Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules21010037 http://hdl.handle.net/11449/178447 |
Resumo: | Decapeptide KSL-W shows antibacterial activities and can be used in the oral cavity, however, it is easily degraded in aqueous solution and eliminated. Therefore, we aimed to develop liquid crystalline systems (F1 and F2) for KSL-W buccal administration to treat multispecies oral biofilms. The systems were prepared with oleic acid, polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20), and a 1% poloxamer 407 dispersion as the oil phase (OP), surfactant (S), and aqueous phase (AP), respectively. We characterized them using polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, and in vitro bioadhesion, and performed in vitro biological analysis. PLM showed isotropy (F1) or anisotropy with lamellar mesophases (F2), confirmed by peak ratio quantification using SAXS. Rheological tests demonstrated that F1 exhibited Newtonian behavior but not F2, which showed a structured AP concentration-dependent system. Bioadhesion studies revealed an AP concentration-dependent increase in the system's bioadhesiveness (F2 = 15.50 ±1.00 mN s) to bovine teeth blocks. Antimicrobial testing revealed 100% inhibition of multispecies oral biofilm growth after KSL-W administration, which was incorporated in the F2 aqueous phase at a concentration of 1 mg/mL. Our results suggest that this system could serve as a potential vehicle for buccal administration of antibiofilm peptides. |
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Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilmAntimicrobial peptideBiofilmKSL-WLiquid crystalline systemsOral cavityDecapeptide KSL-W shows antibacterial activities and can be used in the oral cavity, however, it is easily degraded in aqueous solution and eliminated. Therefore, we aimed to develop liquid crystalline systems (F1 and F2) for KSL-W buccal administration to treat multispecies oral biofilms. The systems were prepared with oleic acid, polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20), and a 1% poloxamer 407 dispersion as the oil phase (OP), surfactant (S), and aqueous phase (AP), respectively. We characterized them using polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, and in vitro bioadhesion, and performed in vitro biological analysis. PLM showed isotropy (F1) or anisotropy with lamellar mesophases (F2), confirmed by peak ratio quantification using SAXS. Rheological tests demonstrated that F1 exhibited Newtonian behavior but not F2, which showed a structured AP concentration-dependent system. Bioadhesion studies revealed an AP concentration-dependent increase in the system's bioadhesiveness (F2 = 15.50 ±1.00 mN s) to bovine teeth blocks. Antimicrobial testing revealed 100% inhibition of multispecies oral biofilm growth after KSL-W administration, which was incorporated in the F2 aqueous phase at a concentration of 1 mg/mL. Our results suggest that this system could serve as a potential vehicle for buccal administration of antibiofilm peptides.School of Pharmaceutical Sciences Sao Paulo State University UNESP, Rodovia Araraquara-Jaú Km 01Chemistry Institute UNESP Sao Paulo State UniversitySchool of Pharmaceutical Sciences Sao Paulo State University UNESP, Rodovia Araraquara-Jaú Km 01Chemistry Institute UNESP Sao Paulo State UniversityUniversidade Estadual Paulista (Unesp)Bernegossi, Jéssica [UNESP]Calixto, Giovana Maria Fioramonti [UNESP]Da Silva Sanches, Paulo Ricardo [UNESP]Fontana, Carla Raquel [UNESP]Cilli, Eduardo Maffud [UNESP]Garrido, Saulo Santesso [UNESP]Chorilli, Marlus [UNESP]2018-12-11T17:30:23Z2018-12-11T17:30:23Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/molecules21010037Molecules, v. 21, n. 1, 2016.1420-3049http://hdl.handle.net/11449/17844710.3390/molecules210100372-s2.0-850007833462-s2.0-85000783346.pdf1427125996716282Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules0,855info:eu-repo/semantics/openAccess2023-11-26T06:13:17Zoai:repositorio.unesp.br:11449/178447Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-26T06:13:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
title |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
spellingShingle |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm Bernegossi, Jéssica [UNESP] Antimicrobial peptide Biofilm KSL-W Liquid crystalline systems Oral cavity |
title_short |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
title_full |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
title_fullStr |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
title_full_unstemmed |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
title_sort |
Peptide KSL-W-loaded mucoadhesive liquid crystalline vehicle as an alternative treatment for multispecies oral biofilm |
author |
Bernegossi, Jéssica [UNESP] |
author_facet |
Bernegossi, Jéssica [UNESP] Calixto, Giovana Maria Fioramonti [UNESP] Da Silva Sanches, Paulo Ricardo [UNESP] Fontana, Carla Raquel [UNESP] Cilli, Eduardo Maffud [UNESP] Garrido, Saulo Santesso [UNESP] Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Calixto, Giovana Maria Fioramonti [UNESP] Da Silva Sanches, Paulo Ricardo [UNESP] Fontana, Carla Raquel [UNESP] Cilli, Eduardo Maffud [UNESP] Garrido, Saulo Santesso [UNESP] Chorilli, Marlus [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bernegossi, Jéssica [UNESP] Calixto, Giovana Maria Fioramonti [UNESP] Da Silva Sanches, Paulo Ricardo [UNESP] Fontana, Carla Raquel [UNESP] Cilli, Eduardo Maffud [UNESP] Garrido, Saulo Santesso [UNESP] Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial peptide Biofilm KSL-W Liquid crystalline systems Oral cavity |
topic |
Antimicrobial peptide Biofilm KSL-W Liquid crystalline systems Oral cavity |
description |
Decapeptide KSL-W shows antibacterial activities and can be used in the oral cavity, however, it is easily degraded in aqueous solution and eliminated. Therefore, we aimed to develop liquid crystalline systems (F1 and F2) for KSL-W buccal administration to treat multispecies oral biofilms. The systems were prepared with oleic acid, polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20), and a 1% poloxamer 407 dispersion as the oil phase (OP), surfactant (S), and aqueous phase (AP), respectively. We characterized them using polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, and in vitro bioadhesion, and performed in vitro biological analysis. PLM showed isotropy (F1) or anisotropy with lamellar mesophases (F2), confirmed by peak ratio quantification using SAXS. Rheological tests demonstrated that F1 exhibited Newtonian behavior but not F2, which showed a structured AP concentration-dependent system. Bioadhesion studies revealed an AP concentration-dependent increase in the system's bioadhesiveness (F2 = 15.50 ±1.00 mN s) to bovine teeth blocks. Antimicrobial testing revealed 100% inhibition of multispecies oral biofilm growth after KSL-W administration, which was incorporated in the F2 aqueous phase at a concentration of 1 mg/mL. Our results suggest that this system could serve as a potential vehicle for buccal administration of antibiofilm peptides. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:30:23Z 2018-12-11T17:30:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules21010037 Molecules, v. 21, n. 1, 2016. 1420-3049 http://hdl.handle.net/11449/178447 10.3390/molecules21010037 2-s2.0-85000783346 2-s2.0-85000783346.pdf 1427125996716282 |
url |
http://dx.doi.org/10.3390/molecules21010037 http://hdl.handle.net/11449/178447 |
identifier_str_mv |
Molecules, v. 21, n. 1, 2016. 1420-3049 10.3390/molecules21010037 2-s2.0-85000783346 2-s2.0-85000783346.pdf 1427125996716282 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965076932788224 |