/187745Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:16:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
title |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
spellingShingle |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil Pelegrini, Larissa Sbeghen [UNESP] Bayesian analysis Cytochrome c oxidase Fish parasites Flatworms Morphological analysis Trematoda |
title_short |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
title_full |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
title_fullStr |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
title_full_unstemmed |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
title_sort |
Molecular and morphological characterization of the metacercariae of two species of diplostomid trematodes (Platyhelminthes, Digenea) in freshwater fishes of the Batalha River, Brazil |
author |
Pelegrini, Larissa Sbeghen [UNESP] |
author_facet |
Pelegrini, Larissa Sbeghen [UNESP] Gião, Thayana Vieira, Diego Henrique Mirandola Dias [UNESP] Müller, Maria Isabel [UNESP] da Silva, Reinaldo José [UNESP] de León, Gerardo Pérez-Ponce de Azevedo, Rodney Kozlowiski Abdallah, Vanessa Doro |
author_role |
author |
author2 |
Gião, Thayana Vieira, Diego Henrique Mirandola Dias [UNESP] Müller, Maria Isabel [UNESP] da Silva, Reinaldo José [UNESP] de León, Gerardo Pérez-Ponce de Azevedo, Rodney Kozlowiski Abdallah, Vanessa Doro |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade do Sagrado Coração (USC) Universidade Federal de São Paulo (UNIFESP) Universidad Nacional Autónoma de México (UNAM) Centro Universitário CESMAC |
dc.contributor.author.fl_str_mv |
Pelegrini, Larissa Sbeghen [UNESP] Gião, Thayana Vieira, Diego Henrique Mirandola Dias [UNESP] Müller, Maria Isabel [UNESP] da Silva, Reinaldo José [UNESP] de León, Gerardo Pérez-Ponce de Azevedo, Rodney Kozlowiski Abdallah, Vanessa Doro |
dc.subject.por.fl_str_mv |
Bayesian analysis Cytochrome c oxidase Fish parasites Flatworms Morphological analysis Trematoda |
topic |
Bayesian analysis Cytochrome c oxidase Fish parasites Flatworms Morphological analysis Trematoda |
description |
The Diplostomidae include a large group of flatworms with complex life cycles and are frequently found parasitizing the eyes and central nervous system of freshwater fishes. The morphological identification of the metacercariae at species level is not always possible. Thus, molecular tools have become essential to assist in the parasite species determination. This study was aimed at describing two diplostomid metacercariae found in freshwater fish in São Paulo, Brazil, based on morphological characters and in the genetic characterization of COI sequences. Our results showed that the two recognized taxa (Tylodelphys sp. and Diplostomidae gen. sp.) appear to be different from the species already described in South America. Tylodelphys sp. differs morphologically from Tylodelphys xenopi, T. mashonense, T. jenynsiae, and T. scheuringi. The metacercariae of T. clavata and T. conifera are smaller than Tylodelphys sp., while T. podicipina is larger than the metacercariae described here. The phylogenetic analysis of COI sequences yielded Tylodelphys sp. as the sister species of Tylodelphys sp. 4, a species reported from the brain of the eleotrid Gobiomorus maculatus in Oaxaca, Mexico. The metacercariae identified as Diplostomidae gen. sp. are morphologically different from the known diplostomid metacercariae and did not match with other diplostomid sequences available. Diplostomidae gen. sp. is recovered as the sister species of Diplostomum ardeae. Although the morphological evidence and the COI sequences differentiate the metacercariae found, the absence of adult specimens of both species precludes the specific designation. This is one of the first papers that use an integrative taxonomy approach to describe the species diversity of diplostomid trematodes in Brazil. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:45:53Z 2019-10-06T15:45:53Z 2019-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00436-019-06362-2 Parasitology Research, v. 118, n. 7, p. 2169-2182, 2019. 1432-1955 0932-0113 http://hdl.handle.net/11449/187745 10.1007/s00436-019-06362-2 2-s2.0-85067273702 |
url |
http://dx.doi.org/10.1007/s00436-019-06362-2 http://hdl.handle.net/11449/187745 |
identifier_str_mv |
Parasitology Research, v. 118, n. 7, p. 2169-2182, 2019. 1432-1955 0932-0113 10.1007/s00436-019-06362-2 2-s2.0-85067273702 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2169-2182 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789392022011904 |